LOCUS       Exported     2482 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..2482
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      520..567
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     2415..2431
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      2432..2463
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..448
                     /label="rplP"
                     /note="Binds 23S rRNA and is also seen to make contacts with the A and possibly P site tRNAs."
                     /translation="MLQPKRTKYRKPHNVSYEGKAKGNSYVAFGEYGLVATKGNWIDARAIESARIAISKCLGKTGKMWIRIFPHMSKTKKPLEVRMGSGKGNPEFWVAVVKQGTVMFEVANIPESQMIKALTRAGHKLPVTWKILKREEVSA"
     primer_bind     complement(572..588)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(655..1243)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(1434..2294)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(2295..2399)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 AATCAGTTTC TTTGGCAGCC GGTCTCAAAT GCTGCAGCCG AAACGCACCA AATATCGTAA
       61 ACCGCACAAC GTCAGCTACG AGGGTAAAGC CAAGGGCAAT TCGTACGTGG CTTTTGGTGA
      121 ATATGGTCTG GTGGCGACGA AGGGTAACTG GATCGACGCG CGTGCGATCG AGAGCGCGCG
      181 TATTGCCATT AGCAAGTGCC TGGGCAAGAC GGGCAAAATG TGGATCCGTA TCTTCCCGCA
      241 CATGAGCAAG ACCAAGAAGC CGTTGGAGGT GCGTATGGGC AGCGGCAAAG GTAACCCGGA
      301 GTTCTGGGTT GCGGTTGTCA AGCAAGGCAC GGTCATGTTC GAAGTGGCAA ACATCCCGGA
      361 AAGCCAAATG ATTAAGGCAC TGACGCGTGC GGGTCATAAA CTGCCGGTGA CGTGGAAGAT
      421 CCTGAAACGT GAAGAAGTGA GCGCCTGAAG AGCTTAGAGA CCCCAGTATC GGCAGCTAAT
      481 CTCGCTGAGG TGTCAATCGT CGGAGCCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC
      541 TCTAAACGGG TCTTGAGGGG TTTTTTGCAT GGTCATAGCT GTTTCCTGAG AGCTTGGCAG
      601 GTGATGACAC ACATTAACAA ATTTCGTGAG GAGTCTCCAG AAGAATGCCA TTAATTTCCA
      661 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA
      721 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC
      781 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC
      841 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT
      901 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG
      961 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG
     1021 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA
     1081 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG
     1141 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT
     1201 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGGCC TACTATTAGC
     1261 AACAACGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT
     1321 TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA
     1381 AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA
     1441 TGCTTAATCA GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC
     1501 TGACTCCCCG TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT
     1561 GCAATGATAC CGCGAGAACC ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA
     1621 GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT
     1681 AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT
     1741 GCCATTGCTA CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC
     1801 GGTTCCCAAC GATCAAGGCG AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC
     1861 TCCTTCGGTC CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT
     1921 ATGGCAGCAC TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT
     1981 GGTGAGTACT CAACCAAGTC ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC
     2041 CCGGCGTCAA TACGGGATAA TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT
     2101 GGAAAACGTT CTTCGGGGCG AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG
     2161 ATGTAACCCA CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT
     2221 GGGTGAGCAA AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA
     2281 TGTTGAATAC TCATACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT
     2341 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC
     2401 ACCTGCACCA GTCAGTAAAA CGACGGCCAG TAGTCAAAAG CCTCCGACCG GAGGCTTTTG
     2461 ACTTGGTCAG GTGGAGTGGG AG
//