LOCUS       Exported     3349 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3349
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1387..1434
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3282..3298
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3299..3330
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
                     /label="tonB terminator"
     CDS             29..1315
                     /label="MPN101"
                     /note=""
                     /translation="MRDNSAKGITAGSESQQTTYDPTRTEAALTASTTFALRRYDLAGRALYDLDFSRLNPQTPTRDQTGQITFNPFGGFGLSGAAPQQWNEVKNKVPVEVAQDPSNPYRFAVLLVPRSVVYYEQLQRGLALPNQGSSSGSGQQNTTIGAYGLKVKNAEADTAKSNEKLQGDESKSSNGSSSTSTTTQRGGSSGDTKVKALQVAVKKKSGSQGNSGEQGTEQVELESNDLANAPIKRGEESGQSVQLKAADFGTTPSSSGSGGNSNPGSPTPWRPWLATEQIHKDLPKWSASILILYDAPYARNRTAIDRVDHLDPKVMTANYPPSWRTPKWNHHGLWDWKARDVLLQTTGFFNSRRHPEWFDQGQAVADNTQTGFDTDDTDNKKTRLSKGSWLRQAGPDRPPVWSVLRQHWQPHLVRASAFGVWDLFVLIN"
     primer_bind     complement(1439..1455)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1522..2110)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2301..3161)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3162..3266)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 AAACACGTGG CAAACATTCC GGTCTCAAAT GCGTGACAAT AGCGCCAAAG GTATCACCGC
       61 GGGTTCGGAA TCGCAACAAA CCACGTACGA CCCGACCCGC ACCGAGGCAG CGTTAACGGC
      121 GAGCACGACC TTTGCGTTGC GCCGTTATGA CCTGGCGGGT CGTGCCTTGT ACGACTTGGA
      181 CTTTTCCCGT CTGAATCCGC AAACGCCGAC CCGCGATCAG ACGGGCCAAA TCACCTTCAA
      241 TCCGTTCGGT GGTTTTGGTT TGAGCGGTGC GGCACCGCAA CAATGGAACG AGGTCAAGAA
      301 TAAAGTGCCG GTCGAAGTGG CCCAGGACCC GAGCAACCCG TACCGCTTCG CTGTTCTTTT
      361 GGTGCCGCGT AGCGTTGTGT ACTATGAACA GCTGCAACGT GGCTTGGCTC TGCCGAATCA
      421 AGGTAGCAGC AGCGGTTCTG GTCAACAGAA TACCACCATC GGTGCCTATG GTCTGAAAGT
      481 TAAGAATGCG GAAGCGGATA CGGCGAAAAG CAACGAGAAG CTGCAGGGTG ATGAGTCCAA
      541 AAGCAGCAAT GGCTCCAGCT CCACGAGCAC GACGACCCAG CGTGGCGGCA GCAGCGGTGA
      601 CACGAAAGTG AAGGCCCTTC AGGTTGCGGT CAAGAAAAAG AGCGGTAGCC AAGGCAATAG
      661 CGGCGAACAG GGTACTGAGC AAGTGGAGCT GGAAAGCAAC GACCTGGCGA ATGCGCCGAT
      721 CAAACGTGGT GAGGAAAGCG GCCAAAGCGT TCAACTGAAA GCGGCGGATT TCGGTACTAC
      781 CCCTAGCAGC AGCGGTAGCG GTGGTAATTC CAACCCGGGT TCACCAACCC CGTGGCGCCC
      841 GTGGCTGGCT ACCGAGCAGA TCCACAAAGA TCTGCCGAAA TGGTCTGCAT CGATCTTGAT
      901 TCTGTACGAC GCGCCATATG CCCGCAACCG CACGGCGATT GATCGCGTGG ACCACCTGGA
      961 CCCGAAAGTC ATGACCGCCA ACTACCCGCC GAGCTGGCGC ACCCCGAAAT GGAACCACCA
     1021 TGGTCTGTGG GATTGGAAAG CGCGCGACGT TCTGCTGCAG ACGACGGGCT TCTTTAACAG
     1081 CCGTCGTCAC CCAGAATGGT TTGACCAGGG TCAGGCAGTG GCCGACAATA CGCAGACCGG
     1141 CTTCGATACG GATGATACCG ATAATAAAAA GACGCGTTTG AGCAAGGGTA GCTGGCTGCG
     1201 TCAGGCCGGC CCGGACCGTC CGCCGGTTTG GTCTGTTCTG CGCCAACACT GGCAACCGCA
     1261 CCTGGTTCGT GCGAGCGCCT TCGGTGTGTG GGACTTGTTC GTTCTGATTA ACTGAAGAGC
     1321 TTAGAGACCC GAGAGTGAGC ATCATCGTTC GCTGAGGTGT CAATCGTCGG AGCCGCTGAG
     1381 CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCATGGT
     1441 CATAGCTGTT TCCTGAGAGC TTGGCAGGTG ATGACACACA TTAACAAATT TCGTGAGGAG
     1501 TCTCCAGAAG AATGCCATTA ATTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA
     1561 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC
     1621 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC
     1681 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG
     1741 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA
     1801 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC
     1861 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC
     1921 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG
     1981 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA
     2041 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA
     2101 AGGATCTCAA GAAGGCCTAC TATTAGCAAC AACGATCCTT TGATCTTTTC TACGGGGTCT
     2161 GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG
     2221 ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT
     2281 GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC
     2341 TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG
     2401 GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGAACCACG CTCACCGGCT
     2461 CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA
     2521 ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG
     2581 CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG
     2641 TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC
     2701 CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG
     2761 TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG
     2821 CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG
     2881 TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT
     2941 AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG
     3001 ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA
     3061 GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA
     3121 AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT
     3181 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG
     3241 AAAAATAAAC AAATAGGGGT TCCGCGCACC TGCACCAGTC AGTAAAACGA CGGCCAGTAG
     3301 TCAAAAGCCT CCGACCGGAG GCTTTTGACT TGGTCAGGTG GAGTGGGAG
//