LOCUS Exported 3022 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..3022 /note="transcription terminator for bacteriophage T7 RNA polymerase" /label="T7 terminator" terminator 1060..1107 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 2955..2971 /note="common sequencing primer, one of multiple similar variants" /label="M13 for" terminator 2972..3003 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 29..988 /label="ugpE" /note="Probably part of a binding-protein-dependent transport system. Probably responsible for the translocation of the substrate across the membrane." /translation="MFKQSLIWYNRYVQKRKKGLDLTIKDRSWSNVLLGLIFKTVVLCFFGLMVIFPFYLMLVVALTSDEVVLNIREPILKSDGWHFENFRRVLEDGKYLNAIWINSLVTILSIILRLFFTVSMGYAFSLKKWKLKKLFWFIFLAVLILPESALLIGQYRVVVVANWNQPEKPAIILGLTMPFVASVFSGFMFRTAFEAIPPRIKESAFVDGCTGLRYFLKIAFPMVRSTTWTVSILTAFAAWNSYLWPLLLLTNRPDLNINLWVLAQGTDGNAGQSDEQIRVLLNLKMAAAILAILPMFIVYFLFRKRIMKAVGSRANTIKG" primer_bind complement(1112..1128) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(1195..1783) /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(1974..2834) /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" /label="bla" promoter complement(2835..2939) /label="bla promoter" /note="ampicillin resistance gene promoter" ORIGIN 1 AAACACGTGG CAAACATTCC GGTCTCAAAT GTTTAAACAG AGCCTGATCT GGTATAATCG 61 CTACGTTCAG AAACGCAAGA AAGGCTTAGA TCTGACCATC AAGGATCGTT CTTGGTCCAA 121 TGTGCTGTTA GGTCTGATCT TCAAAACCGT CGTGCTGTGC TTTTTTGGTC TGATGGTGAT 181 CTTCCCATTC TACCTGATGC TGGTCGTTGC GTTAACGTCC GATGAAGTCG TTCTGAACAT 241 CCGTGAACCG ATTCTGAAAA GCGACGGTTG GCACTTCGAA AACTTCCGTC GCGTTCTGGA 301 GGATGGTAAA TATCTGAACG CTATTTGGAT TAATTCTCTG GTTACGATCT TGTCAATCAT 361 TCTGCGTCTG TTCTTCACCG TCAGCATGGG TTATGCCTTC AGCCTGAAAA AATGGAAGCT 421 GAAAAAATTA TTCTGGTTCA TCTTTTTGGC GGTCCTGATT TTGCCAGAGT CAGCGCTGCT 481 GATCGGTCAA TATAGAGTCG TAGTAGTTGC TAACTGGAAT CAGCCGGAGA AGCCTGCTAT 541 TATCCTGGGC CTGACCATGC CGTTCGTCGC GAGCGTGTTT AGCGGTTTTA TGTTTCGTAC 601 GGCGTTCGAG GCAATCCCGC CGCGCATCAA AGAAAGCGCG TTCGTTGACG GTTGCACCGG 661 TCTGCGTTAC TTCCTGAAAA TCGCGTTTCC GATGGTACGC AGCACCACCT GGACGGTGAG 721 CATTCTGACG GCATTCGCGG CGTGGAATAG CTACCTGTGG CCGTTGCTGC TGTTGACGAA 781 CCGTCCGGAC CTGAATATCA ATCTGTGGGT CCTGGCACAG GGTACGGACG GCAATGCCGG 841 CCAATCCGAT GAACAGATCC GTGTTCTGCT GAATCTGAAG ATGGCCGCTG CGATTCTGGC 901 GATCCTGCCG ATGTTCATCG TCTACTTCCT GTTCCGCAAA CGCATCATGA AAGCAGTTGG 961 CAGCCGTGCA AACACCATTA AGGGCTGAAG AGCTTAGAGA CCCGGTGCTT TTGCGTGATA 1021 TTCGCTGAGG TGTCAATCGT CGGAGCCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC 1081 TCTAAACGGG TCTTGAGGGG TTTTTTGCAT GGTCATAGCT GTTTCCTGAG AGCTTGGCAG 1141 GTGATGACAC ACATTAACAA ATTTCGTGAG GAGTCTCCAG AAGAATGCCA TTAATTTCCA 1201 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 1261 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 1321 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 1381 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 1441 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 1501 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 1561 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 1621 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 1681 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 1741 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGGCC TACTATTAGC 1801 AACAACGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT 1861 TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA 1921 AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA 1981 TGCTTAATCA GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC 2041 TGACTCCCCG TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT 2101 GCAATGATAC CGCGAGAACC ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA 2161 GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT 2221 AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT 2281 GCCATTGCTA CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC 2341 GGTTCCCAAC GATCAAGGCG AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC 2401 TCCTTCGGTC CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT 2461 ATGGCAGCAC TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT 2521 GGTGAGTACT CAACCAAGTC ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC 2581 CCGGCGTCAA TACGGGATAA TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT 2641 GGAAAACGTT CTTCGGGGCG AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG 2701 ATGTAACCCA CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT 2761 GGGTGAGCAA AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA 2821 TGTTGAATAC TCATACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 2881 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 2941 ACCTGCACCA GTCAGTAAAA CGACGGCCAG TAGTCAAAAG CCTCCGACCG GAGGCTTTTG 3001 ACTTGGTCAG GTGGAGTGGG AG //