LOCUS       Exported     3352 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3352
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1390..1437
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3285..3301
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3302..3333
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..1318
                     /label="prrB"
                     /note=""
                     /translation="MEIKTYKIKDICDIKRGRVISKLDIKKDPGVFPVYSAATNNDGEFGRINSYDFDGEYVTWTADGYGGAVFYRNGKFSITNLCGLLKVKNKEISSKYLAHILKLEAPKFTNRVFKNRPKLTHKTMAEIPIDFPPLKIQEKIATILDTFTELSAELSAELSAELSAELSAELSAELSAELSAELSAELSAELSAELSAELSAELSAELSAELRERRKQYAFYRDYLLNQENIRKIYGANIPFETFQIRDICEINRGREINEKYLRENPGEFPVYSSATTNGGLIGKINDYDFHGEYVTWTTGGAHAGNVFYRNEKFSCSQNCGLLEVKNKNKFSSKFLCFALKLQSKKFVNYASAIPVLTIKRIAEIELSFPPLEIQEKIADILFAFEKLCNDLTEGIPAEIELRKKQLDYYQNFLFNWVQNKKLESLKSL"
     primer_bind     complement(1442..1458)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1525..2113)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2304..3164)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3165..3269)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 ACAACGAGCA GACCGAATAG GGTCTCAAAT GGAAATCAAA ACCTACAAAA TTAAAGACAT
       61 CTGCGACATT AAACGCGGCC GCGTTATTTC CAAGCTGGAC ATTAAAAAAG ATCCGGGTGT
      121 TTTCCCGGTT TACAGCGCAG CAACCAACAA CGACGGCGAG TTTGGCCGTA TTAATTCTTA
      181 CGATTTTGAC GGCGAGTACG TGACCTGGAC TGCCGATGGT TACGGCGGCG CGGTGTTTTA
      241 CCGCAACGGC AAGTTTTCCA TCACCAACTT GTGCGGTCTG CTGAAAGTGA AAAACAAGGA
      301 AATCTCTAGC AAATATCTGG CCCATATTCT GAAGCTCGAA GCACCGAAAT TCACGAACCG
      361 CGTTTTCAAG AACCGTCCGA AGCTGACGCA CAAAACCATG GCGGAGATTC CGATCGATTT
      421 CCCGCCATTG AAAATCCAAG AAAAGATTGC AACCATCCTG GATACCTTCA CGGAGCTGAG
      481 CGCAGAGCTC AGCGCTGAAT TAAGCGCAGA ACTGAGCGCA GAGCTGAGCG CGGAGCTGTC
      541 GGCAGAGTTG AGCGCTGAGC TGTCAGCTGA ACTCTCTGCG GAGCTGTCCG CCGAGCTGAG
      601 CGCAGAATTG TCGGCGGAGC TGAGCGCAGA GTTGAGCGCA GAACTCAGCG CGGAGCTGCG
      661 TGAGCGTCGC AAGCAATACG CGTTTTATCG TGACTATCTG CTGAATCAGG AGAATATTCG
      721 CAAGATCTAC GGTGCGAATA TCCCGTTTGA AACCTTCCAA ATTCGTGATA TCTGTGAAAT
      781 TAACCGTGGC CGTGAGATCA ATGAGAAGTA TCTGCGTGAA AATCCGGGCG AATTTCCTGT
      841 CTACAGCTCC GCCACCACCA ACGGTGGTCT GATTGGTAAG ATCAATGACT ACGACTTCCA
      901 CGGCGAGTAT GTCACCTGGA CGACGGGCGG TGCTCATGCG GGCAATGTGT TCTATCGCAA
      961 CGAGAAATTT AGCTGTAGCC AGAATTGTGG TCTGCTGGAG GTTAAAAATA AAAACAAGTT
     1021 TAGCTCTAAA TTTCTGTGTT TTGCATTGAA ATTGCAGAGC AAGAAGTTCG TGAATTACGC
     1081 TTCCGCTATC CCGGTTTTGA CTATTAAGCG TATTGCTGAG ATCGAACTGA GCTTCCCACC
     1141 TCTGGAAATT CAAGAGAAAA TTGCGGACAT CCTGTTCGCG TTCGAGAAGC TGTGTAACGA
     1201 CCTGACTGAG GGCATCCCGG CGGAAATCGA ACTGCGTAAG AAGCAACTGG ATTACTATCA
     1261 AAACTTCCTG TTTAACTGGG TGCAGAACAA AAAATTGGAG AGCCTGAAGT CTCTGTGAAG
     1321 AGCTTAGAGA CCGTATGTCC CCTAAGCTCT GGCGCTGAGG TGTCAATCGT CGGAGCCGCT
     1381 GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCAT
     1441 GGTCATAGCT GTTTCCTGAG AGCTTGGCAG GTGATGACAC ACATTAACAA ATTTCGTGAG
     1501 GAGTCTCCAG AAGAATGCCA TTAATTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA
     1561 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT
     1621 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT
     1681 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT
     1741 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC
     1801 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT
     1861 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC
     1921 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT
     1981 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA
     2041 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA
     2101 AAAAGGATCT CAAGAAGGCC TACTATTAGC AACAACGATC CTTTGATCTT TTCTACGGGG
     2161 TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA
     2221 AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA
     2281 TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG
     2341 ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA
     2401 CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGAACC ACGCTCACCG
     2461 GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT
     2521 GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT
     2581 TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC
     2641 TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA
     2701 TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT
     2761 AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC
     2821 ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA
     2881 TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA TACCGCGCCA
     2941 CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA
     3001 AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT
     3061 TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG GCAAAATGCC
     3121 GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA
     3181 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT
     3241 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACCTGCACCA GTCAGTAAAA CGACGGCCAG
     3301 TAGTCAAAAG CCTCCGACCG GAGGCTTTTG ACTTGGTCAG GTGGAGTGGG AG
//