LOCUS       Exported     3436 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3436
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1474..1521
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
                     /label="T7 terminator"
     primer_bind     3369..3385
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3386..3417
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..1402
                     /label="pdhD"
                     /note="Lipoamide dehydrogenase is a component of the alpha-ketoacid dehydrogenase complexes."
                     /translation="MNYDLIIIGAGPAGYVAAEYAGKHKLKTLVVEKEYFGGVCLNVGCIPTKTLLKRAKIVDYLRHAQDYGISINGQVALNWNQLLEQKGKVVSKLVGGVKAIIASAKAETVMGEAKVLDPNTVEVAGKTYTTKSIVVATGSRPRYLTLPGFAEARQNGFVIDSTQALSLEGVPRKLVVVGGGVIGIEFAFLYASLGSEVTILQGVDRILEIFDTEVSDLVAKLLQTKNVKIITNAQVTRANNNEVFYSQNGQEGSVVGDRILVSIGRIPNTECLDGLNLQRDERNRIVLNQDLQTSIPNIYIVGDANAQLMLAHFAYQQGRYAVNHILNKKQVKPAQKLTCPSCIYTNPEVASVGYTEMELKKQGIPYVKTNLVLAHCGKAIADNETNGFVKMMFDPQTGKILGCCIIAATASDMIAELALAMGAGLTVFDIANSISPHPTINEMIADVCKKALFDHFK"
     primer_bind     complement(1526..1542)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1609..2197)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2388..3248)
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
     promoter        complement(3249..3353)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 AACGATGATG CTCACTCTCG GGTCTCAAAT GAACTACGAC CTGATCATTA TTGGTGCGGG
       61 TCCGGCGGGT TATGTGGCAG CTGAGTATGC GGGCAAGCAC AAGCTGAAAA CGCTGGTTGT
      121 GGAGAAGGAG TACTTTGGTG GCGTCTGCCT GAATGTCGGT TGCATCCCGA CCAAGACGCT
      181 CCTGAAACGC GCGAAAATCG TAGACTATTT GCGTCACGCA CAAGATTACG GTATCAGCAT
      241 TAACGGTCAG GTTGCACTGA ACTGGAATCA ACTGCTGGAG CAGAAGGGCA AGGTTGTCAG
      301 CAAGTTGGTT GGCGGTGTTA AAGCCATTAT CGCCAGCGCC AAGGCTGAAA CCGTGATGGG
      361 TGAAGCCAAG GTTTTGGACC CGAACACCGT CGAAGTGGCG GGTAAGACCT ATACCACCAA
      421 GAGCATCGTT GTGGCCACCG GTTCTCGTCC GCGTTATCTG ACCCTGCCGG GTTTTGCAGA
      481 AGCCCGTCAG AATGGTTTCG TCATTGACTC CACCCAGGCG CTGAGCCTGG AAGGTGTTCC
      541 GCGCAAGCTG GTGGTTGTCG GCGGCGGTGT GATTGGCATT GAGTTCGCGT TTCTGTACGC
      601 CAGCCTGGGT TCAGAAGTGA CCATTCTGCA AGGTGTGGAC CGTATTCTGG AGATTTTTGA
      661 CACCGAGGTT AGCGATCTTG TCGCAAAACT GCTGCAGACC AAGAACGTGA AAATTATCAC
      721 GAATGCGCAA GTCACCCGTG CGAACAACAA TGAGGTTTTC TATAGCCAGA ACGGCCAAGA
      781 AGGTAGCGTG GTTGGCGACC GTATTCTGGT TTCCATCGGT CGTATTCCGA ATACCGAGTG
      841 TCTGGACGGT TTGAATCTGC AGCGTGATGA GCGCAATCGC ATTGTGCTGA ATCAGGATCT
      901 GCAGACCTCC ATCCCGAACA TCTACATCGT GGGTGACGCT AACGCGCAAC TGATGCTTGC
      961 TCACTTCGCG TATCAGCAGG GTCGTTATGC GGTGAATCAC ATTCTGAACA AAAAGCAAGT
     1021 CAAGCCGGCC CAGAAGCTGA CGTGTCCGAG CTGCATTTAC ACCAACCCGG AGGTCGCATC
     1081 CGTCGGCTAC ACTGAGATGG AGCTGAAAAA ACAAGGTATT CCGTACGTTA AGACCAACCT
     1141 GGTGCTGGCT CACTGTGGTA AGGCGATTGC AGATAACGAA ACGAATGGTT TCGTCAAGAT
     1201 GATGTTTGAT CCGCAGACCG GTAAAATTTT GGGTTGCTGT ATCATTGCGG CGACCGCGAG
     1261 CGACATGATC GCGGAGCTGG CGCTGGCTAT GGGCGCCGGT CTGACTGTCT TTGATATCGC
     1321 AAACTCCATT TCCCCGCATC CGACCATCAA CGAGATGATT GCCGACGTTT GTAAGAAAGC
     1381 CTTGTTTGAC CATTTTAAGT GAAGAGCTTA GAGACCGAGT CGTCCAACCC TTGAATCGCT
     1441 GAGGTGTCAA TCGTCGGAGC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA
     1501 CGGGTCTTGA GGGGTTTTTT GCATGGTCAT AGCTGTTTCC TGAGAGCTTG GCAGGTGATG
     1561 ACACACATTA ACAAATTTCG TGAGGAGTCT CCAGAAGAAT GCCATTAATT TCCATAGGCT
     1621 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC
     1681 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC
     1741 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC
     1801 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG
     1861 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA
     1921 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG
     1981 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA
     2041 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG
     2101 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG
     2161 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GGCCTACTAT TAGCAACAAC
     2221 GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG
     2281 ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA
     2341 AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGCTTA
     2401 ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC
     2461 CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG
     2521 ATACCGCGAG AACCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA
     2581 AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT
     2641 TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT
     2701 GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC
     2761 CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC
     2821 GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA
     2881 GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG
     2941 TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG
     3001 TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA
     3061 CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA
     3121 CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA
     3181 GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA
     3241 ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG
     3301 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACCTGC
     3361 ACCAGTCAGT AAAACGACGG CCAGTAGTCA AAAGCCTCCG ACCGGAGGCT TTTGACTTGG
     3421 TCAGGTGGAG TGGGAG
//