LOCUS       Exported     2872 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..2872
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      910..957
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     2805..2821
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      2822..2853
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..838
                     /label="yaaC"
                     /note="Catalyzes the phosphorylation of riboflavin to FMN followed by the adenylation of FMN to FAD."
                     /translation="MQQTLIIGAFDGLHKGHQLLAQAASGPVVALLIANIPSLQSDWLYEPKQRQVQLQQHFQSVVHSYDVIEHNISAQAFFDQIISPLRCQQLVVGADFCFGKDNQNADFLRRLFPNTTIIPKDSQTLSSSTIRQWLKQGQIEQANAVLLEPYFREGVVIRGNQQARFLGWPTANITLKPYMVPLRCGSYVITVTYHQTNYPGVGFISYKNDQLVCETHLIGFSGDLYGKQLRFTFNQFIRPQQKFSGVQALQKAISGDLKQAQKWFAQSTN"
     primer_bind     complement(962..978)
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 rev"
     rep_origin      complement(1045..1633)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(1824..2684)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(2685..2789)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 AAATAAGACG ACGACCCTCG GGTCTCAAAT GCAACAGACG CTGATTATTG GCGCATTTGA
       61 CGGTCTGCAC AAGGGTCACC AACTGCTGGC ACAGGCCGCA AGCGGTCCGG TCGTCGCCCT
      121 GTTGATCGCG AACATCCCAA GCCTGCAGAG CGACTGGCTG TACGAGCCGA AGCAGCGTCA
      181 GGTTCAGCTG CAACAACACT TTCAGAGCGT GGTTCATAGC TATGACGTCA TTGAGCATAA
      241 TATTAGCGCG CAAGCCTTCT TTGACCAAAT TATTAGCCCG CTGCGCTGCC AACAACTGGT
      301 TGTCGGCGCA GATTTCTGTT TTGGCAAGGA CAATCAGAAC GCGGACTTCC TGCGCCGTCT
      361 GTTTCCGAAT ACGACCATCA TTCCGAAAGA TAGCCAGACC TTGAGCAGCA GCACCATCCG
      421 CCAGTGGCTG AAACAAGGTC AAATTGAGCA AGCTAATGCC GTGTTGCTGG AACCGTATTT
      481 CCGTGAGGGT GTTGTCATCC GTGGTAACCA ACAGGCCCGT TTCTTGGGTT GGCCGACCGC
      541 GAATATCACT CTGAAACCGT ATATGGTCCC GCTGCGTTGC GGTTCCTATG TTATTACCGT
      601 TACGTACCAC CAAACCAATT ACCCGGGTGT GGGTTTTATT AGCTATAAGA ACGACCAACT
      661 GGTGTGTGAA ACGCATCTGA TCGGTTTTTC AGGTGACCTG TACGGCAAAC AACTGCGCTT
      721 CACCTTCAAC CAATTTATTC GTCCGCAACA GAAATTCAGC GGTGTCCAGG CGCTGCAGAA
      781 GGCGATCTCC GGCGATTTGA AACAGGCCCA GAAATGGTTC GCGCAAAGCA CCAACTGAAG
      841 AGCTTAGAGA CCGACCTTGG GATCGCTTAT GTCGCTGAGG TGTCAATCGT CGGAGCCGCT
      901 GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCAT
      961 GGTCATAGCT GTTTCCTGAG AGCTTGGCAG GTGATGACAC ACATTAACAA ATTTCGTGAG
     1021 GAGTCTCCAG AAGAATGCCA TTAATTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA
     1081 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT
     1141 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT
     1201 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT
     1261 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC
     1321 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT
     1381 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC
     1441 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT
     1501 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA
     1561 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA
     1621 AAAAGGATCT CAAGAAGGCC TACTATTAGC AACAACGATC CTTTGATCTT TTCTACGGGG
     1681 TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA
     1741 AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA
     1801 TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG
     1861 ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA
     1921 CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGAACC ACGCTCACCG
     1981 GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT
     2041 GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT
     2101 TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC
     2161 TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA
     2221 TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT
     2281 AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC
     2341 ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA
     2401 TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA TACCGCGCCA
     2461 CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA
     2521 AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT
     2581 TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG GCAAAATGCC
     2641 GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA
     2701 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT
     2761 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACCTGCACCA GTCAGTAAAA CGACGGCCAG
     2821 TAGTCAAAAG CCTCCGACCG GAGGCTTTTG ACTTGGTCAG GTGGAGTGGG AG
//