LOCUS       Exported     3082 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3082
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1120..1167
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3015..3031
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3032..3063
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..1048
                     /label="lplA"
                     /note="Catalyzes both the ATP-dependent activation of exogenously supplied lipoate to lipoyl-AMP and the transfer of the activated lipoyl onto the lipoyl domains of lipoate-dependent enzymes."
                     /translation="MKTYILTSPKNIPYFNAALEEWLLTEFKKGEEIKVIYFWQNANTIVVGRNQNTYAEVNLSEVEKDKVNLFRRFSGGGAVFHDMGNICFSIILPKAKKEMENAYEETTRNVVKFLNSVGVPAQFHGRNDLEIEGKKFSGLAEYLSKDRVLVHGTLLFDTDFTKLAKYLNVDKTKMVSKGIESVQKRVVNVKEYLPNLSTPTFLEKMVQFFTETEHAETIHLDESSIKMVEKRAQEHFQSWDWNFGKTADYNFKNKKRFEGAGIFECNVQVDQGKVVDIKFYGDFLSVIDITPVTQQLVGQKYDYQTFAKILGNIDNFKEYFGTLTPQQMLEVIFDNKKDE"
     primer_bind     complement(1172..1188)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1255..1843)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2034..2894)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(2895..2999)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 ACAAGGAGTC GGCATATCAC GGTCTCAAAT GAAAACGTAT ATTCTCACCA GCCCGAAGAA
       61 TATCCCTTAT TTTAATGCGG CGCTGGAGGA GTGGTTGCTG ACCGAGTTCA AAAAGGGTGA
      121 GGAGATCAAA GTGATTTATT TTTGGCAAAA CGCGAACACG ATTGTCGTGG GTCGCAATCA
      181 AAACACCTAC GCCGAGGTCA ACCTGAGCGA AGTGGAAAAG GATAAAGTCA ATCTGTTTCG
      241 TCGTTTTAGC GGTGGCGGTG CAGTTTTCCA CGATATGGGC AACATCTGTT TCTCGATCAT
      301 TCTGCCTAAA GCGAAAAAGG AGATGGAAAA CGCGTATGAG GAAACGACTC GTAACGTTGT
      361 GAAGTTTCTG AACTCCGTGG GTGTCCCGGC CCAGTTTCAT GGCCGCAATG ACCTGGAAAT
      421 TGAAGGTAAA AAGTTTAGCG GTCTGGCGGA ATACCTCAGC AAAGACCGCG TCCTGGTTCA
      481 CGGCACGCTG CTGTTCGATA CCGACTTCAC GAAGCTGGCC AAGTATCTGA ACGTGGATAA
      541 GACCAAGATG GTTAGCAAAG GTATTGAGAG CGTTCAGAAA CGTGTCGTTA ATGTCAAAGA
      601 ATATCTGCCT AATCTGAGCA CCCCGACGTT TTTGGAGAAG ATGGTTCAGT TCTTTACCGA
      661 AACCGAACAT GCGGAAACGA TCCACTTGGA TGAGAGCTCC ATTAAGATGG TGGAAAAACG
      721 CGCGCAAGAG CATTTTCAAT CATGGGACTG GAATTTCGGT AAAACCGCGG ATTACAACTT
      781 TAAGAATAAA AAACGTTTCG AAGGTGCTGG CATCTTTGAG TGCAACGTTC AGGTCGATCA
      841 GGGTAAGGTT GTGGACATTA AATTCTATGG TGACTTCTTG TCTGTGATCG ATATCACCCC
      901 AGTGACCCAA CAACTGGTTG GCCAGAAATA CGACTACCAA ACGTTCGCGA AAATCCTGGG
      961 TAATATCGAC AATTTTAAAG AGTACTTCGG TACCCTGACC CCTCAACAGA TGCTGGAAGT
     1021 TATTTTCGAC AATAAGAAAG ATGAGTGAAG AGCTTAGAGA CCGCCATCCT CTTATCTCGT
     1081 GGCGCTGAGG TGTCAATCGT CGGAGCCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC
     1141 TCTAAACGGG TCTTGAGGGG TTTTTTGCAT GGTCATAGCT GTTTCCTGAG AGCTTGGCAG
     1201 GTGATGACAC ACATTAACAA ATTTCGTGAG GAGTCTCCAG AAGAATGCCA TTAATTTCCA
     1261 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA
     1321 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC
     1381 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC
     1441 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT
     1501 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG
     1561 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG
     1621 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA
     1681 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG
     1741 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT
     1801 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGGCC TACTATTAGC
     1861 AACAACGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT
     1921 TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA
     1981 AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA
     2041 TGCTTAATCA GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC
     2101 TGACTCCCCG TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT
     2161 GCAATGATAC CGCGAGAACC ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA
     2221 GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT
     2281 AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT
     2341 GCCATTGCTA CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC
     2401 GGTTCCCAAC GATCAAGGCG AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC
     2461 TCCTTCGGTC CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT
     2521 ATGGCAGCAC TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT
     2581 GGTGAGTACT CAACCAAGTC ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC
     2641 CCGGCGTCAA TACGGGATAA TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT
     2701 GGAAAACGTT CTTCGGGGCG AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG
     2761 ATGTAACCCA CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT
     2821 GGGTGAGCAA AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA
     2881 TGTTGAATAC TCATACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT
     2941 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC
     3001 ACCTGCACCA GTCAGTAAAA CGACGGCCAG TAGTCAAAAG CCTCCGACCG GAGGCTTTTG
     3061 ACTTGGTCAG GTGGAGTGGG AG
//