LOCUS Exported 4219 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..4219 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 2257..2304 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 4152..4168 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 4169..4200 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 29..2185 /label="MPN110" /note="" /translation="MKIRISLNILAKFLDSFAHLLFLKNKEIYTPRKEQAACVEVLERYFQANPENGRFLMNCKMRFGKCFTLYSYAQKNNINKILILTFVPAVEESLKDDLNHIEKNYKFYTDDDLQKSNFDLKNQNEPYVVFLSLQNVLGKQRIDGAKTDFDKERISKLQEIDFDLIVFDEYHYGANKKRTQIKVEKVNKKIDNPEQQDNQDDAEEELASTFKLKDIKSKFQFSYKQLVCLSGTPFSSLRNNEFSSKDQVFTYSYFDEQKAKSAENHPLKLGQYGIFPEMNIYCFELAEIFTAQEQEIFITPGKGKNKLPEISFRKLFQTENVSKESSKPVYRFVNENLVEKLIDSLIDKRKGFSHTPLSWENIDKHKHSLLILPTRVACFALANLLKNHWYFENNDFQIINMSESQFGNGKKALIELNKHLDEAKKTNKNTLTITVAKLTIGITVKEWSTVFFLKDLKGAESYFQTIFRIQTPYIKNCKNLKEICYVYDFNMYRCLEVTNEYSKQTQTDPKFSASWFQNLDKFLPIYLVRGDEIQKTDPEILQKYEYFIMDKRAFSTRWMDESNIIDIDVLCNVGQDEDAQKILKKILAHKKFKSSKKKREFEDVHLEKSPKSEAFSEGVRSGKDYAAEQGNVLEELENFWNFNQALEQKAKAEFQQKNFDDNEWNNYKKGFNFGVNKHFEDKVSIKKIVQNKIKDFKKRKGRTTSTFKKWKWLYFWWE" primer_bind complement(2309..2325) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(2392..2980) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(3171..4031) /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" /label="bla" promoter complement(4032..4136) /label="bla promoter" /note="ampicillin resistance gene promoter" ORIGIN 1 AAACACGTGG CAAACATTCC GGTCTCAAAT GAAGATCCGC ATCAGCTTGA ATATCCTGGC 61 AAAATTCCTT GATAGCTTTG CCCATCTGCT GTTTCTGAAG AATAAGGAGA TTTACACCCC 121 GCGTAAGGAA CAGGCGGCTT GTGTGGAAGT TCTGGAGCGC TACTTTCAAG CGAACCCGGA 181 GAACGGCCGT TTCCTGATGA ATTGTAAAAT GCGTTTCGGC AAGTGCTTCA CTCTGTATAG 241 CTACGCCCAA AAGAATAATA TCAACAAAAT TCTGATTTTG ACGTTTGTTC CGGCAGTTGA 301 AGAGTCTCTG AAAGACGACT TGAATCACAT CGAGAAAAAT TACAAATTTT ACACCGACGA 361 CGATCTGCAA AAAAGCAATT TCGACTTGAA GAACCAGAAT GAACCGTACG TTGTCTTTCT 421 CAGCCTGCAA AACGTGCTGG GCAAGCAACG CATTGATGGT GCGAAAACCG ACTTTGATAA 481 GGAACGCATC AGCAAGCTGC AAGAAATCGA CTTTGACCTG ATTGTCTTTG ACGAATACCA 541 CTACGGCGCG AATAAGAAAC GCACCCAGAT TAAAGTCGAA AAAGTCAATA AGAAAATTGA 601 TAATCCGGAG CAACAGGATA ACCAGGACGA CGCAGAGGAG GAATTGGCCA GCACGTTCAA 661 GCTGAAGGAC ATTAAGAGCA AATTCCAATT CAGCTATAAG CAGCTGGTGT GTCTGAGCGG 721 TACGCCGTTT AGCAGCCTGA GAAATAACGA ATTCAGCTCG AAAGACCAAG TGTTTACCTA 781 CAGCTACTTT GACGAACAGA AAGCCAAGAG CGCCGAGAAC CACCCACTGA AACTCGGTCA 841 ATATGGCATC TTCCCGGAGA TGAACATTTA CTGCTTCGAA CTGGCGGAAA TTTTCACGGC 901 GCAAGAACAA GAAATCTTCA TCACCCCGGG TAAAGGCAAG AATAAGCTGC CGGAAATCAG 961 CTTTCGTAAG CTGTTTCAGA CCGAGAACGT TAGCAAGGAG TCAAGCAAGC CGGTGTACCG 1021 TTTCGTGAAC GAAAATCTGG TTGAGAAGCT CATTGACTCC CTGATTGATA AGCGCAAAGG 1081 CTTTAGCCAC ACCCCGCTGA GCTGGGAAAA TATTGACAAG CACAAACACA GCCTCCTGAT 1141 TTTGCCAACC CGCGTTGCAT GCTTTGCTCT GGCAAATCTG TTGAAAAATC ACTGGTACTT 1201 CGAGAACAAC GATTTCCAAA TTATCAACAT GAGCGAGAGC CAGTTCGGTA ATGGCAAGAA 1261 GGCGTTGATC GAGTTGAATA AACATCTGGA TGAAGCGAAG AAAACCAATA AAAACACTCT 1321 GACGATCACC GTGGCGAAAC TTACCATTGG CATTACCGTT AAAGAATGGA GCACGGTGTT 1381 CTTCCTGAAG GATCTGAAAG