LOCUS       Exported     3088 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3088
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1126..1173
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3021..3037
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3038..3069
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..1054
                     /translation="MIDQSKLIERWKTTFETAQNPTELLAFKNSFRNADLKPLLSQIKETTDIETKRHLGQLYKQLESTLQTLHDTQLQVFTQAQSSSVLTHGDVMLLATSFAPGSSNIIYQVIDELVNYFKKFLFTVNYDSELTTIADCFDLLNIPKDHPSRNLTDTFYLDKNRLLRTHCTAATLRAVKETKKSNNPDIRIASFGAVFRKDDDDATHSHQFNQLDFMWIKKDFSLTNLKWFMQNMINHIFGENTSARFRLSHFPFTEPSFEIDIRCWLCQNGCGVCKKTRWIEVLGAGILHPQVMANMGFSDTDNIRGIAAGIGIERLVMLKHGISDIRDLYDNNFKFLAQFTD"
                     /label="pheS"
                     /note=""
     primer_bind     complement(1178..1194)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1261..1849)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2040..2900)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(2901..3005)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 AAGAATTACT GACCCCTCGG GGTCTCAAAT GATCGACCAA TCAAAACTGA TTGAACGTTG
       61 GAAAACCACG TTCGAAACCG CGCAGAACCC GACGGAACTG TTAGCATTCA AGAACTCGTT
      121 TCGTAATGCC GACCTGAAAC CGCTCCTGAG CCAGATTAAA GAAACCACCG ACATCGAAAC
      181 CAAGCGTCAC CTGGGCCAGC TGTACAAGCA ACTGGAGTCT ACCCTGCAGA CCCTGCACGA
      241 CACCCAGCTT CAGGTGTTCA CTCAGGCGCA ATCTTCTTCC GTGCTGACCC ACGGTGACGT
      301 GATGCTGCTG GCTACCAGCT TCGCGCCGGG CAGCTCTAAT ATTATTTATC AGGTGATTGA
      361 CGAATTGGTC AACTACTTCA AAAAGTTTTT GTTTACGGTT AACTACGATT CCGAACTGAC
      421 CACGATTGCG GATTGCTTCG ATCTGCTGAA TATCCCGAAA GATCACCCGT CCCGTAACCT
      481 GACCGATACC TTTTACCTGG ATAAAAACCG CCTGCTGCGT ACTCACTGTA CTGCGGCAAC
      541 GCTGCGCGCG GTGAAAGAAA CGAAGAAAAG CAACAATCCG GACATTAGAA TTGCATCATT
      601 CGGCGCGGTG TTCCGCAAGG ATGATGACGA TGCTACCCAC TCCCACCAAT TCAATCAGCT
      661 GGACTTTATG TGGATTAAAA AAGATTTTAG CTTAACGAAT CTTAAATGGT TTATGCAAAA
      721 TATGATCAAC CACATTTTCG GCGAGAATAC CAGCGCCCGT TTTCGTCTGA GCCATTTCCC
      781 GTTTACCGAA CCGAGCTTCG AGATCGACAT CCGTTGCTGG CTGTGCCAGA ACGGTTGCGG
      841 TGTTTGTAAG AAAACCCGTT GGATTGAGGT GTTGGGCGCG GGTATTCTGC ACCCACAGGT
      901 TATGGCAAAT ATGGGCTTTA GCGACACGGA CAATATCCGC GGTATCGCGG CTGGCATTGG
      961 CATTGAACGT CTGGTGATGC TGAAACATGG CATCAGCGAC ATTCGTGATT TATATGATAA
     1021 TAACTTCAAG TTCCTGGCGC AGTTTACTGA TTGAAGAGCT TAGAGACCCT GTATCATGGT
     1081 TCCCTCGGCG CTGAGGTGTC AATCGTCGGA GCCGCTGAGC AATAACTAGC ATAACCCCTT
     1141 GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCATGGTC ATAGCTGTTT CCTGAGAGCT
     1201 TGGCAGGTGA TGACACACAT TAACAAATTT CGTGAGGAGT CTCCAGAAGA ATGCCATTAA
     1261 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG
     1321 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG
     1381 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG
     1441 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC
     1501 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA
     1561 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG
     1621 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC
     1681 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC
     1741 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG
     1801 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGGCCTACT
     1861 ATTAGCAACA ACGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC
     1921 TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA
     1981 AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT
     2041 TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA
     2101 GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC
     2161 AGTGCTGCAA TGATACCGCG AGAACCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC
     2221 CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG
     2281 TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC
     2341 GTTGTTGCCA TTGCTACAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC
     2401 AGCTCCGGTT CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG
     2461 GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC
     2521 ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT
     2581 GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC
     2641 TCTTGCCCGG CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC
     2701 ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC
     2761 AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC
     2821 GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA
     2881 CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT
     2941 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT
     3001 CCGCGCACCT GCACCAGTCA GTAAAACGAC GGCCAGTAGT CAAAAGCCTC CGACCGGAGG
     3061 CTTTTGACTT GGTCAGGTGG AGTGGGAG
//