LOCUS       Exported     3961 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3961
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
                     /label="T7 terminator"
     terminator      1999..2046
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3894..3910
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 for"
     terminator      3911..3942
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..1927
                     /note=""
                     /translation="MEFNKLQLSHCISFYISDVSEVFFESINQHPSRDFVNNILQKIKSTLSEEELEKLNTIEEVTKDEKILIMLNHVLKKIVSKTGSSSCDLFQIVKHDRFNEPVYIQSVNAFENNLINNEFAERRYDYLIEINKHSYLRKYVNAIRISFFLDLRAQILSGSFTLDVINKQLEHQNKEELFQAIYLRSLIKHFISNQLYPISLNSFIFGDKNRENKTVENDKLSVLKNNWNQIFFSKYFDFVAKNKEERVVDNNCDELFYATMNTLLIMLIIIEELRVYFNSKEPALILKLLDNKVSLREDPDQNPETDLHELIKFAEKNYLEKEKTSRWHKKRVKSLEELLEEIKQINLETKNESLAYPDEIVELELDNVHNFVSTKQVFRHQLDLQTLHGIVINPERYGIGMWSNHFVDWEEFKDLIEQITDAENGSDLYGFEKDLDESICQVNKKYLTFISSDSSSFLIIKNDQTKVISNYVWAQLYFETRRWIINDIEYDLYEKGFDKSHFASNIALLESLNFNWLDPFYGLTSIKEIMQKIDSKSNLKTSIAEMVAKFKHEQRISKKDNERVLMIFAYVAAAVVGFINFFSMVFTILTVSDLNAGLTPANIVVIAIASLLALFLIVIAVLFRFRWKYIKH"
                     /label="MPN338"
     primer_bind     complement(2051..2067)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(2134..2722)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2913..3773)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3774..3878)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 AATGCAAAGC TATTAGCGCG GGTCTCAAAT GGAATTCAAT AAACTGCAGC TGAGCCACTG
       61 CATTTCGTTC TACATTTCGG ATGTTAGCGA AGTTTTCTTT GAAAGCATTA ATCAGCACCC
      121 ATCTCGTGAC TTTGTCAACA ATATTCTGCA GAAAATCAAA AGCACCCTGA GCGAAGAGGA
      181 GCTGGAGAAA CTGAACACCA TTGAAGAAGT CACCAAGGAT GAGAAAATCT TGATTATGCT
      241 GAATCACGTT CTCAAGAAAA TCGTTAGCAA GACGGGTAGC AGCAGCTGCG ACCTGTTCCA
      301 GATCGTGAAG CACGACCGTT TTAATGAACC GGTGTACATC CAGTCAGTCA ATGCCTTTGA
      361 GAACAACCTG ATTAACAATG AATTCGCAGA ACGTCGTTAC GACTACTTGA TCGAAATTAA
      421 CAAGCACAGC TATCTGCGTA AGTATGTTAA TGCAATTCGT ATTAGCTTCT TTCTGGATCT
      481 GCGTGCCCAG ATCCTGTCCG GTAGCTTCAC CTTAGATGTC ATCAATAAGC AATTGGAACA
      541 TCAAAATAAA GAGGAGCTGT TCCAGGCAAT TTATCTGCGT AGCCTGATTA AACATTTCAT
      601 TAGCAATCAA CTGTACCCGA TTAGCCTGAA CTCCTTCATT TTCGGTGACA AGAACCGTGA
      661 GAACAAAACC GTTGAGAACG ATAAGCTGAG CGTTCTGAAA AATAACTGGA ATCAGATCTT
      721 TTTTAGCAAG TACTTCGATT TTGTGGCGAA AAACAAGGAG GAACGTGTCG TCGATAACAA
      781 CTGCGACGAG CTGTTTTACG CGACTATGAA CACGTTGCTG ATCATGCTGA TCATTATCGA
      841 GGAGCTGCGT GTCTACTTTA ACAGCAAAGA ACCGGCGCTG ATTCTGAAAT TGTTGGATAA
      901 CAAAGTGAGC CTGCGCGAGG ACCCGGATCA AAACCCGGAA ACTGATCTGC ACGAACTGAT
      961 TAAGTTTGCG GAAAAGAACT ACCTGGAAAA GGAAAAAACG AGCCGTTGGC ACAAGAAGCG
     1021 TGTTAAAAGC TTGGAAGAAC TGCTGGAGGA GATTAAGCAG ATTAACCTGG AAACGAAAAA
     1081 TGAAAGCCTG GCATATCCGG ATGAAATTGT GGAACTGGAA CTGGACAATG TGCACAACTT
     1141 CGTGAGCACC AAACAGGTTT TCCGTCACCA GCTGGACTTG CAGACGTTGC ATGGCATCGT
     1201 GATCAACCCA GAACGCTACG GTATTGGTAT GTGGAGCAAT CACTTTGTTG ACTGGGAGGA
     1261 GTTTAAGGAT CTGATCGAAC AAATCACCGA CGCGGAAAAC GGTTCCGACC TGTACGGTTT
