LOCUS Exported 2839 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..2839 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 877..924 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 2772..2788 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 2789..2820 /note="bidirectional E. coli tonB-P14 transcription terminator" /label="tonB terminator" CDS 29..805 /label="MPN359" /note="" /translation="MNYSYSFKEYIERFAKKVNSIDSEYFEFSSYIERMRTVFGLLIALICFSNVLCFLFIATWFSTKGFGQHYRALIFTLFIPFVTSLLANIIFINLNRAFREYFKISSKSRSFLVICAFSSLPIVNIWLMLWWVAMIKRFTSNYAFAIFDKYNGLTSGVFIFDFADNVNFEGKLVSFDNTKDTNRDFVHFYSEAKLKRDKITLQTNPIPHERMYVNRMYYQQKLSMGANQNSPSTAFANLKRYVEHKQQKIIKIKQFILT" primer_bind complement(929..945) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(1012..1600) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(1791..2651) /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" promoter complement(2652..2756) /label="bla promoter" /note="ampicillin resistance gene promoter" ORIGIN 1 AAGACGATCC GAGCCATTAC GGTCTCAAAT GAACTACAGC TACAGCTTCA AAGAGTACAT 61 CGAGCGCTTC GCTAAGAAAG TCAATAGCAT TGATAGCGAG TATTTCGAAT TCAGCAGCTA 121 CATCGAGCGT ATGCGTACCG TGTTCGGTCT GCTGATCGCT CTGATCTGCT TCAGCAATGT 181 GCTGTGCTTC CTGTTCATTG CGACGTGGTT TAGCACGAAA GGTTTTGGCC AGCATTACCG 241 TGCCCTTATT TTTACCCTGT TCATTCCGTT CGTGACGAGC TTGTTGGCGA ACATTATCTT 301 CATCAACCTG AATCGTGCTT TCCGTGAATA CTTTAAAATT TCCTCTAAGA GCCGTAGCTT 361 TCTGGTTATC TGCGCGTTCA GCAGCCTGCC GATTGTGAAT ATTTGGCTGA TGCTGTGGTG 421 GGTGGCCATG ATTAAAAGAT TTACGAGCAA TTATGCTTTC GCGATTTTTG ATAAGTATAA 481 TGGTCTGACC AGCGGTGTTT TTATCTTCGA CTTTGCAGAT AACGTCAATT TTGAAGGTAA 541 ACTGGTTAGC TTCGATAATA CGAAAGACAC CAATCGCGAC TTCGTTCATT TCTATAGCGA 601 GGCGAAACTG AAACGTGATA AGATCACCCT GCAGACCAAC CCGATCCCGC ACGAGCGTAT 661 GTACGTTAAT CGCATGTATT ACCAACAGAA GCTGAGCATG GGTGCGAACC AGAACTCCCC 721 GAGCACCGCA TTTGCCAACT TGAAAAGATA TGTCGAACAT AAACAGCAAA AGATTATCAA 781 GATTAAACAA TTCATCCTGA CCTGAAGAGC TTAGAGACCC AGCCTCTGTG TTGTCTGATC 841 GCTGAGGTGT CAATCGTCGG AGCCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT 901 AAACGGGTCT TGAGGGGTTT TTTGCATGGT CATAGCTGTT TCCTGAGAGC TTGGCAGGTG 961 ATGACACACA TTAACAAATT TCGTGAGGAG TCTCCAGAAG AATGCCATTA ATTTCCATAG 1021 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 1081 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 1141 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 1201 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 1261 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 1321 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 1381 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 1441 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 1501 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 1561 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGGCCTAC TATTAGCAAC 1621 AACGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA 1681 GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA 1741 TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC 1801 TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA 1861 CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA 1921 ATGATACCGC GAGAACCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC 1981 GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT 2041 TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC 2101 ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT 2161 TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC 2221 TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG 2281 GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT 2341 GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG 2401 GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA 2461 AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG 2521 TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG 2581 TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT 2641 TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 2701 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACC 2761 TGCACCAGTC AGTAAAACGA CGGCCAGTAG TCAAAAGCCT CCGACCGGAG GCTTTTGACT 2821 TGGTCAGGTG GAGTGGGAG //