LOCUS Exported 2908 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..2908 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 946..993 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 2841..2857 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 2858..2889 /note="bidirectional E. coli tonB-P14 transcription terminator" /label="tonB terminator" CDS 29..874 /label="MPN264" /note="" /translation="MIDLLGLDLDGTLLSRTRQINDPTKQALANLIQKKPSLKVMILTGRSLFSTLKYVQELNELCKKPLVEYFCCYGGAKLYQLNNNQPQEQYKFLIDSRQVKTVFEIVEQHKGLFLAYLDKPKAPYIILGANQFYAWLIKQFWYKQRCEYFKNDHLTDGILKINVYFACPLRLKKVYQIIKRQFQDTLNVVNFSKHLIEITHKDGNKGYAIEAIAKKQGLSLKRMAVIGDSLNDRSMFEKVQYSFAMSKSPDELKLLATEIGTKTNRFRFSSLVDLITEKIIN" primer_bind complement(998..1014) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(1081..1669) /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(1860..2720) /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" /label="bla" promoter complement(2721..2825) /label="bla promoter" /note="ampicillin resistance gene promoter" ORIGIN 1 AAATAAGACG ACGACCCTCG GGTCTCAAAT GATTGACTTA CTGGGCCTGG ACCTGGACGG 61 CACGTTGTTG AGCCGTACCC GTCAGATTAA TGATCCGACT AAACAGGCGC TGGCAAATCT 121 GATCCAGAAG AAGCCGAGCT TGAAGGTTAT GATTTTGACC GGCCGCAGCC TGTTCTCGAC 181 CCTGAAGTAT GTCCAGGAAC TGAATGAACT CTGTAAGAAG CCGCTTGTGG AGTATTTCTG 241 CTGTTACGGT GGCGCCAAGC TGTACCAGCT GAACAACAAC CAACCGCAGG AGCAGTATAA 301 ATTCCTGATT GATAGCCGCC AGGTCAAGAC TGTCTTTGAG ATTGTCGAGC AACACAAAGG 361 TTTGTTCCTG GCGTATCTGG ACAAGCCAAA GGCACCTTAC ATTATCCTGG GTGCTAACCA 421 GTTCTACGCT TGGCTGATCA AGCAATTTTG GTACAAGCAG CGCTGCGAGT ATTTCAAAAA 481 CGACCACTTG ACGGACGGCA TCTTGAAAAT CAATGTCTAT TTTGCCTGTC CGCTGCGTCT 541 GAAGAAAGTA TACCAAATTA TCAAACGTCA GTTCCAAGAT ACTCTGAATG TCGTCAATTT 601 CAGCAAACAC CTGATCGAAA TTACGCACAA GGACGGTAAT AAAGGCTACG CGATTGAGGC 661 GATCGCGAAA AAACAAGGCC TGAGCCTGAA GCGCATGGCG GTGATCGGTG ATAGCCTGAA 721 CGACCGCAGC ATGTTTGAAA AGGTTCAATA CAGCTTTGCC ATGAGCAAAA GCCCGGATGA 781 ACTGAAGCTG TTGGCAACGG AAATCGGTAC CAAAACGAAT CGTTTTCGCT TCAGCTCCCT 841 GGTGGACTTA ATTACGGAGA AAATCATCAA CTGAAGAGCT TAGAGACCGC GGTCGCTATA 901 CATACCTTCG CTGAGGTGTC AATCGTCGGA GCCGCTGAGC AATAACTAGC ATAACCCCTT 961 GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCATGGTC ATAGCTGTTT CCTGAGAGCT 1021 TGGCAGGTGA TGACACACAT TAACAAATTT CGTGAGGAGT CTCCAGAAGA ATGCCATTAA 1081 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 1141 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 1201 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 1261 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 1321 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 1381 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 1441 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 1501 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 1561 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 1621 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGGCCTACT 1681 ATTAGCAACA ACGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC 1741 TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA 1801 AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT 1861 TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA 1921 GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC 1981 AGTGCTGCAA TGATACCGCG AGAACCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC 2041 CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG 2101 TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC 2161 GTTGTTGCCA TTGCTACAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC 2221 AGCTCCGGTT CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG 2281 GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC 2341 ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT 2401 GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC 2461 TCTTGCCCGG CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC 2521 ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC 2581 AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC 2641 GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA 2701 CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 2761 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 2821 CCGCGCACCT GCACCAGTCA GTAAAACGAC GGCCAGTAGT CAAAAGCCTC CGACCGGAGG 2881 CTTTTGACTT GGTCAGGTGG AGTGGGAG //