LOCUS       Exported     3079 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3079
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1117..1164
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3012..3028
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 for"
     terminator      3029..3060
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..1045
                     /note="Involved in the metabolism of galactose. Catalyzes the conversion of UDP-galactose (UDP-Gal) to UDP-glucose (UDP-Glc) through a mechanism involving the transient reduction of NAD (By similarity)."
                     /translation="MSETKSKVLVLGGLGYIGSCFIDQLLKQYPDVTVSVIDINHTSLALQLLPRQVNVHFVNLLDRAQLTDTIAQINPDVVFHFAAKTSVKESTEQPLTYFDHNLVGTLNLLHALKELQKPIQLFFSSTAAVFGSASTLPIPENLVLEETLASNPYGISKFLSEIVLQTLTRSPHFQVIALRYFNVAGASNPFGNFNKNTTLLIPNLIKAFMEKRTFFLYGDDYDTKDGSCIRDYIHVVDLCDAHLLAWKWLQANPKVRFESFNLGSGQGFSNWEVINTAQAIFAPEQLQLKIESRRAGDPPVLVVDCTKAKRLLNFQPTRSLHKMLSDETIFYRDFYNRL"
                     /label="galE"
     primer_bind     complement(1169..1185)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1252..1840)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2031..2891)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(2892..2996)
                     /note="ampicillin resistance gene promoter"
                     /label="bla promoter"
ORIGIN
        1 AAATAAGACG ACGACCCTCG GGTCTCAAAT GTCCGAAACG AAGTCTAAGG TTCTGGTGTT
       61 GGGTGGTTTG GGTTACATCG GTAGCTGCTT CATTGATCAG TTGCTTAAGC AATACCCAGA
      121 TGTTACGGTT TCTGTGATCG ACATCAATCA CACGAGCCTG GCGCTGCAGC TGCTGCCTCG
      181 CCAAGTGAAT GTTCACTTTG TTAATTTGTT GGACCGTGCG CAGCTGACCG ATACCATTGC
      241 GCAGATCAAT CCTGACGTTG TATTTCATTT TGCAGCTAAG ACCAGCGTTA AGGAGTCTAC
      301 GGAGCAACCG CTGACGTACT TCGACCATAA CCTTGTTGGC ACCCTGAACC TCCTGCACGC
      361 ACTGAAAGAG CTGCAGAAAC CGATCCAGCT GTTCTTTTCA AGCACGGCCG CGGTTTTTGG
      421 TAGCGCCAGC ACCCTGCCAA TTCCGGAGAA CCTGGTTTTG GAAGAAACCC TGGCCAGCAA
      481 TCCATACGGT ATTAGCAAAT TCCTGTCGGA GATTGTGCTG CAAACCTTGA CCCGTAGCCC
      541 GCACTTCCAG GTCATTGCGC TGCGCTATTT CAACGTCGCG GGTGCGTCGA ATCCGTTTGG
      601 TAATTTCAAT AAGAACACCA CGCTGCTGAT CCCTAATTTG ATCAAAGCAT TCATGGAGAA
      661 ACGTACCTTC TTTCTGTACG GTGATGATTA TGATACGAAA GATGGCAGCT GCATTCGCGA
      721 CTACATCCAT GTCGTTGACC TGTGTGATGC ACATCTGCTG GCCTGGAAAT GGCTGCAGGC
      781 TAATCCGAAG GTGCGTTTCG AGAGCTTCAA CTTGGGCAGC GGCCAGGGTT TTTCTAATTG
      841 GGAGGTTATC AACACCGCAC AAGCGATCTT TGCTCCGGAA CAACTGCAGC TGAAAATCGA
      901 ATCCCGTCGT GCGGGTGACC CGCCGGTGCT GGTCGTGGAT TGTACTAAGG CGAAGCGTCT
      961 GCTGAATTTC CAACCGACCC GCAGCCTGCA CAAAATGCTG AGCGACGAAA CCATTTTTTA
     1021 TCGTGATTTT TACAATCGTC TGTGAAGAGC TTAGAGACCC GCTAAGATCC GTAGGTCTGC
     1081 GCTGAGGTGT CAATCGTCGG AGCCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT
     1141 AAACGGGTCT TGAGGGGTTT TTTGCATGGT CATAGCTGTT TCCTGAGAGC TTGGCAGGTG
     1201 ATGACACACA TTAACAAATT TCGTGAGGAG TCTCCAGAAG AATGCCATTA ATTTCCATAG
     1261 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC
     1321 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT
     1381 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT
     1441 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG
     1501 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT
     1561 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT
     1621 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG
     1681 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA
     1741 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT
     1801 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGGCCTAC TATTAGCAAC
     1861 AACGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA
     1921 GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA
     1981 TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC
     2041 TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA
     2101 CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA
     2161 ATGATACCGC GAGAACCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC
     2221 GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT
     2281 TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC
     2341 ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT
     2401 TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC
     2461 TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG
     2521 GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT
     2581 GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG
     2641 GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA
     2701 AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG
     2761 TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG
     2821 TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT
     2881 TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC
     2941 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACC
     3001 TGCACCAGTC AGTAAAACGA CGGCCAGTAG TCAAAAGCCT CCGACCGGAG GCTTTTGACT
     3061 TGGTCAGGTG GAGTGGGAG
//