LOCUS       Exported     3496 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3496
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1534..1581
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3429..3445
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3446..3477
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..1462
                     /label="secY"
                     /note="The central subunit of the protein translocation channel SecYEG. Consists of two halves formed by TMs 1-5 and 6-10. These two domains form a lateral gate at the front which open onto the bilayer between TMs 2 and 7, and are clamped together by SecE at the back. The channel is closed by both a pore ring composed of hydrophobic SecY resides and a short helix (helix 2A) on the extracellular side of the membrane which forms a plug. The plug probably moves laterally to allow the channel to open. The ring and the pore may move independently."
                     /translation="MQAKPTTAVKQKQNFGQRLFTLLRNRDFMISFLITVVLLVLFRVLAIIPLPGIQVNQTGLDQNSNDFFSLFNLLGGGGLNQLSLFAVGISPYISAQIVMQLLSTDLIPPLSKLVNSGEVGRRKIEMITRIITLPFALVQSFAVIQIATNSGGGSSPITLKNNGSDFVAFYIIAMTAGTYLSVFLGDTISKKGIGNGITLLILSGILAQLPEGFIAAYSVLSGVVVTINATLTTAISFFIYFMAFVTLLFATTFITQETRKIPIQQSGQGLVTESSALPYLPIKVNSAGVIPVIFASSIMSIPVTIAQFQPQTESRWFVEDYLSLSKPTGIVLYGILVILFSFFYSYIQINPERLAKNFEKSGRFIPGIRPGKDTEKHIARVLVRINFIGAPFLTVIAIIPYIVSALIHLPNSLSLGGTGIIIIVTAVVEFMSALRSAATATNYQQLRRNLAIEVQKTAQQDKEEQLRAETPGIGNLW"
     primer_bind     complement(1586..1602)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1669..2257)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2448..3308)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3309..3413)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 AATGCAAAGC TATTAGCGCG GGTCTCAAAT GCAAGCTAAA CCGACGACCG CTGTCAAACA
       61 AAAGCAAAAC TTCGGCCAAC GTCTGTTCAC TCTCCTCCGT AACCGTGATT TCATGATTAG
      121 CTTTCTGATT ACCGTCGTTC TGTTGGTGTT GTTCCGTGTT CTGGCTATCA TTCCGCTGCC
      181 GGGTATTCAA GTGAATCAGA CCGGCTTAGA TCAGAATAGC AATGATTTTT TCAGCCTGTT
      241 TAATCTGCTG GGTGGTGGTG GTCTGAACCA GCTGAGCCTG TTCGCAGTGG GCATTAGCCC
      301 GTATATTAGC GCGCAAATTG TGATGCAACT GCTGAGCACC GATCTGATTC CGCCACTGTC
      361 CAAATTAGTC AACAGCGGTG AGGTGGGCCG CCGTAAGATC GAGATGATTA CGCGTATTAT
      421 TACCCTTCCG TTCGCTCTGG TGCAAAGCTT CGCGGTTATT CAGATCGCGA CCAACAGCGG
      481 TGGCGGCAGC AGCCCGATCA CTCTGAAAAA TAACGGTTCC GATTTCGTCG CGTTTTATAT
      541 TATCGCTATG ACCGCGGGTA CTTATCTGTC GGTCTTTCTG GGTGATACGA TTAGCAAAAA
      601 GGGCATTGGC AATGGTATCA CCTTGCTGAT TCTGTCTGGT ATTCTGGCGC AGCTGCCGGA
      661 AGGTTTCATT GCCGCGTATT CCGTCCTGAG CGGTGTTGTG GTGACCATCA ACGCCACGCT
      721 GACCACCGCC ATCAGCTTCT TTATCTACTT TATGGCATTC GTGACTCTGC TGTTCGCAAC
      781 CACGTTCATT ACCCAGGAAA CCCGCAAAAT CCCTATCCAG CAAAGCGGCC AAGGCCTGGT
      841 CACCGAAAGC TCCGCACTGC CGTATCTGCC GATTAAGGTC AACAGCGCTG GTGTCATCCC
      901 GGTTATCTTC GCGTCCAGCA TCATGAGCAT TCCAGTTACG ATTGCACAAT TTCAGCCTCA
      961 AACGGAGAGC CGTTGGTTTG TGGAGGACTA CTTAAGCCTG TCCAAACCAA CCGGTATCGT
     1021 CCTCTACGGT ATCCTGGTGA TCCTGTTCAG CTTCTTCTAC AGCTACATCC AGATCAATCC
     1081 AGAGCGTCTG GCGAAGAACT TTGAAAAGTC TGGCCGTTTT ATTCCGGGTA TCCGCCCGGG
     1141 CAAAGACACT GAGAAACATA TTGCCCGCGT TCTGGTGCGC ATTAACTTCA TCGGCGCGCC
     1201 GTTCCTCACC GTTATCGCCA TTATCCCGTA CATCGTGTCG GCACTGATCC ATCTGCCGAA
     1261 CAGCCTGTCC CTGGGTGGTA CCGGCATCAT CATCATCGTG ACCGCAGTCG TTGAATTTAT
     1321 GTCGGCACTG CGTAGCGCAG CAACCGCTAC CAACTACCAG CAACTGCGTC GTAATTTGGC
     1381 AATTGAGGTT CAAAAGACCG CGCAACAGGA TAAAGAGGAA CAACTGCGCG CAGAAACGCC
     1441 GGGTATCGGC AACCTGTGGT GAAGAGCTTA GAGACCGAGG TCAATCATGC CTCTCTCGCT
     1501 GAGGTGTCAA TCGTCGGAGC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA
     1561 CGGGTCTTGA GGGGTTTTTT GCATGGTCAT AGCTGTTTCC TGAGAGCTTG GCAGGTGATG
     1621 ACACACATTA ACAAATTTCG TGAGGAGTCT CCAGAAGAAT GCCATTAATT TCCATAGGCT
     1681 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC
     1741 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC
     1801 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC
     1861 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG
     1921 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA
     1981 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG
     2041 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA
     2101 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG
     2161 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG
     2221 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GGCCTACTAT TAGCAACAAC
     2281 GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG
     2341 ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA
     2401 AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGCTTA
     2461 ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC
     2521 CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG
     2581 ATACCGCGAG AACCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA
     2641 AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT
     2701 TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT
     2761 GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC
     2821 CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC
     2881 GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA
     2941 GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG
     3001 TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG
     3061 TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA
     3121 CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA
     3181 CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA
     3241 GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA
     3301 ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG
     3361 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACCTGC
     3421 ACCAGTCAGT AAAACGACGG CCAGTAGTCA AAAGCCTCCG ACCGGAGGCT TTTGACTTGG
     3481 TCAGGTGGAG TGGGAG
//