LOCUS       Exported     3397 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3397
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1435..1482
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
                     /label="T7 terminator"
     primer_bind     3330..3346
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3347..3378
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
                     /label="tonB terminator"
     CDS             29..1363
                     /label="tig"
                     /note="Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation. Functions as a peptidyl-prolyl cis-trans isomerase (By similarity)."
                     /translation="MKQYKLVNTTQKEKTLCLEIAIDTKLWKETQQKQTQDLTKNMKIKGFRKGKVPPTLAKDYLDRAELLQRSAQAVIDAIFQPLQQEAVIADNENVIEDFPTIDFKTINENDCVILFDFDLVPQFEPPDYKHIKDLSPIVPLKDEEFNKELHNIEKNKGKLVDVSDKALANNDIAVIDFVGKVDGKVLESATAKQYELTIGSNSFIDGFESGLIGMKVGDKRQLKLKFPKDYHAEELKGKPVEFDIELKAIKQLEITPMDETNFKEYLPAQYQGFNSLKEFKTYFHKLVSAKKLEITLQENSVKIRQFFLANTTLPYIPDSLIKLESDRLLRAQKDQAEQYKIPFERLLAASKLSLEQLQQRNIKEARDNVTFALVMKRIADVEKIKVDNKKIHSEIESIIDVEYPFVNAELKKQMFHNMEQQKDFVESIILNRLTTTKIIEYSTH"
     primer_bind     complement(1487..1503)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1570..2158)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2349..3209)
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
     promoter        complement(3210..3314)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 AAGGCGCTCG GATAATACTC GGTCTCAAAT GAAGCAGTAC AAGCTGGTGA ACACGACTCA
       61 AAAAGAAAAG ACTCTGTGTT TGGAAATCGC AATTGACACC AAACTGTGGA AAGAAACCCA
      121 GCAAAAACAG ACCCAAGATC TGACGAAAAA TATGAAGATC AAGGGCTTTC GTAAGGGTAA
      181 GGTGCCGCCG ACCCTGGCGA AAGATTACCT GGACCGCGCC GAACTTCTGC AGCGCAGCGC
      241 GCAGGCAGTG ATCGATGCCA TTTTTCAACC TCTGCAACAG GAGGCGGTTA TCGCAGATAA
      301 CGAAAACGTG ATTGAGGATT TTCCGACTAT CGACTTCAAA ACCATCAATG AGAATGACTG
      361 CGTTATTCTG TTCGACTTTG ACCTCGTTCC GCAATTCGAG CCACCTGATT ACAAACACAT
      421 TAAGGATCTC TCGCCGATCG TGCCACTGAA AGATGAAGAG TTCAATAAAG AGCTGCACAA
      481 CATTGAGAAA AATAAAGGTA AACTCGTTGA CGTGAGCGAC AAAGCACTGG CAAACAACGA
      541 CATTGCGGTT ATCGACTTCG TGGGTAAAGT TGATGGCAAA GTTCTGGAGA GCGCGACCGC
      601 AAAACAGTAT GAGCTGACCA TTGGTTCCAA TTCTTTTATT GACGGTTTCG AAAGCGGTCT
      661 GATCGGTATG AAGGTGGGCG ATAAACGCCA ACTGAAACTG AAATTTCCGA AGGACTACCA
      721 CGCGGAAGAA CTGAAAGGTA AGCCGGTCGA GTTCGATATC GAACTGAAAG CTATTAAACA
      781 GCTGGAGATC ACGCCGATGG ACGAGACTAA CTTCAAAGAG TATCTGCCGG CGCAATATCA
      841 AGGTTTCAAC AGCCTGAAGG AATTCAAGAC CTACTTCCAC AAATTGGTGA GCGCGAAAAA
      901 ACTGGAGATC ACCTTGCAAG AGAACAGCGT TAAAATCCGT CAGTTTTTCC TGGCGAACAC
      961 CACGCTGCCT TATATCCCGG ACAGCCTGAT CAAACTGGAA AGCGATCGTC TGTTGCGTGC
     1021 GCAAAAAGAT CAAGCGGAAC AGTACAAAAT TCCGTTTGAG CGCTTGCTGG CTGCGAGCAA
     1081 GCTGAGCCTG GAGCAACTGC AGCAGCGTAA CATCAAGGAG GCTCGTGACA ATGTCACCTT
     1141 CGCACTGGTG ATGAAGCGTA TCGCGGACGT CGAAAAGATC AAGGTGGACA ACAAGAAAAT
     1201 TCACAGCGAG ATTGAGTCTA TCATTGACGT GGAGTACCCG TTCGTGAACG CGGAGCTGAA
     1261 AAAACAGATG TTCCACAATA TGGAACAGCA GAAGGACTTC GTTGAGAGCA TTATTTTGAA
     1321 TCGCCTGACC ACGACCAAGA TCATTGAGTA TTCCACGCAT TGAAGAGCTT AGAGACCGTG
     1381 ATATGCCGAC TCCTTGTCGC TGAGGTGTCA ATCGTCGGAG CCGCTGAGCA ATAACTAGCA
     1441 TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCATGGTCA TAGCTGTTTC
     1501 CTGAGAGCTT GGCAGGTGAT GACACACATT AACAAATTTC GTGAGGAGTC TCCAGAAGAA
     1561 TGCCATTAAT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG
     1621 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC
     1681 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC
     1741 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT
     1801 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT
     1861 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC
     1921 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA
     1981 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA
     2041 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG
     2101 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA
     2161 AGGCCTACTA TTAGCAACAA CGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG
     2221 AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG
     2281 ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG
     2341 TCTGACAGTT ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT
     2401 TCATCCATAG TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA
     2461 TCTGGCCCCA GTGCTGCAAT GATACCGCGA GAACCACGCT CACCGGCTCC AGATTTATCA
     2521 GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC
     2581 TCCATCCAGT CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT
     2641 TTGCGCAACG TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG
     2701 GCTTCATTCA GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC
     2761 AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG
     2821 TTATCACTCA TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA
     2881 TGCTTTTCTG TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA
     2941 CCGAGTTGCT CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA
     3001 AAAGTGCTCA TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG
     3061 TTGAGATCCA GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT
     3121 TTCACCAGCG TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA
     3181 AGGGCGACAC GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT
     3241 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA
     3301 ATAGGGGTTC CGCGCACCTG CACCAGTCAG TAAAACGACG GCCAGTAGTC AAAAGCCTCC
     3361 GACCGGAGGC TTTTGACTTG GTCAGGTGGA GTGGGAG
//