LOCUS       Exported     3043 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3043
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1081..1128
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     2976..2992
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      2993..3024
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
                     /label="tonB terminator"
     CDS             29..1009
                     /note=""
                     /translation="MSVTPIVEVKHLEKEFGFWRKNRILKDVNFAIMPGEFHAFIGQNGAGKTTTIKCLISSYQRFKDEINIDGISNKNAKSKGVISYIPEYAVFPKHLNTHEYLYTLGKLSGCSLQTIKEKVDYWLARFQIEHLRFKKPNDFSSGQKKKVLLIQALFNDPKLLIMDEPTANLDPKTRNEFMDVCYELNVRNKMAVFVSSHILAELENYCDSLTVIHEGEILFNGKTKNIAKDNFGYRLKVNNSDSLRKWLKAQKIAYKYFPATDDFQVDLKQKQSNKFAVELYQQPDFEVFIFARQGNSLQNIYNNLIEQYEYDQVQRAVAIREQDEAV"
                     /label="bcrA"
     primer_bind     complement(1133..1149)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1216..1804)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(1995..2855)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(2856..2960)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 AAGGCGCTCG GATAATACTC GGTCTCAAAT GAGCGTTACC CCGATTGTTG AAGTGAAACA
       61 TCTGGAAAAG GAATTCGGCT TTTGGCGTAA GAACCGCATT CTGAAGGATG TCAATTTCGC
      121 GATTATGCCG GGTGAATTCC ACGCATTCAT CGGTCAAAAC GGCGCTGGCA AGACCACGAC
      181 GATTAAATGT CTGATTAGCA GCTACCAACG TTTCAAGGAT GAAATTAACA TTGACGGCAT
      241 CAGCAATAAA AACGCAAAGA GCAAGGGCGT TATTAGCTAC ATTCCGGAGT ACGCAGTTTT
      301 CCCGAAACAT CTGAATACGC ATGAATACCT GTACACGCTG GGCAAGCTGA GCGGCTGCTC
      361 CCTGCAGACC ATCAAGGAAA AAGTTGATTA CTGGCTGGCG CGCTTCCAAA TTGAGCATCT
      421 GCGTTTTAAG AAACCGAATG ACTTCTCCTC CGGCCAGAAA AAGAAAGTGC TGCTGATTCA
      481 GGCGCTGTTT AATGACCCGA AACTGTTAAT CATGGATGAA CCGACCGCAA ACCTGGATCC
      541 TAAGACCCGT AACGAGTTTA TGGACGTGTG CTATGAGCTG AATGTCCGTA ACAAGATGGC
      601 GGTGTTCGTC AGCTCTCACA TTCTCGCAGA GCTGGAGAAT TACTGCGATT CTTTAACTGT
      661 CATTCACGAG GGCGAGATTC TGTTCAACGG TAAGACCAAA AATATCGCAA AGGACAACTT
      721 CGGTTATCGC TTAAAGGTTA ATAATTCTGA CTCTCTGCGT AAGTGGCTGA AGGCCCAAAA
      781 GATCGCATAT AAATACTTTC CGGCGACGGA CGACTTCCAG GTCGATCTGA AGCAAAAACA
      841 AAGCAACAAG TTCGCAGTGG AGCTGTACCA ACAACCGGAC TTTGAGGTGT TCATTTTCGC
      901 GCGTCAAGGT AATTCTCTGC AGAACATCTA CAACAACCTG ATTGAGCAGT ACGAGTATGA
      961 CCAAGTGCAG CGCGCAGTGG CGATCCGCGA ACAGGATGAG GCGGTGTGAA GAGCTTAGAG
     1021 ACCCCCCGTG AATGCTATGT TCTCGCTGAG GTGTCAATCG TCGGAGCCGC TGAGCAATAA
     1081 CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCA TGGTCATAGC
     1141 TGTTTCCTGA GAGCTTGGCA GGTGATGACA CACATTAACA AATTTCGTGA GGAGTCTCCA
     1201 GAAGAATGCC ATTAATTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG
     1261 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG
     1321 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT
     1381 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT
     1441 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG
     1501 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT
     1561 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT
     1621 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT
     1681 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC
     1741 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC
     1801 TCAAGAAGGC CTACTATTAG CAACAACGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT
     1861 CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC
     1921 ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA
     1981 ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA
     2041 TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC
     2101 TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGAAC CACGCTCACC GGCTCCAGAT
     2161 TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA
     2221 TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT
     2281 AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT
     2341 GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG
     2401 TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC
     2461 GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC
     2521 GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG
     2581 CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA
     2641 ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA
     2701 CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT
     2761 TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG
     2821 GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA
     2881 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT
     2941 AAACAAATAG GGGTTCCGCG CACCTGCACC AGTCAGTAAA ACGACGGCCA GTAGTCAAAA
     3001 GCCTCCGACC GGAGGCTTTT GACTTGGTCA GGTGGAGTGG GAG
//