LOCUS Exported 3334 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..3334 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 1372..1419 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 3267..3283 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 3284..3315 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 29..1300 /label="oppD" /note="Part of the binding-protein-dependent transport system for oligopeptides. Probably responsible for energy coupling to the transport system (By similarity)." /translation="MALKATNFFTEPEYKLQDDLILDIRDLHVSFKVKDGIMQAVRGVDLKVKKGSIVGIVGESGSGKSVCVKSIIGFNDGAKTTAKLMNFKNIDISKMKKHQWQYYRGTYVSYISQDPLFSLNPTMTIGRQVKEAIYVSCKRRYYQTKSDLKYDLQTERIDLDTYKEKLAQAKETYKAKTTKAAVHAKTLEILNFIGIDQAEKRLKAFPSEFSGGMRQRIVIAIAVATEPDLIIADEPTTALDVTIQAKALNLIKQLRDLLNITIIFISHNISLIANFCDFVYVMYAGRLVEKGLVEEIFTNPVHPYTWALMASIPEGTDKNAPLTSIPGYIPNMLSPPKGDAFAARNQFALAIDFEHQPPFFDVTETHKAATWLLHPQAPKVEMPADVKEKIAITRKALAIKMPSQPVKQPKSNGNKKTKTKSAR" primer_bind complement(1424..1440) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(1507..2095) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(2286..3146) /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" promoter complement(3147..3251) /label="bla promoter" /note="ampicillin resistance gene promoter" ORIGIN 1 ACAAGGAGTC GGCATATCAC GGTCTCAAAT GGCCCTGAAA GCAACCAACT TTTTTACCGA 61 GCCAGAGTAT AAGCTGCAAG ACGACCTGAT TCTGGATATC CGCGATCTGC ATGTCAGCTT 121 TAAAGTAAAG GACGGTATTA TGCAAGCGGT GCGTGGTGTT GACTTGAAAG TGAAAAAGGG 181 CTCCATTGTT GGCATCGTGG GTGAGAGCGG CTCTGGTAAG AGCGTTTGTG TTAAGTCTAT 241 TATTGGCTTC AATGACGGCG CCAAGACCAC TGCGAAGCTG ATGAATTTTA AAAACATCGA 301 TATTAGCAAA ATGAAAAAAC ACCAATGGCA ATACTACCGT GGTACCTATG TGAGCTACAT 361 CTCCCAGGAT CCGCTGTTCA GCCTGAACCC GACGATGACC ATCGGTCGTC AAGTTAAAGA 421 GGCTATTTAT GTCTCTTGTA AACGTCGTTA CTATCAGACC AAGAGCGATC TGAAGTACGA 481 TCTGCAGACC GAGCGTATTG ATCTGGACAC GTACAAAGAG AAACTGGCTC AAGCAAAGGA 541 AACGTACAAG GCCAAAACGA CGAAAGCGGC CGTGCACGCC AAAACCTTGG AGATTCTGAA 601 CTTCATCGGC ATTGATCAGG CTGAGAAACG CCTGAAGGCC TTTCCGAGCG AGTTCTCTGG 661 CGGCATGCGT CAACGTATTG TTATCGCGAT CGCCGTGGCA ACCGAGCCAG ATCTGATTAT 721 TGCAGATGAA CCTACTACCG CGCTGGACGT TACCATCCAA GCGAAAGCGC TGAATTTGAT 781 TAAACAGCTG CGTGATCTGC TGAATATCAC CATCATCTTC ATCAGCCACA ATATCAGCCT 841 GATTGCGAAT TTTTGCGATT TTGTCTATGT CATGTACGCG GGCCGTCTGG TCGAAAAAGG 901 CCTGGTGGAG GAGATCTTTA CGAACCCGGT CCATCCGTAC ACGTGGGCGC TGATGGCTAG 961 CATTCCGGAG GGTACCGACA AAAACGCGCC TTTGACGAGC ATTCCTGGCT ACATCCCGAA 1021 TATGCTGTCC CCGCCGAAAG GCGACGCGTT TGCAGCAAGA AACCAGTTCG CCCTGGCGAT 1081 TGACTTTGAG CACCAACCGC CATTCTTCGA TGTCACGGAA ACGCATAAAG CGGCGACGTG 1141 GCTGCTGCAT CCGCAGGCGC CGAAAGTCGA AATGCCGGCG GATGTGAAAG AGAAAATCGC 1201 TATTACTCGT AAAGCGCTGG CCATCAAAAT GCCATCCCAG CCTGTTAAAC AACCGAAATC 1261 CAACGGTAAT AAGAAAACCA AGACCAAGAG CGCCCGCTGA AGAGCTTAGA GACCCTTGAA 1321 ATCTTGTCGC ACGGCGCTGA GGTGTCAATC GTCGGAGCCG CTGAGCAATA ACTAGCATAA 1381 CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC ATGGTCATAG CTGTTTCCTG 1441 AGAGCTTGGC AGGTGATGAC ACACATTAAC AAATTTCGTG AGGAGTCTCC AGAAGAATGC 1501 CATTAATTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA 1561 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 1621 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC 1681 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT 1741 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC 1801 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC 1861 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 1921 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC 1981 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG 2041 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGG 2101 CCTACTATTA GCAACAACGA TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC 2161 GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC 2221 CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT 2281 GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA 2341 TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT 2401 GGCCCCAGTG CTGCAATGAT ACCGCGAGAA CCACGCTCAC CGGCTCCAGA TTTATCAGCA 2461 ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC 2521 ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG 2581 CGCAACGTTG TTGCCATTGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT 2641 TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA 2701 AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA 2761 TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC 2821 TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG 2881 AGTTGCTCTT GCCCGGCGTC AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA 2941 GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG 3001 AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC 3061 ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG 3121 GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 3181 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 3241 GGGGTTCCGC GCACCTGCAC CAGTCAGTAA AACGACGGCC AGTAGTCAAA AGCCTCCGAC 3301 CGGAGGCTTT TGACTTGGTC AGGTGGAGTG GGAG //