LOCUS       Exported     3928 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3928
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1966..2013
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3861..3877
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3878..3909
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..1894
                     /translation="MVKKELEMYNLYTFQIDLDKKLLFEKADNQQNYSKIRARYFKNSARNQQAVFLNKNLIKNTFNKALLNFSDFLSGSGVESIFKQVIDDQPEVLNYLKQVKKEDSCDGHSEASQLVFNVVINPKNTLANFFEELTIYLHFNEENNTVVGSFSLKWNIKRADLFSETKNIAINNLIHTFCKNNLNEVSFIQIIKCFAKTLINKQGQIVLESCAFKQKWQNIVEQKYPFSTIHKNLKIVNSDFFDAFFVILLLICHLNNNLLWLCEKTEHFEWKLNSKISNFKEDNTEVYLSKMLLFLKDWYFENQAVTNEDIEKVDEVEDIGKLVEKYSANQPQKLSSNSTVYVFEPDKKQCFLKNDDFFNTNEAKLLFLITMQPNVFGLDDTAIANDLNLREIGDFFKEIDFTDSDVLNDFQQQKETLLVRRTFNQLLFMNSNTDVLSIVNNKFKSAIHNIVWTITYSKAIMLKAFDYSKIFEQNRTNDPSLLRSNLNSINRLRYLSEYFRTASVKYDQLYTKVKEYMQLDQFLVDMINQVNHEDEIFGKYKERVYLSLGIVTAVVFGIIEFFNCVWTVLTVSQQTAEKSLADPRNTVIIGIGTILVLTLLITILTFMTRRLYLFEFNKKHK"
                     /label="MPN337"
                     /note=""
     primer_bind     complement(2018..2034)
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 rev"
     rep_origin      complement(2101..2689)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2880..3740)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3741..3845)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 AAACACGTGG CAAACATTCC GGTCTCAAAT GGTGAAGAAG GAACTCGAGA TGTACAATCT
       61 GTACACCTTC CAAATTGACC TGGACAAGAA GCTCCTGTTT GAAAAAGCTG ACAACCAGCA
      121 AAACTATAGC AAAATCCGTG CGCGCTATTT TAAGAACAGC GCACGCAACC AGCAGGCCGT
      181 TTTTCTGAAT AAAAACCTGA TTAAGAATAC TTTCAATAAG GCACTCCTGA ACTTCAGCGA
      241 TTTCCTGAGC GGCTCTGGCG TTGAAAGCAT TTTCAAACAG GTGATCGACG ACCAGCCAGA
      301 AGTTTTGAAC TACCTGAAAC AGGTTAAAAA GGAGGACTCA TGTGATGGCC ACAGCGAGGC
      361 GAGCCAACTG GTTTTCAACG TCGTCATCAA CCCGAAGAAC ACTCTGGCGA ACTTTTTCGA
      421 GGAGCTTACT ATCTATCTGC ACTTCAACGA GGAAAATAAC ACTGTGGTGG GTAGCTTCAG
      481 CCTGAAGTGG AATATCAAAC GCGCAGACCT GTTTAGCGAA ACCAAAAATA TCGCTATCAA
      541 CAACCTGATT CACACGTTCT GCAAAAACAA TTTGAACGAA GTCTCCTTCA TTCAGATCAT
      601 TAAGTGCTTC GCAAAGACGT TGATCAACAA GCAAGGTCAA ATTGTCCTGG AATCTTGCGC
      661 CTTCAAGCAA AAGTGGCAAA ATATCGTTGA GCAGAAATAT CCGTTCAGCA CCATCCACAA
      721 AAACCTGAAG ATCGTCAATA GCGACTTTTT TGACGCGTTC TTCGTTATTC TGCTGCTCAT
      781 CTGTCACTTG AACAACAATC TGCTGTGGCT GTGTGAAAAA ACCGAGCACT TCGAGTGGAA
      841 GCTGAATTCC AAAATCTCGA ACTTCAAGGA GGATAACACC GAGGTCTACC TGAGCAAAAT
      901 GTTGCTGTTC CTGAAAGATT GGTATTTCGA GAATCAGGCT GTTACCAATG AAGATATTGA
      961 AAAAGTGGAT GAAGTTGAAG ATATTGGTAA ACTGGTGGAG AAATACAGCG CGAACCAACC
     1021 GCAAAAGCTG AGCTCCAACT CCACCGTTTA TGTGTTCGAA CCGGACAAAA AACAATGCTT
     1081 TTTGAAAAAT GATGACTTTT TTAACACGAA CGAAGCGAAA CTGTTGTTCC TGATTACGAT
     1141 GCAACCGAAC GTTTTTGGTC TGGACGACAC CGCGATCGCA AACGATCTGA ATTTGCGTGA
     1201 GATCGGTGAC TTTTTTAAGG AGATCGACTT TACCGATTCT GATGTTCTGA ATGATTTTCA
     1261 ACAGCAGAAA GAAACCCTTC TGGTGCGTCG CACGTTCAAC CAACTGCTGT TCATGAATAG
