LOCUS Exported 2422 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..2422 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 460..507 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 2355..2371 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 2372..2403 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 29..388 /label="acpS" /note="Transfers the 4'-phosphopantetheine moiety from coenzyme A to a Ser of acyl-carrier-protein." /translation="MILGIGIDLVEIKRFEQLARQTDNCFAKRLLTSTEYAHYAKLRKDSEKSSFLAVHWSLKEAIYKAVNHIKPLFSQLEITKKNQRYNCQIDPKIELLLSVSYSSNNITAICLAQQTPWKN" primer_bind complement(512..528) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(595..1183) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(1374..2234) /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" promoter complement(2235..2339) /label="bla promoter" /note="ampicillin resistance gene promoter" ORIGIN 1 AAAGGGGCCG TCAATATCAG GGTCTCAAAT GATCTTGGGT ATCGGCATCG ACCTGGTTGA 61 GATCAAGCGC TTTGAACAGC TGGCGCGCCA GACGGATAAT TGTTTTGCGA AGCGTCTGCT 121 GACGTCTACC GAATATGCAC ACTACGCCAA ATTGCGCAAA GATAGCGAGA AAAGCAGCTT 181 CCTGGCCGTC CATTGGAGCT TGAAGGAGGC CATCTACAAA GCAGTTAACC ACATCAAGCC 241 TCTGTTTAGC CAACTGGAAA TCACCAAGAA AAACCAAAGA TACAACTGTC AGATCGATCC 301 GAAGATCGAG TTGTTGCTTT CCGTCAGCTA CAGCTCAAAC AACATTACCG CCATTTGTTT 361 GGCACAACAA ACGCCGTGGA AAAACTGAAG AGCTTAGAGA CCGCTAGTTG TCGTCATGGC 421 ATCGCTGAGG TGTCAATCGT CGGAGCCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC 481 TCTAAACGGG TCTTGAGGGG TTTTTTGCAT GGTCATAGCT GTTTCCTGAG AGCTTGGCAG 541 GTGATGACAC ACATTAACAA ATTTCGTGAG GAGTCTCCAG AAGAATGCCA TTAATTTCCA 601 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 661 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 721 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 781 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 841 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 901 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 961 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 1021 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 1081 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 1141 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGGCC TACTATTAGC 1201 AACAACGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT 1261 TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA 1321 AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA 1381 TGCTTAATCA GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC 1441 TGACTCCCCG TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT 1501 GCAATGATAC CGCGAGAACC ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA 1561 GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT 1621 AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT 1681 GCCATTGCTA CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC 1741 GGTTCCCAAC GATCAAGGCG AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC 1801 TCCTTCGGTC CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT 1861 ATGGCAGCAC TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT 1921 GGTGAGTACT CAACCAAGTC ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC 1981 CCGGCGTCAA TACGGGATAA TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT 2041 GGAAAACGTT CTTCGGGGCG AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG 2101 ATGTAACCCA CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT 2161 GGGTGAGCAA AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA 2221 TGTTGAATAC TCATACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 2281 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 2341 ACCTGCACCA GTCAGTAAAA CGACGGCCAG TAGTCAAAAG CCTCCGACCG GAGGCTTTTG 2401 ACTTGGTCAG GTGGAGTGGG AG //