LOCUS Exported 3049 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..3049 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 1087..1134 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 2982..2998 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 2999..3030 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 29..1015 /label="pfkA" /note="Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis." /translation="MSPKTTKKIAILTSGGDAPGMNATLVYLTRYATSSEIEVFFVKNGYYGLYHDELVPAHQLDLSNSLFSAGTVIGSKRFVEFKELKVREQAAQNLKKRQIDYLVVIGGDGSYMGAKLLSELGVNCYCLPGTIDNDINSSEFTIGFLTALESIKVNVQAVYHTTKSHERVAIVEVMGRHCGDLAIFGALATNADFVVTPSNKMDLKQLESAVKKILQHQNHCVVIVSENIYGFDGYPSLTAIKQHFDANNMKCNLVSLGHTQRGFAPTSLELVQISLMAQHTINLIGQNKVNQVIGNKANVPVNYDFDQAFNMPPVDRSALIAVINKNII" primer_bind complement(1139..1155) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(1222..1810) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(2001..2861) /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" promoter complement(2862..2966) /label="bla promoter" /note="ampicillin resistance gene promoter" ORIGIN 1 AAACCGGAGC CATACAGTAC GGTCTCAAAT GAGCCCTAAG ACCACGAAGA AAATCGCCAT 61 CCTCACCAGC GGCGGTGATG CGCCGGGTAT GAATGCCACC CTGGTGTACC TGACTCGTTA 121 CGCGACCAGC TCTGAAATTG AAGTGTTCTT TGTTAAGAAC GGCTATTATG GCCTGTATCA 181 TGACGAGCTG GTCCCGGCGC ACCAACTGGA TCTGTCTAAT AGCCTGTTCA GCGCTGGTAC 241 CGTTATTGGT AGCAAGCGCT TCGTCGAATT TAAGGAACTG AAGGTGCGCG AACAGGCAGC 301 GCAGAATCTG AAAAAGCGTC AGATTGACTA TCTGGTCGTT ATCGGTGGTG ACGGTTCCTA 361 TATGGGCGCT AAACTGCTGA GCGAGCTGGG TGTGAATTGC TACTGCCTGC CAGGTACCAT 421 CGACAACGAC ATCAACTCCA GCGAATTTAC GATTGGCTTC TTGACGGCAC TGGAATCCAT 481 CAAGGTGAAC GTTCAGGCGG TCTACCACAC CACCAAGAGC CACGAACGTG TGGCCATCGT 541 TGAGGTGATG GGTCGTCACT GTGGTGATTT GGCAATCTTT GGTGCATTGG CGACCAACGC 601 GGACTTTGTT GTGACGCCGT CCAATAAGAT GGACCTGAAA CAACTGGAGA GCGCGGTGAA 661 GAAGATCCTG CAACACCAGA ACCACTGCGT CGTGATCGTC AGCGAAAACA TCTACGGTTT 721 TGATGGCTAT CCGAGCCTGA CCGCTATCAA GCAGCACTTT GATGCAAATA ATATGAAATG 781 CAATCTGGTG AGCCTGGGCC ACACCCAACG TGGCTTCGCG CCGACGTCCC TGGAACTGGT 841 TCAGATCTCA CTGATGGCAC AACACACGAT TAACTTAATC GGCCAAAACA AGGTTAACCA 901 GGTGATTGGT AACAAGGCCA ATGTGCCGGT CAATTACGAT TTCGACCAGG CATTTAATAT 961 GCCGCCGGTT GATCGCAGCG CGCTGATCGC AGTTATTAAT AAGAACATCA TTTGAAGAGC 1021 TTAGAGACCC CGGTTGTACC TATCGAGTGC GCTGAGGTGT CAATCGTCGG AGCCGCTGAG 1081 CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCATGGT 1141 CATAGCTGTT TCCTGAGAGC TTGGCAGGTG ATGACACACA TTAACAAATT TCGTGAGGAG 1201 TCTCCAGAAG AATGCCATTA ATTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 1261 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 1321 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 1381 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 1441 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 1501 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 1561 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 1621 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 1681 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 1741 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 1801 AGGATCTCAA GAAGGCCTAC TATTAGCAAC AACGATCCTT TGATCTTTTC TACGGGGTCT 1861 GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG 1921 ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT 1981 GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC 2041 TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG 2101 GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGAACCACG CTCACCGGCT 2161 CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA 2221 ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG 2281 CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG 2341 TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC 2401 CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG 2461 TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG 2521 CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG 2581 TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT 2641 AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG 2701 ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA 2761 GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA 2821 AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT 2881 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 2941 AAAAATAAAC AAATAGGGGT TCCGCGCACC TGCACCAGTC AGTAAAACGA CGGCCAGTAG 3001 TCAAAAGCCT CCGACCGGAG GCTTTTGACT TGGTCAGGTG GAGTGGGAG //