LOCUS       Exported     2896 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..2896
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      934..981
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     2829..2845
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 for"
     terminator      2846..2877
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..862
                     /translation="MPELPEVATVITELKSCVLNKPVKQVKVHLDKVLKNTNVKQLNDALVNHSFVDIKRRGKYIIFCLSNGLFLVSHLRMEGKYFFEAKGSQFDLNHVLVEFLFQDGDQLNYHDTRQFGTFHLFNRYQFENARELNKLALDPLDQEFNHQAIFNKGHKSNKKIKTFILDQTNISGIGNIYADEILFASKIHPETLAKNLNLSQYQLICQNATDILKKAVEMKGTTIGTFTFKKDHTGGYQHFLKIHGKKGKQCQSCNTTIIKKKINGRGSYICEKCQIQR"
                     /label="fpg"
                     /note="Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized purines, such as 7,8-dihydro-8-oxoguanine (8-oxoG). Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates (By similarity)."
     primer_bind     complement(986..1002)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1069..1657)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(1848..2708)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(2709..2813)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 AAAGCACTCT TAGGCCTCTG GGTCTCAAAT GCCGGAACTG CCTGAGGTTG CCACCGTGAT
       61 CACCGAATTG AAAAGCTGTG TTTTGAACAA ACCAGTCAAA CAAGTGAAGG TGCACTTGGA
      121 TAAGGTCCTC AAAAACACGA ACGTCAAGCA GCTGAACGAT GCGCTGGTGA ATCACAGCTT
      181 CGTTGACATC AAGCGTCGTG GTAAGTACAT CATTTTTTGC TTGTCTAACG GTCTGTTCCT
      241 GGTTAGCCAT CTGCGCATGG AAGGCAAGTA TTTTTTCGAA GCGAAGGGCT CGCAGTTTGA
      301 TCTGAACCAC GTGCTGGTTG AATTTCTGTT TCAGGATGGT GACCAGCTGA ACTATCACGA
      361 CACCCGTCAG TTCGGTACCT TTCACTTATT TAATCGCTAC CAATTCGAGA ATGCCCGCGA
      421 GTTGAACAAA CTGGCATTGG ACCCATTGGA CCAGGAATTC AATCACCAAG CGATTTTCAA
      481 TAAGGGTCAT AAGTCCAATA AAAAGATCAA GACTTTCATT CTGGACCAAA CCAACATTAG
      541 CGGTATCGGT AATATCTATG CGGACGAGAT TCTGTTCGCC AGCAAGATTC ATCCGGAAAC
      601 CCTGGCAAAG AATTTGAACC TGTCGCAATA TCAACTGATC TGTCAGAACG CAACGGACAT
      661 TTTGAAAAAA GCGGTGGAAA TGAAGGGTAC CACCATTGGT ACGTTTACGT TCAAGAAAGA
      721 CCACACCGGT GGTTACCAGC ATTTTCTGAA AATCCATGGT AAAAAGGGTA AGCAATGCCA
      781 GTCCTGCAAC ACGACCATTA TCAAGAAAAA GATTAACGGC CGCGGTAGCT ACATCTGTGA
      841 AAAGTGCCAG ATCCAACGCT GAAGAGCTTA GAGACCGCGG GTTAGATACT GCAACTCGCT
      901 GAGGTGTCAA TCGTCGGAGC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA
      961 CGGGTCTTGA GGGGTTTTTT GCATGGTCAT AGCTGTTTCC TGAGAGCTTG GCAGGTGATG
     1021 ACACACATTA ACAAATTTCG TGAGGAGTCT CCAGAAGAAT GCCATTAATT TCCATAGGCT
     1081 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC
     1141 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC
     1201 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC
     1261 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG
     1321 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA
     1381 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG
     1441 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA
     1501 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG
     1561 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG
     1621 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GGCCTACTAT TAGCAACAAC
     1681 GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG
     1741 ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA
     1801 AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGCTTA
     1861 ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC
     1921 CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG
     1981 ATACCGCGAG AACCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA
     2041 AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT
     2101 TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT
     2161 GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC
     2221 CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC
     2281 GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA
     2341 GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG
     2401 TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG
     2461 TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA
     2521 CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA
     2581 CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA
     2641 GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA
     2701 ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG
     2761 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACCTGC
     2821 ACCAGTCAGT AAAACGACGG CCAGTAGTCA AAAGCCTCCG ACCGGAGGCT TTTGACTTGG
     2881 TCAGGTGGAG TGGGAG
//