     1321 CAATACTGAC GTTTTGTCTA TCGTTAATAA CAAGTTCAAG TCTGCTATCC ACAACATCGT
     1381 TTGGACCATT ACCTATAGCA AAGCGATCAT GTTGAAAGCG TTCGATTACA GCAAGATTTT
     1441 CGAGCAAAAC CGCACGAATG ACCCGTCCCT GCTGCGCAGC AATCTGAATA GCATCAATCG
     1501 CTTGCGCTAT CTGAGCGAGT ATTTTCGTAC CGCCTCCGTC AAGTACGATC AGCTGTACAC
     1561 CAAAGTTAAA GAGTACATGC AGCTGGACCA GTTCCTGGTT GACATGATTA ATCAGGTTAA
     1621 CCACGAGGAT GAAATCTTCG GCAAATACAA GGAACGTGTT TACCTGAGCC TGGGTATCGT
     1681 CACTGCTGTT GTTTTTGGCA TCATTGAGTT CTTTAATTGC GTTTGGACGG TTTTGACCGT
     1741 TAGCCAACAA ACGGCAGAGA AAAGCTTAGC GGATCCGCGC AATACCGTGA TCATCGGTAT
     1801 TGGCACCATC CTGGTGCTGA CCCTGCTGAT TACCATCCTG ACCTTCATGA CCCGTCGCCT
     1861 GTATCTGTTC GAATTCAATA AAAAGCATAA GTGAAGAGCT TAGAGACCGC CCGGCAATTC
     1921 TTATAGCTCG CTGAGGTGTC AATCGTCGGA GCCGCTGAGC AATAACTAGC ATAACCCCTT
     1981 GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCATGGTC ATAGCTGTTT CCTGAGAGCT
     2041 TGGCAGGTGA TGACACACAT TAACAAATTT CGTGAGGAGT CTCCAGAAGA ATGCCATTAA
     2101 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG
     2161 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG
     2221 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG
     2281 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC
     2341 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA
     2401 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG
     2461 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC
     2521 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC
     2581 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG
     2641 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGGCCTACT
     2701 ATTAGCAACA ACGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC
     2761 TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA
     2821 AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT
     2881 TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA
     2941 GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC
     3001 AGTGCTGCAA TGATACCGCG AGAACCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC
     3061 CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG
     3121 TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC
     3181 GTTGTTGCCA TTGCTACAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC
     3241 AGCTCCGGTT CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG
     3301 GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC
     3361 ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT
     3421 GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC
     3481 TCTTGCCCGG CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC
     3541 ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC
     3601 AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC
     3661 GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA
     3721 CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT
     3781 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT
     3841 CCGCGCACCT GCACCAGTCA GTAAAACGAC GGCCAGTAGT CAAAAGCCTC CGACCGGAGG
     3901 CTTTTGACTT GGTCAGGTGG AGTGGGAG
//