LOCUS Exported 3823 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..3823 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 1861..1908 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 3756..3772 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 3773..3804 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 29..1789 /label="ugpC" /note="" /translation="MEKIQAEKSQSAIEFKNIVVDFGESIAIDNINLTVKKKELVTLLGPSGCGKTTSLSVIAGLIAPTSGQVLFNGYDVTKKPPQQRKLGLVFQNYALYPHMSVFENIVFPLYSDTSWREAIFEKNTWAQHDINCLILKANGATSEELAELNRLMQQRIDEPKRMAYQINDLMVSVFQKQSELEANLKLIPRKKQFAIISLSKETLSQIRDVETKAKAALETADSAEVEQTIKSELKQKLSEIKANYHDEKANIKAYWWEMLANIKTELKTEKTAIKQTNDYAKLKELKWKIHFEPLNLKKQYRSYFKQLKAKYSLKDGNLTESELSQIEELQKRIVSLKDFINRTAKEVAEKLEITKILHKRPANISGGQQQRVAIARAIVRRPKVLLMDEPLSNLDAKLRVQTRQWIRKFQQDLQITTVFVTHDQEEAMSISDTIVCMSTGKVQQIGSPSELYLKPANEFVATFLGSPEMNIVNATVKAGQLLWNENPLVKTKFDLPDGAIRVGFRYDEVTAPKNDGSPVFSGTLISVENLGKHMVGVVESNGVQLNVRLELSHQFEVGNAVKFTIKPNGLHFFDPQTTQRVEVKHV" primer_bind complement(1913..1929) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" rep_origin complement(1996..2584) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(2775..3635) /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" promoter complement(3636..3740) /label="bla promoter" /note="ampicillin resistance gene promoter" ORIGIN 1 AAGAATTACT GACCCCTCGG GGTCTCAAAT GGAGAAGATC CAAGCAGAAA AAAGCCAATC 61 TGCTATCGAG TTCAAAAACA TTGTGGTCGA TTTCGGCGAG TCGATCGCGA TTGATAACAT 121 CAATTTAACT GTGAAGAAAA AAGAGTTAGT CACCCTGCTG GGTCCGAGCG GTTGCGGTAA 181 AACGACCAGC CTGAGCGTGA TCGCGGGTCT GATTGCGCCA ACGAGCGGTC AGGTCCTCTT 241 TAATGGTTAC GATGTAACGA AGAAACCGCC GCAACAGAGA AAGCTGGGTC TGGTTTTCCA 301 AAACTACGCG CTGTACCCGC ACATGAGCGT TTTTGAGAAC ATCGTGTTCC CGTTGTACAG 361 CGATACCTCT TGGCGTGAAG CAATTTTTGA GAAAAATACC TGGGCGCAGC ACGACATCAA 421 CTGTCTGATC CTGAAAGCGA ATGGTGCCAC CTCGGAGGAG CTGGCCGAGC TGAACCGCCT 481 GATGCAACAA CGCATTGACG AACCGAAGCG CATGGCGTAT CAGATCAATG ACCTGATGGT 541 TAGCGTTTTC CAGAAACAGA GCGAACTGGA GGCGAATCTG AAACTGATTC CGCGCAAGAA 601 ACAATTCGCA ATCATCAGCT TGAGCAAGGA AACGCTGAGC CAGATCCGTG ATGTTGAGAC 661 TAAAGCTAAG GCGGCGCTGG AAACCGCAGA CTCGGCAGAG GTTGAACAAA CGATTAAGTC 721 CGAGCTGAAG CAAAAACTGT CCGAAATTAA GGCTAATTAT CACGACGAGA AAGCGAACAT 781 CAAAGCATAC TGGTGGGAAA TGCTGGCGAA CATTAAGACC GAACTGAAAA CCGAGAAAAC 841 TGCCATTAAG CAGACCAACG ATTATGCAAA GCTGAAGGAG CTGAAGTGGA AGATTCACTT 901 CGAACCGTTG AACCTGAAGA AACAGTATCG TAGCTATTTC AAACAACTGA AAGCAAAATA 961 TAGCCTGAAG GACGGTAACC TTACCGAGTC GGAGCTGAGC CAGATCGAGG AGCTGCAGAA 1021 ACGCATCGTT AGCTTAAAGG ACTTTATCAA CCGTACCGCG AAGGAGGTTG CGGAGAAGCT 1081 GGAAATCACG AAGATCCTGC ACAAGCGTCC TGCGAATATC AGCGGTGGCC AGCAACAACG 1141 TGTCGCGATC GCCCGCGCTA TTGTCCGTCG CCCGAAGGTG CTGCTGATGG ATGAGCCTCT 1201 GAGCAACCTG GACGCGAAAC TGCGTGTGCA AACGCGCCAA TGGATCCGTA AGTTTCAGCA 1261 AGATCTGCAG ATTACGACGG TTTTCGTCAC CCATGACCAG GAGGAGGCCA TGAGCATTAG 1321 CGATACGATT GTCTGTATGA GCACGGGTAA AGTCCAGCAA ATTGGTAGCC CGAGCGAACT 1381 GTACCTGAAG CCTGCCAACG AATTTGTTGC AACCTTTCTT GGCAGCCCGG AGATGAACAT 1441 CGTGAACGCG ACCGTTAAAG CTGGTCAACT GCTGTGGAAT GAGAACCCGT TGGTTAAGAC 1501 CAAGTTCGAT CTGCCAGACG GCGCGATCCG CGTTGGTTTT CGTTATGATG AGGTGACCGC 1561 ACCGAAGAAT GACGGTAGCC CGGTTTTTAG CGGTACCCTG ATCAGCGTCG AGAATCTGGG 1621 CAAACACATG GTCGGTGTGG TTGAGAGCAA CGGTGTTCAG TTAAACGTGC GTCTGGAGCT 1681 GTCCCACCAG TTCGAGGTGG GTAATGCGGT TAAATTCACC ATTAAGCCGA ACGGTCTGCA 1741 TTTTTTCGAT CCGCAGACCA CCCAGCGTGT TGAGGTCAAA CATGTGTGAA GAGCTTAGAG 1801 ACCCCGATAG TTCCAGCTAG CATCGCTGAG GTGTCAATCG TCGGAGCCGC TGAGCAATAA 1861 CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCA TGGTCATAGC 1921 TGTTTCCTGA GAGCTTGGCA GGTGATGACA CACATTAACA AATTTCGTGA GGAGTCTCCA 1981 GAAGAATGCC ATTAATTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 2041 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 2101 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 2161 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 2221 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 2281 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 2341 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 2401 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 2461 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 2521 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 2581 TCAAGAAGGC CTACTATTAG CAACAACGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT 2641 CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC 2701 ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA 2761 ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA 2821 TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC 2881 TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGAAC CACGCTCACC GGCTCCAGAT 2941 TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA 3001 TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT 3061 AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT 3121 GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG 3181 TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC 3241 GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC 3301 GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG 3361 CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA 3421 ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA 3481 CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT 3541 TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG 3601 GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA 3661 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 3721 AAACAAATAG GGGTTCCGCG CACCTGCACC AGTCAGTAAA ACGACGGCCA GTAGTCAAAA 3781 GCCTCCGACC GGAGGCTTTT GACTTGGTCA GGTGGAGTGG GAG //