     1321 TGAGAAAGAC TTGGACGAGA GCATCTGTCA AGTTAACAAG AAATACTTGA CCTTCATCAG
     1381 CAGCGATAGC AGCTCCTTCC TGATTATCAA GAATGACCAG ACCAAGGTTA TTTCAAACTA
     1441 CGTCTGGGCA CAGCTGTACT TCGAAACCCG TCGTTGGATT ATCAATGACA TTGAATACGA
     1501 CCTGTATGAA AAGGGTTTCG ACAAGAGCCA CTTCGCGAGC AATATTGCGC TGCTGGAATC
     1561 TCTGAATTTT AACTGGCTGG ACCCTTTCTA TGGTCTGACC AGCATCAAAG AGATCATGCA
     1621 AAAGATCGAT AGCAAAAGCA ACCTGAAAAC CTCCATTGCG GAGATGGTTG CCAAGTTCAA
     1681 ACATGAGCAG CGTATCAGCA AGAAAGACAA CGAGCGTGTG CTGATGATCT TTGCGTACGT
     1741 CGCGGCTGCT GTCGTCGGTT TTATCAATTT CTTTTCCATG GTTTTTACCA TCCTGACTGT
     1801 TAGCGACCTG AACGCGGGTC TGACGCCGGC AAATATCGTC GTTATTGCGA TTGCATCCTT
     1861 GCTGGCTTTG TTTCTGATTG TTATCGCGGT CCTGTTTCGT TTCCGTTGGA AGTACATTAA
     1921 GCATTGAAGA GCTTAGAGAC CGTGTTCTAT GTCCCCATCG TCGCTGAGGT GTCAATCGTC
     1981 GGAGCCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT
     2041 TTTTTGCATG GTCATAGCTG TTTCCTGAGA GCTTGGCAGG TGATGACACA CATTAACAAA
     2101 TTTCGTGAGG AGTCTCCAGA AGAATGCCAT TAATTTCCAT AGGCTCCGCC CCCCTGACGA
     2161 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA
     2221 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC
     2281 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG
     2341 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC
     2401 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG
     2461 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT
     2521 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT
     2581 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG
     2641 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC
     2701 GCGCAGAAAA AAAGGATCTC AAGAAGGCCT ACTATTAGCA ACAACGATCC TTTGATCTTT
     2761 TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA
     2821 TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC
     2881 TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT
     2941 ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA
     3001 ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGAACCA
     3061 CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA
     3121 AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA
     3181 GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG
     3241 GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA
     3301 GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT
     3361 GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT
     3421 CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA
     3481 TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT
     3541 ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA
     3601 AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC
     3661 AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG
     3721 CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC
     3781 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT
     3841 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CCTGCACCAG TCAGTAAAAC
     3901 GACGGCCAGT AGTCAAAAGC CTCCGACCGG AGGCTTTTGA CTTGGTCAGG TGGAGTGGGA
     3961 G
//