LOCUS       Exported     3025 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3025
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1063..1110
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     2958..2974
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      2975..3006
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..991
                     /translation="MKLNLRFPSYFLPVVAASAFLVSCATPNTYEVQRAALIQLVEEDERQNYIQKGSMGANAVMTAAKAETKTAEKTATSTKAASIELKKTDTDIKTTTTTENKSASGYKLDTLFGDYILWVVDHLSGLLFSPKTNNSTTTQKIQLITEDKMILDGGNLTVEKNHEHGHTHKNGETHEHDHDHHEGEEEVIVGRALSFANGLFLVVDLKEEKHEEKKEAKSEMSMNSKDMVMMTKTEMMSKEMKSEQKMEKKEEHEHPHKKLSLSTTAYKFGQSFNILEFTGAMHHKTAHNNETEFKNLGKKYGGMTEYTIVDFDFNPPKPTK"
                     /label="MPN162"
                     /note=""
     primer_bind     complement(1115..1131)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1198..1786)
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
                     /label="ori"
     CDS             complement(1977..2837)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(2838..2942)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 AAATAAGACG ACGACCCTCG GGTCTCAAAT GAAGCTGAAT CTGCGTTTTC CGAGCTACTT
       61 TCTGCCAGTG GTGGCAGCGA GCGCCTTCTT AGTTAGCTGC GCTACCCCGA ATACCTATGA
      121 AGTCCAGCGT GCGGCGCTGA TTCAACTCGT TGAGGAGGAT GAACGTCAAA ATTATATCCA
      181 GAAAGGCAGC ATGGGTGCGA ACGCGGTCAT GACCGCGGCC AAGGCGGAGA CTAAAACCGC
      241 GGAAAAGACG GCAACCAGCA CTAAGGCTGC TAGCATCGAG CTGAAAAAGA CCGATACTGA
      301 CATTAAGACC ACCACCACCA CCGAAAATAA GTCGGCATCT GGTTATAAAC TGGACACTTT
      361 ATTCGGTGAC TACATTCTGT GGGTGGTGGA TCACCTGTCC GGCTTGCTGT TTAGCCCTAA
      421 GACCAATAAC AGCACGACGA CCCAGAAAAT CCAGCTGATT ACCGAGGACA AGATGATTCT
      481 GGACGGCGGT AACCTGACGG TTGAGAAAAA CCATGAACAC GGTCACACCC ACAAAAATGG
      541 CGAAACCCAC GAACACGACC ATGATCACCA CGAAGGCGAG GAGGAAGTGA TCGTGGGTCG
      601 TGCCCTGAGC TTTGCTAATG GTCTGTTTCT GGTTGTCGAC CTGAAAGAAG AGAAGCACGA
      661 AGAGAAAAAA GAGGCCAAGA GCGAGATGAG CATGAACAGC AAGGATATGG TTATGATGAC
      721 TAAGACGGAG ATGATGTCTA AGGAGATGAA AAGCGAGCAA AAAATGGAGA AAAAAGAGGA
      781 GCATGAGCAC CCGCATAAAA AACTGAGCCT GAGCACCACC GCCTATAAAT TCGGCCAAAG
      841 CTTCAACATC CTGGAGTTTA CCGGCGCCAT GCATCACAAG ACGGCGCATA ATAACGAAAC
      901 CGAGTTCAAG AACTTAGGTA AAAAGTACGG CGGTATGACC GAGTATACCA TCGTTGACTT
      961 TGACTTCAAC CCTCCGAAGC CGACGAAGTG AAGAGCTTAG AGACCCCGGT TGTACCTATC
     1021 GAGTGCGCTG AGGTGTCAAT CGTCGGAGCC GCTGAGCAAT AACTAGCATA ACCCCTTGGG
     1081 GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CATGGTCATA GCTGTTTCCT GAGAGCTTGG
     1141 CAGGTGATGA CACACATTAA CAAATTTCGT GAGGAGTCTC CAGAAGAATG CCATTAATTT
     1201 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG
     1261 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC
     1321 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT
     1381 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA
     1441 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA
     1501 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA
     1561 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA
     1621 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT
     1681 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT
     1741 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG GCCTACTATT
     1801 AGCAACAACG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA
     1861 CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT
     1921 TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC
     1981 CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT
     2041 GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT
     2101 GCTGCAATGA TACCGCGAGA ACCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG
     2161 CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT
     2221 ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT
     2281 GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC
     2341 TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA AAAAGCGGTT
     2401 AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG
     2461 GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG
     2521 ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT
     2581 TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC
     2641 ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT GAGATCCAGT
     2701 TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT
     2761 TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG
     2821 AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT
     2881 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG
     2941 CGCACCTGCA CCAGTCAGTA AAACGACGGC CAGTAGTCAA AAGCCTCCGA CCGGAGGCTT
     3001 TTGACTTGGT CAGGTGGAGT GGGAG
//