LOCUS       Exported     3499 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3499
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1537..1584
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3432..3448
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3449..3480
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..1465
                     /note=""
                     /translation="MMINFEAIIGIEVHVVLNTATKMFSPAPNQAQNATPNQFINAIDLGLPGTMPQVNEQAVQKALILADALNMQRVQPVLVFDRKHYFYQDLPKGFQITQQNFPIAQNGYVEIVENNKAQRIIIERFHLEEDTAKQHFVDGQILLDFNRCGAPLIEVVTAPVIKSAKQSKAYLQKLRQILIVNNISNAKLEDGSMRSDCNVSVRLKGQTAFGTKVEIKNINSLNNVEKAIDLEIARQVKALIKGEPVQQVTLTYDDKTNTNVFMRKKDNSVDYRYFIEPNIMSSNIDELLQKPNKAFNLTEFFTKLKEAGVNEQLNQLVVDDLTLFNAYTKINSVINSPQDTIRWLCIELMGQLNKLQKPLKDKTIDHLIILIQMVQKGTVNQKQAKQLIELMLDNGQDPQSLAKLHNLEQITDEKQLTQIIQQIFKENEGEILKNLDRVERIQKLIIGQVMQRTHNRANPQQVFIIVEKLLHDFSERAS"
                     /label="gatB"
     primer_bind     complement(1589..1605)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1672..2260)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2451..3311)
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
     promoter        complement(3312..3416)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 AATCAGTTTC TTTGGCAGCC GGTCTCAAAT GATGATCAAC TTCGAGGCGA TCATCGGTAT
       61 CGAGGTGCAC GTGGTGCTGA ATACGGCGAC GAAGATGTTT AGCCCGGCGC CGAACCAGGC
      121 TCAGAATGCC ACCCCTAACC AATTCATTAA TGCGATTGAT CTGGGTCTGC CGGGTACCAT
      181 GCCTCAGGTT AACGAGCAAG CCGTTCAAAA AGCATTGATT CTCGCCGACG CGCTGAATAT
      241 GCAACGTGTT CAACCGGTCT TGGTGTTCGA CCGCAAGCAC TATTTTTATC AAGATCTGCC
      301 GAAGGGTTTC CAGATTACGC AACAGAATTT CCCGATCGCG CAGAATGGCT ACGTCGAAAT
      361 CGTGGAAAAT AACAAAGCCC AACGTATCAT TATTGAGCGT TTCCACTTGG AAGAGGATAC
      421 CGCGAAACAG CACTTCGTGG ACGGTCAGAT CCTTCTTGAT TTTAACCGTT GCGGCGCTCC
      481 GCTGATTGAG GTGGTTACGG CACCGGTTAT TAAAAGCGCG AAACAAAGCA AAGCGTATCT
      541 GCAAAAGTTG CGTCAAATTC TGATTGTGAA CAACATTAGC AACGCGAAAC TGGAGGACGG
      601 CAGCATGCGT AGCGATTGTA ACGTGAGCGT GCGCTTAAAG GGCCAGACCG CGTTTGGCAC
      661 TAAAGTTGAG ATTAAAAACA TCAACAGCCT GAACAACGTT GAGAAGGCGA TCGACCTGGA
      721 AATTGCGCGT CAAGTTAAGG CTCTGATTAA AGGTGAACCG GTGCAACAAG TGACCTTAAC
      781 GTACGATGAT AAGACGAATA CCAATGTGTT CATGCGTAAG AAGGACAATT CTGTGGACTA
      841 TCGTTACTTT ATTGAGCCGA ACATCATGAG CAGCAATATC GACGAACTGC TGCAAAAACC
      901 GAACAAAGCA TTTAATTTGA CTGAGTTCTT CACGAAGCTG AAGGAAGCGG GTGTTAACGA
      961 GCAACTGAAC CAGCTGGTTG TGGACGATCT CACGCTGTTT AACGCATACA CGAAGATCAA
     1021 TAGCGTGATC AACAGCCCGC AAGATACCAT CCGTTGGCTG TGCATCGAGC TGATGGGTCA
     1081 ACTGAACAAA CTGCAAAAAC CGTTGAAGGA CAAAACGATC GATCATCTGA TTATTCTGAT
     1141 CCAAATGGTC CAGAAGGGTA CCGTTAACCA GAAGCAGGCG AAACAGTTGA TTGAGCTGAT
     1201 GTTGGACAAT GGTCAAGACC CTCAAAGCCT GGCTAAACTG CACAATCTGG AACAAATTAC
     1261 GGATGAGAAG CAACTGACCC AGATCATTCA GCAGATTTTC AAGGAAAACG AGGGTGAAAT
     1321 TCTGAAAAAT CTGGACCGTG TAGAACGTAT CCAAAAACTG ATCATTGGCC AGGTTATGCA
     1381 ACGTACGCAC AATAGAGCGA ACCCTCAACA GGTTTTCATC ATTGTGGAAA AACTGCTGCA
     1441 CGACTTCAGC GAGAGAGCTT CCTGAAGAGC TTAGAGACCC AGTCCTTGCC AATCGAAGTC
     1501 GCTGAGGTGT CAATCGTCGG AGCCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT
     1561 AAACGGGTCT TGAGGGGTTT TTTGCATGGT CATAGCTGTT TCCTGAGAGC TTGGCAGGTG
     1621 ATGACACACA TTAACAAATT TCGTGAGGAG TCTCCAGAAG AATGCCATTA ATTTCCATAG
     1681 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC
     1741 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT
     1801 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT
     1861 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG
     1921 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT
     1981 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT
     2041 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG
     2101 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA
     2161 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT
     2221 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGGCCTAC TATTAGCAAC
     2281 AACGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA
     2341 GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA
     2401 TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC
     2461 TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA
     2521 CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA
     2581 ATGATACCGC GAGAACCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC
     2641 GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT
     2701 TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC
     2761 ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT
     2821 TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC
     2881 TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG
     2941 GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT
     3001 GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG
     3061 GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA
     3121 AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG
     3181 TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG
     3241 TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT
     3301 TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC
     3361 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACC
     3421 TGCACCAGTC AGTAAAACGA CGGCCAGTAG TCAAAAGCCT CCGACCGGAG GCTTTTGACT
     3481 TGGTCAGGTG GAGTGGGAG
//