LOCUS Exported 3232 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..3232 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 1270..1317 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 3165..3181 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 3182..3213 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 29..1198 /note="Part of the binding-protein-dependent transport system for oligopeptides; probably responsible for the translocation of the substrate across the membrane." /translation="MFIVMTIVFFLVNSTGQTPLSATSSKDLEAVKTQLDAFGFNDPLIVRYGRYWQTLFSGSLGTYYSSPNQTIDQIVFGRVPNTLYVVLISFFIGSLLGIIFGMISGLFRGKLIDAVINVLVVLFVSIPSFVVGLGLLKAAGLFRLPPRFINFDDANFNFGNFLLASIIPILSLVFYTSAAFTYRVRNEVVEVMNQDYIKTARSKGLSTFAVALYHIFRNSIIPSVPLFVFGISGAFSGGFIIESLFGVQGVSRILIDSVQSNETNLVMFNIMFIQGIPLLASVFIELIYVLVDPRIRIASAGGVSLWTKLKFVYLRQAWLRKWRRINHTNSHNVLFNSPQHRQLLELKAIDYKHNTISLTEQQKTTLKIEPTANFVLLGTKCLKIITIHG" /label="oppB" primer_bind complement(1322..1338) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(1405..1993) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(2184..3044) /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" /label="bla" promoter complement(3045..3149) /note="ampicillin resistance gene promoter" /label="bla promoter" ORIGIN 1 AAGAATTACT GACCCCTCGG GGTCTCAAAT GTTCATTGTG ATGACCATCG TCTTTTTTCT 61 GGTGAACTCT ACTGGCCAAA CCCCACTGAG CGCCACCAGC AGCAAGGACC TGGAAGCCGT 121 CAAGACCCAA CTGGACGCCT TCGGCTTTAA TGACCCGCTG ATCGTTCGCT ACGGTCGCTA 181 CTGGCAGACC CTGTTCAGCG GCAGCCTGGG TACGTATTAC AGCAGCCCGA ATCAAACCAT 241 CGACCAAATT GTTTTTGGCC GCGTGCCGAA TACCTTGTAC GTGGTGCTGA TTTCGTTCTT 301 TATTGGCTCT CTGCTGGGCA TTATCTTCGG TATGATCAGC GGCCTGTTCC GCGGTAAGCT 361 GATTGATGCG GTGATCAATG TTTTGGTTGT TCTGTTCGTT AGCATCCCGA GCTTCGTTGT 421 GGGCCTCGGT CTGCTGAAGG CCGCCGGTCT GTTTCGTTTG CCACCGCGCT TTATTAACTT 481 TGACGACGCG AACTTTAATT TCGGTAACTT TCTGCTGGCT TCGATCATTC CGATCTTGAG 541 CCTGGTCTTT TATACGTCTG CTGCGTTTAC CTACCGCGTG CGTAATGAGG TTGTGGAGGT 601 TATGAACCAA GACTACATTA AGACCGCGCG TAGCAAAGGC CTGAGCACGT TTGCAGTGGC 661 GCTGTACCAT ATCTTCCGTA ACAGCATTAT TCCGTCTGTT CCGTTATTCG TTTTTGGCAT 721 TAGCGGCGCG TTCAGCGGCG GTTTCATCAT TGAATCATTG TTTGGCGTGC AAGGTGTCAG 781 CCGCATCCTG ATCGACAGCG TTCAAAGCAA CGAAACCAAT TTGGTGATGT TCAATATTAT 841 GTTCATTCAA GGCATCCCGC TGCTGGCTAG CGTTTTCATT GAGTTGATCT ATGTTCTGGT 901 GGACCCGCGT ATCCGTATCG CGAGCGCGGG TGGTGTCAGC CTGTGGACGA AGCTGAAGTT 961 CGTCTATCTG CGCCAGGCGT GGCTGCGCAA GTGGCGTCGT ATTAATCATA CGAACTCGCA 1021 CAACGTCCTG TTCAACAGCC CGCAGCACCG CCAGTTGCTG GAGCTGAAAG CGATCGATTA 1081 CAAACACAAC ACGATTAGCC TGACGGAGCA ACAAAAGACC ACGTTGAAGA TTGAGCCGAC 1141 CGCGAACTTT GTCCTGCTGG GCACCAAGTG TCTGAAGATT ATCACCATCC ACGGCTGAAG 1201 AGCTTAGAGA CCGCTGGGCA CTATGCTTTA CTCGCTGAGG TGTCAATCGT CGGAGCCGCT 1261 GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCAT 1321 GGTCATAGCT GTTTCCTGAG AGCTTGGCAG GTGATGACAC ACATTAACAA ATTTCGTGAG 1381 GAGTCTCCAG AAGAATGCCA TTAATTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 1441 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 1501 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 1561 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 1621 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 1681 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 1741 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 1801 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 1861 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 1921 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 1981 AAAAGGATCT CAAGAAGGCC TACTATTAGC AACAACGATC CTTTGATCTT TTCTACGGGG 2041 TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA 2101 AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA 2161 TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG 2221 ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA 2281 CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGAACC ACGCTCACCG 2341 GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT 2401 GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT 2461 TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC 2521 TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA 2581 TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT 2641 AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC 2701 ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA 2761 TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA TACCGCGCCA 2821 CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA 2881 AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT 2941 TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG GCAAAATGCC 3001 GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA 3061 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 3121 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACCTGCACCA GTCAGTAAAA CGACGGCCAG 3181 TAGTCAAAAG CCTCCGACCG GAGGCTTTTG ACTTGGTCAG GTGGAGTGGG AG //