LOCUS Exported 2713 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..2713 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 751..798 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 2646..2662 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 2663..2694 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 29..679 /label="MPN344" /note="" /translation="MPKIISKRKFNILRKTKEEIFSQIVYTKKRNGLHKAAKEYYFKDKDFTIIEDQQDRPDKPEELDTPDIPKPPKPPKGPDQPEEPGQPGGPDDPNSGNKKMPKPDEFVTHRQLQEFKKDLLVELHEIFPTKPELKRVEEKVDVLFELQKAQGEQIRIQGEQIKELKVEQKAQGETLQLILQTLQKMNDRLDKIEGKMDKMESRMDKMETRLDKLESK" primer_bind complement(803..819) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(886..1474) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(1665..2525) /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" /label="bla" promoter complement(2526..2630) /label="bla promoter" /note="ampicillin resistance gene promoter" ORIGIN 1 AAGGCGCTCG GATAATACTC GGTCTCAAAT GCCGAAAATC ATTTCCAAAC GTAAATTTAA 61 TATTCTCCGT AAGACCAAGG AGGAAATTTT TAGCCAGATC GTCTACACCA AGAAACGCAA 121 TGGCCTGCAC AAAGCTGCGA AGGAGTATTA CTTCAAAGAC AAGGACTTTA CTATCATTGA 181 GGATCAGCAG GACCGCCCGG ACAAGCCAGA GGAGCTGGAT ACCCCGGACA TTCCTAAACC 241 GCCGAAACCG CCGAAAGGTC CAGATCAACC GGAGGAGCCG GGCCAACCGG GTGGCCCGGA 301 CGACCCGAAT AGCGGTAATA AAAAGATGCC GAAACCGGAT GAGTTTGTGA CCCACCGTCA 361 GCTTCAAGAG TTTAAAAAGG ACCTGTTGGT GGAGCTGCAT GAGATCTTTC CGACGAAACC 421 GGAGCTGAAA CGCGTGGAGG AGAAGGTGGA TGTTCTGTTC GAGCTGCAGA AGGCGCAGGG 481 TGAGCAAATT CGTATCCAAG GCGAGCAGAT TAAAGAGCTG AAAGTTGAGC AGAAGGCACA 541 GGGTGAAACG CTGCAGCTGA TCCTGCAAAC CCTGCAGAAG ATGAACGACC GCCTGGATAA 601 AATCGAAGGC AAAATGGACA AAATGGAAAG CCGCATGGAC AAAATGGAAA CCCGCCTGGA 661 TAAGCTGGAA AGCAAGTGAA GAGCTTAGAG ACCGTGTCGC TCGCAATCCT AATCGCTGAG 721 GTGTCAATCG TCGGAGCCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG 781 GTCTTGAGGG GTTTTTTGCA TGGTCATAGC TGTTTCCTGA GAGCTTGGCA GGTGATGACA 841 CACATTAACA AATTTCGTGA GGAGTCTCCA GAAGAATGCC ATTAATTTCC ATAGGCTCCG 901 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 961 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 1021 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 1081 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 1141 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 1201 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 1261 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 1321 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 1381 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 1441 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGGC CTACTATTAG CAACAACGAT 1501 CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT 1561 TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT 1621 TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC 1681 AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC 1741 GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA 1801 CCGCGAGAAC CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG 1861 GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC 1921 CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT 1981 ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA 2041 CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT 2101 CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA 2161 CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC 2221 TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA 2281 ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT 2341 TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC 2401 ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA 2461 AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA 2521 CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 2581 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACCTGCACC 2641 AGTCAGTAAA ACGACGGCCA GTAGTCAAAA GCCTCCGACC GGAGGCTTTT GACTTGGTCA 2701 GGTGGAGTGG GAG //