LOCUS       Exported     3616 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3616
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1654..1701
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
                     /label="T7 terminator"
     primer_bind     3549..3565
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 for"
     terminator      3566..3597
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..1582
                     /label="MPN259"
                     /note=""
                     /translation="MNALNYLKHRFLFSKDKFWYAPFKQKQRRSIYSTFSLIVLSFIVSFFLIVAIPGIKGGTFLEIFTRLFKDRINIENFARQIAIYTLAALAFSFCMSVGVFNIGISGQMMAGANFGFMMILKVFPESFRPAFGGQIITILLMILGSVTVAMVVAALKVFFKVNEVVSAIMLNWVIVLVSAYLVGTYIKPEKTDTSQFYSIELPDAFALYNFSDVQQKYGWLTSLVIAIAAAIFIAVLMKFTVFGHKLKSTGLSVTGSQAAGYSVKKYQFLSFVISGILSGLLAAVVYTASFEKQLTFSDVGDFGITSVPITGFDGIAIGLIALNSPARIVIVSTIISFVTIGAKPAGLNAATASLVLGIMMYFAAIYNLMIYIKPWRMIVKLNISKMNEAAYDEFQNEMAANLETLSFQRFLDKQKRKHDKERMVWFDTKRFEEYQKKKQATLQTFHENSSQNLLQYWKQQLLVADVKRLTFKWDFLTFKHQQKYILRWYKGKNKKQTALENEFASLNEAISQKLEEK"
     primer_bind     complement(1706..1722)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1789..2377)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2568..3428)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3429..3533)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 AAGGCCCAGA AGGATACAAC GGTCTCAAAT GAATGCCCTC AATTACCTGA AACACCGCTT
       61 CCTGTTCTCC AAGGATAAGT TTTGGTATGC TCCATTCAAG CAAAAGCAAC GCCGTAGCAT
      121 TTACAGCACC TTTAGCCTGA TCGTTCTGAG CTTCATCGTT AGCTTCTTCT TGATTGTGGC
      181 AATTCCGGGC ATCAAAGGTG GTACGTTCCT GGAGATCTTC ACCCGTCTGT TCAAGGATCG
      241 TATTAACATC GAGAACTTTG CGCGTCAGAT CGCAATCTAT ACGCTGGCTG CGCTGGCGTT
      301 TAGCTTCTGT ATGAGCGTTG GTGTCTTTAA CATTGGTATT AGCGGTCAGA TGATGGCGGG
      361 TGCGAATTTC GGTTTTATGA TGATTCTTAA AGTGTTCCCT GAGAGCTTCC GCCCGGCCTT
      421 TGGTGGCCAG ATTATTACCA TCCTGCTGAT GATCTTAGGT AGCGTTACGG TGGCCATGGT
      481 GGTGGCGGCG CTGAAAGTTT TCTTCAAGGT GAATGAAGTC GTGAGCGCGA TTATGCTGAA
      541 TTGGGTTATC GTACTGGTAA GCGCATACCT GGTGGGCACC TACATTAAAC CGGAGAAAAC
      601 CGATACCAGC CAGTTTTATT CCATTGAGCT GCCGGATGCC TTTGCCTTAT ATAACTTTAG
      661 CGACGTGCAA CAGAAGTACG GCTGGTTGAC CAGCCTGGTA ATTGCGATTG CGGCGGCTAT
      721 TTTCATTGCG GTACTGATGA AATTTACCGT CTTTGGTCAC AAACTGAAGT CGACCGGTCT
      781 GTCCGTTACG GGTTCTCAAG CAGCGGGCTA CAGCGTGAAG AAATATCAAT TTCTGAGCTT
      841 TGTGATTAGC GGCATTTTAT CTGGTTTGCT GGCTGCGGTT GTTTATACCG CTTCGTTTGA
      901 GAAGCAGCTG ACGTTTTCGG ATGTCGGTGA TTTTGGCATT ACCAGCGTTC CAATTACCGG
      961 TTTCGACGGT ATTGCAATCG GCCTGATCGC ATTGAACAGC CCGGCCCGTA TCGTTATTGT
     1021 TAGCACGATC ATTAGCTTCG TTACCATTGG TGCTAAACCG GCGGGTCTGA ATGCGGCAAC
     1081 GGCTTCTCTG GTGCTGGGTA TTATGATGTA TTTTGCCGCG ATCTATAACC TGATGATCTA
     1141 CATTAAACCG TGGCGTATGA TCGTTAAGCT GAACATTAGC AAAATGAACG AGGCAGCCTA
     1201 CGACGAATTC CAAAACGAGA TGGCCGCCAA CTTGGAGACT CTGTCCTTTC AGCGTTTTCT
     1261 GGACAAACAA AAGCGCAAAC ATGATAAGGA GCGTATGGTT TGGTTCGATA CTAAGCGTTT
     1321 CGAGGAGTAC CAGAAGAAAA AACAGGCAAC CCTGCAGACC TTTCACGAAA ACAGCAGCCA
     1381 AAATCTGCTG CAATATTGGA AACAACAACT GCTGGTGGCC GACGTTAAAC GCCTGACCTT
     1441 CAAGTGGGAT TTCCTGACCT TTAAGCATCA ACAGAAATAT ATCCTGCGTT GGTACAAAGG
     1501 CAAAAATAAG AAGCAAACCG CGTTGGAAAA TGAGTTCGCG AGCCTGAACG AAGCAATTTC
     1561 CCAGAAGCTG GAGGAGAAGT GAAGAGCTTA GAGACCCGCG GAAAATGGAA AACACTCGCT
     1621 GAGGTGTCAA TCGTCGGAGC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA
     1681 CGGGTCTTGA GGGGTTTTTT GCATGGTCAT AGCTGTTTCC TGAGAGCTTG GCAGGTGATG
     1741 ACACACATTA ACAAATTTCG TGAGGAGTCT CCAGAAGAAT GCCATTAATT TCCATAGGCT
     1801 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC
     1861 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC
     1921 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC
     1981 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG
     2041 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA
     2101 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG
     2161 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA
     2221 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG
     2281 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG
     2341 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GGCCTACTAT TAGCAACAAC
     2401 GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG
     2461 ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA
     2521 AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGCTTA
     2581 ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC
     2641 CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG
     2701 ATACCGCGAG AACCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA
     2761 AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT
     2821 TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT
     2881 GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC
     2941 CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC
     3001 GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA
     3061 GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG
     3121 TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG
     3181 TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA
     3241 CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA
     3301 CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA
     3361 GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA
     3421 ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG
     3481 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACCTGC
     3541 ACCAGTCAGT AAAACGACGG CCAGTAGTCA AAAGCCTCCG ACCGGAGGCT TTTGACTTGG
     3601 TCAGGTGGAG TGGGAG
//