LOCUS       Exported     2776 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..2776
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      814..861
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     2709..2725
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      2726..2757
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..742
                     /label="rplW"
                     /note="One of the early assembly proteins it binds 23S rRNA. One of the proteins that surrounds the polypeptide exit tunnel on the outside of the ribosome. Forms the main docking site for trigger factor binding to the ribosome (By similarity)."
                     /translation="MDVTNVLLKPVLTEKVYFNQMGETKKYVFVVNPKASKTRVKLAFELVYGIKPLKVNTLIRKPTTIRGGSRFPGLSKLEKLAVITLPKGIAISVTGEAPEKTDKPADKTTLKESTVKEIKDTKNSPEAVVKTAVEALQIKPTAAPVTTAPLQTVAVKVAKEVKEVKVEKPVKVEKPTKPAKVAKEAKTTKVAKETKAEKSVQTTKVAKETKTEKSAKTTKTTATKTTKTKTTKKEVKK"
     primer_bind     complement(866..882)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(949..1537)
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
                     /label="ori"
     CDS             complement(1728..2588)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(2589..2693)
                     /note="ampicillin resistance gene promoter"
                     /label="bla promoter"
ORIGIN
        1 AAGGCCCAGA AGGATACAAC GGTCTCAAAT GGACGTGACG AACGTTCTGC TGAAGCCGGT
       61 CCTGACCGAA AAAGTCTACT TCAACCAAAT GGGTGAAACC AAGAAATATG TGTTCGTGGT
      121 GAACCCGAAA GCATCTAAGA CCCGCGTGAA GTTAGCATTC GAGCTGGTCT ATGGCATCAA
      181 ACCGCTTAAA GTGAACACGC TGATTCGTAA ACCAACCACG ATTCGTGGTG GCAGCCGCTT
      241 CCCAGGTCTG AGCAAGCTGG AGAAACTGGC TGTCATCACG CTGCCGAAGG GCATTGCAAT
      301 TTCCGTCACG GGTGAGGCGC CGGAAAAGAC CGACAAGCCG GCGGATAAAA CCACGCTGAA
      361 AGAAAGCACC GTTAAAGAAA TCAAAGATAC GAAAAACTCC CCGGAGGCGG TTGTTAAGAC
      421 GGCGGTCGAG GCATTGCAGA TTAAACCAAC GGCGGCACCG GTCACGACCG CCCCGTTGCA
      481 GACCGTCGCC GTCAAGGTCG CGAAGGAGGT GAAGGAGGTT AAAGTTGAGA AACCGGTGAA
      541 AGTGGAGAAA CCGACGAAGC CTGCAAAAGT GGCCAAGGAG GCGAAAACTA CCAAAGTGGC
      601 AAAAGAAACC AAGGCCGAAA AAAGCGTCCA AACCACGAAG GTCGCCAAAG AAACCAAAAC
      661 CGAGAAGTCG GCTAAAACCA CGAAAACGAC TGCCACCAAA ACGACCAAGA CTAAAACCAC
      721 CAAGAAAGAA GTCAAAAAGT GAAGAGCTTA GAGACCGCTT GACGGAGCAT CTATCTCGCT
      781 GAGGTGTCAA TCGTCGGAGC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA
      841 CGGGTCTTGA GGGGTTTTTT GCATGGTCAT AGCTGTTTCC TGAGAGCTTG GCAGGTGATG
      901 ACACACATTA ACAAATTTCG TGAGGAGTCT CCAGAAGAAT GCCATTAATT TCCATAGGCT
      961 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC
     1021 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC
     1081 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC
     1141 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG
     1201 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA
     1261 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG
     1321 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA
     1381 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG
     1441 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG
     1501 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GGCCTACTAT TAGCAACAAC
     1561 GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG
     1621 ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA
     1681 AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGCTTA
     1741 ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC
     1801 CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG
     1861 ATACCGCGAG AACCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA
     1921 AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT
     1981 TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT
     2041 GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC
     2101 CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC
     2161 GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA
     2221 GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG
     2281 TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG
     2341 TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA
     2401 CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA
     2461 CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA
     2521 GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA
     2581 ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG
     2641 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACCTGC
     2701 ACCAGTCAGT AAAACGACGG CCAGTAGTCA AAAGCCTCCG ACCGGAGGCT TTTGACTTGG
     2761 TCAGGTGGAG TGGGAG
//