LOCUS Exported 3043 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..3043 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 1081..1128 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 2976..2992 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 2993..3024 /note="bidirectional E. coli tonB-P14 transcription terminator" /label="tonB terminator" CDS 29..1009 /note="" /translation="MSVTPIVEVKHLEKEFGFWRKNRILKDVNFAIMPGEFHAFIGQNGAGKTTTIKCLISSYQRFKDEINIDGISNKNAKSKGVISYIPEYAVFPKHLNTHEYLYTLGKLSGCSLQTIKEKVDYWLARFQIEHLRFKKPNDFSSGQKKKVLLIQALFNDPKLLIMDEPTANLDPKTRNEFMDVCYELNVRNKMAVFVSSHILAELENYCDSLTVIHEGEILFNGKTKNIAKDNFGYRLKVNNSDSLRKWLKAQKIAYKYFPATDDFQVDLKQKQSNKFAVELYQQPDFEVFIFARQGNSLQNIYNNLIEQYEYDQVQRAVAIREQDEAV" /label="bcrA" primer_bind complement(1133..1149) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(1216..1804) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(1995..2855) /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" promoter complement(2856..2960) /label="bla promoter" /note="ampicillin resistance gene promoter" ORIGIN 1 AAGGCGCTCG GATAATACTC GGTCTCAAAT GAGCGTTACC CCGATTGTTG AAGTGAAACA 61 TCTGGAAAAG GAATTCGGCT TTTGGCGTAA GAACCGCATT CTGAAGGATG TCAATTTCGC 121 GATTATGCCG GGTGAATTCC ACGCATTCAT CGGTCAAAAC GGCGCTGGCA AGACCACGAC 181 GATTAAATGT CTGATTAGCA GCTACCAACG TTTCAAGGAT GAAATTAACA TTGACGGCAT 241 CAGCAATAAA AACGCAAAGA GCAAGGGCGT TATTAGCTAC ATTCCGGAGT ACGCAGTTTT 301 CCCGAAACAT CTGAATACGC ATGAATACCT GTACACGCTG GGCAAGCTGA GCGGCTGCTC 361 CCTGCAGACC ATCAAGGAAA AAGTTGATTA CTGGCTGGCG CGCTTCCAAA TTGAGCATCT 421 GCGTTTTAAG AAACCGAATG ACTTCTCCTC CGGCCAGAAA AAGAAAGTGC TGCTGATTCA 481 GGCGCTGTTT AATGACCCGA AACTGTTAAT CATGGATGAA CCGACCGCAA ACCTGGATCC 541 TAAGACCCGT AACGAGTTTA TGGACGTGTG CTATGAGCTG AATGTCCGTA ACAAGATGGC 601 GGTGTTCGTC AGCTCTCACA TTCTCGCAGA GCTGGAGAAT TACTGCGATT CTTTAACTGT 661 CATTCACGAG GGCGAGATTC TGTTCAACGG TAAGACCAAA AATATCGCAA AGGACAACTT 721 CGGTTATCGC TTAAAGGTTA ATAATTCTGA CTCTCTGCGT AAGTGGCTGA AGGCCCAAAA 781 GATCGCATAT AAATACTTTC CGGCGACGGA CGACTTCCAG GTCGATCTGA AGCAAAAACA 841 AAGCAACAAG TTCGCAGTGG AGCTGTACCA ACAACCGGAC TTTGAGGTGT TCATTTTCGC 901 GCGTCAAGGT AATTCTCTGC AGAACATCTA CAACAACCTG ATTGAGCAGT ACGAGTATGA 961 CCAAGTGCAG CGCGCAGTGG CGATCCGCGA ACAGGATGAG GCGGTGTGAA GAGCTTAGAG 1021 ACCCCCCGTG AATGCTATGT TCTCGCTGAG GTGTCAATCG TCGGAGCCGC TGAGCAATAA 1081 CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCA TGGTCATAGC 1141 TGTTTCCTGA GAGCTTGGCA GGTGATGACA CACATTAACA AATTTCGTGA GGAGTCTCCA 1201 GAAGAATGCC ATTAATTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 1261 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 1321 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 1381 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 1441 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 1501 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 1561 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 1621 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 1681 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 1741 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 1801 TCAAGAAGGC CTACTATTAG CAACAACGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT 1861 CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC 1921 ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA 1981 ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA 2041 TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC 2101 TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGAAC CACGCTCACC GGCTCCAGAT 2161 TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA 2221 TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT 2281 AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT 2341 GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG 2401 TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC 2461 GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC 2521 GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG 2581 CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA 2641 ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA 2701 CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT 2761 TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG 2821 GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA 2881 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 2941 AAACAAATAG GGGTTCCGCG CACCTGCACC AGTCAGTAAA ACGACGGCCA GTAGTCAAAA 3001 GCCTCCGACC GGAGGCTTTT GACTTGGTCA GGTGGAGTGG GAG //