GTGCTGAATC GTATTTCCAG ACGATTTTTC GTATCCAAAC 1441 CCCATATATT AAAAACTGCA AGAATCTGAA AGAAATTTGC TACGTGTATG ACTTTAATAT 1501 GTATCGTTGC CTGGAGGTCA CCAACGAGTA CTCTAAGCAA ACGCAGACGG ATCCGAAATT 1561 CTCTGCGAGC TGGTTCCAAA ATCTGGACAA GTTTCTGCCT ATCTACCTGG TTCGTGGTGA 1621 TGAGATCCAA AAGACCGATC CAGAAATTTT GCAAAAGTAC GAATATTTCA TTATGGACAA 1681 GCGTGCATTT AGCACCCGTT GGATGGATGA GTCCAACATT ATCGATATTG ACGTCCTGTG 1741 CAATGTGGGT CAGGACGAGG ACGCGCAAAA GATTTTGAAG AAAATTTTGG CCCATAAGAA 1801 GTTTAAGAGC AGCAAAAAGA AACGTGAATT CGAAGATGTT CATCTGGAAA AGAGCCCGAA 1861 AAGCGAGGCA TTTAGCGAAG GTGTCCGCTC TGGTAAGGAC TATGCAGCCG AGCAGGGTAA 1921 CGTTCTGGAA GAACTGGAAA ATTTTTGGAA CTTTAATCAG GCGCTGGAAC AGAAGGCAAA 1981 AGCCGAATTC CAGCAGAAAA ACTTCGATGA CAACGAGTGG AACAATTATA AGAAAGGTTT 2041 CAATTTCGGT GTCAACAAGC ACTTCGAGGA CAAGGTCAGC ATCAAAAAGA TTGTCCAGAA 2101 TAAAATTAAG GACTTTAAAA AGCGTAAAGG TCGTACCACC AGCACCTTTA AAAAATGGAA 2161 GTGGCTGTAT TTCTGGTGGG AGTGAAGAGC TTAGAGACCG ACCTTGGGAT CGCTTATGTC 2221 GCTGAGGTGT CAATCGTCGG AGCCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT 2281 AAACGGGTCT TGAGGGGTTT TTTGCATGGT CATAGCTGTT TCCTGAGAGC TTGGCAGGTG 2341 ATGACACACA TTAACAAATT TCGTGAGGAG TCTCCAGAAG AATGCCATTA ATTTCCATAG 2401 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 2461 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 2521 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 2581 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 2641 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 2701 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 2761 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 2821 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 2881 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 2941 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGGCCTAC TATTAGCAAC 3001 AACGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA 3061 GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA 3121 TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC 3181 TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA 3241 CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA 3301 ATGATACCGC GAGAACCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC 3361 GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT 3421 TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC 3481 ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT 3541 TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC 3601 TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG 3661 GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT 3721 GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG 3781 GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA 3841 AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG 3901 TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG 3961 TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT 4021 TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 4081 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACC 4141 TGCACCAGTC AGTAAAACGA CGGCCAGTAG TCAAAAGCCT CCGACCGGAG GCTTTTGACT 4201 TGGTCAGGTG GAGTGGGAG //