LOCUS       Exported     3196 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3196
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1234..1281
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3129..3145
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3146..3177
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..1162
                     /translation="MLWTRALILELKTNKQSRLLWLLAIPLLISLTLLTYGLVLFSSSGRIDHGDHFHLRERFVLTTEELVTFVVASVVFALTVALFGLGCWKLLQGPKVDRTNIKLANSNPAPQAVVLQADCDHFQVGDHCVFSAEKQHFKQQFKQDFLGKSKFSFRNELYRFCLIGVLISLNLALSMVEIPGIVLPWGSSIQFRFFNTAILFIAIRFVGLLSTSLIAIITPWIHLLLHPVHTPISTVFYMGNDLVVLWIFYFFYYHIFKAEVKQTTTVVNNKEFSQLVNTHKTKVAKALALIPVNLICGFIEGLGFYVGYFLILGKFGSVGHKIFYDSQANRDLINSANVIYFLLTTTTIFSLKYLFELLFFYSVEKGILNISRHFGLY"
                     /label="MPN160"
                     /note=""
     primer_bind     complement(1286..1302)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1369..1957)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2148..3008)
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
     promoter        complement(3009..3113)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 AAAGCACTCT TAGGCCTCTG GGTCTCAAAT GCTGTGGACG CGTGCCCTGA TCCTGGAGTT
       61 GAAAACCAAT AAGCAGAGCC GTCTGCTGTG GCTGCTTGCG ATTCCACTGC TGATTAGCCT
      121 GACGCTGCTG ACCTACGGTC TGGTCCTGTT TAGCAGCAGC GGTCGTATCG ATCACGGTGA
      181 TCACTTCCAT CTGCGCGAGC GCTTTGTCCT GACGACGGAG GAGCTGGTTA CCTTTGTTGT
      241 AGCGTCGGTT GTTTTCGCTC TGACTGTCGC ACTCTTCGGC CTGGGTTGCT GGAAGCTGCT
      301 CCAAGGTCCG AAAGTTGACC GCACTAATAT CAAGCTGGCG AATTCTAACC CGGCACCTCA
      361 AGCTGTTGTG CTGCAGGCAG ACTGTGATCA CTTCCAAGTG GGCGACCACT GTGTGTTCAG
      421 CGCGGAGAAA CAACACTTCA AGCAACAGTT CAAACAAGAT TTTCTGGGCA AATCTAAATT
      481 TTCTTTTCGT AACGAGCTGT ATCGCTTTTG CCTGATCGGT GTGCTGATTT CTCTGAACCT
      541 GGCGCTGAGC ATGGTCGAGA TTCCGGGTAT TGTCCTGCCG TGGGGTAGCA GCATTCAGTT
      601 CCGTTTCTTT AACACCGCAA TTTTGTTTAT CGCGATCCGC TTCGTTGGTC TGCTGAGCAC
      661 CAGCCTGATC GCCATCATTA CGCCGTGGAT CCATCTGCTG CTGCACCCGG TTCATACCCC
      721 AATCTCCACT GTATTCTATA TGGGTAATGA CCTGGTTGTG TTATGGATCT TCTACTTCTT
      781 TTACTATCAC ATTTTTAAGG CGGAAGTCAA GCAGACCACC ACGGTTGTTA ATAACAAGGA
      841 GTTTAGCCAG CTGGTTAATA CCCACAAAAC CAAAGTCGCC AAAGCACTGG CCCTGATTCC
      901 GGTCAATCTG ATTTGCGGTT TCATCGAAGG CCTGGGTTTT TACGTTGGTT ACTTTCTGAT
      961 TCTGGGCAAG TTCGGTAGCG TCGGCCATAA AATCTTCTAT GATAGCCAGG CAAACCGTGA
     1021 TCTGATTAAC AGCGCGAACG TTATCTACTT TCTGCTGACG ACCACGACCA TTTTCAGCCT
     1081 GAAATATCTG TTCGAATTGC TGTTCTTCTA TAGCGTGGAA AAAGGTATCC TGAACATCAG
     1141 CCGTCACTTC GGTCTGTACT GAAGAGCTTA GAGACCGGGA ATCTCGTCGG AAATCTCGCT
     1201 GAGGTGTCAA TCGTCGGAGC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA
     1261 CGGGTCTTGA GGGGTTTTTT GCATGGTCAT AGCTGTTTCC TGAGAGCTTG GCAGGTGATG
     1321 ACACACATTA ACAAATTTCG TGAGGAGTCT CCAGAAGAAT GCCATTAATT TCCATAGGCT
     1381 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC
     1441 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC
     1501 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC
     1561 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG
     1621 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA
     1681 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG
     1741 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA
     1801 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG
     1861 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG
     1921 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GGCCTACTAT TAGCAACAAC
     1981 GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG
     2041 ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA
     2101 AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGCTTA
     2161 ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC
     2221 CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG
     2281 ATACCGCGAG AACCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA
     2341 AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT
     2401 TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT
     2461 GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC
     2521 CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC
     2581 GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA
     2641 GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG
     2701 TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG
     2761 TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA
     2821 CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA
     2881 CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA
     2941 GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA
     3001 ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG
     3061 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACCTGC
     3121 ACCAGTCAGT AAAACGACGG CCAGTAGTCA AAAGCCTCCG ACCGGAGGCT TTTGACTTGG
     3181 TCAGGTGGAG TGGGAG
//