LOCUS Exported 3046 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..3046 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 1084..1131 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 2979..2995 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 2996..3027 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 29..1012 /label="pdhB" /note="The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3) (By similarity)." /translation="MSKTIQANNIEALGNAMDLALERDPNVVLYGQDAGFEGGVFRATKGLQKKYGEERVWDCPIAEAAMAGIGVGAAIGGLKPIVEIQFSGFSFPAMFQIFTHAARIRNRSRGVYTCPIIVRMPMGGGIKALEHHSETLEAIYGQIAGLKTVMPSNPYDTKGLFLAAVESPDPVVFFEPKKLYRAFRQEIPADYYTVPIGQANLISQGNNLTIVSYGPTMFDLINMVYGGELKDKGIELIDLRTISPWDKETVFNSVKKTGRLLVVTEAAKTFTTSGEIIASVTEELFSYLKAAPQRVTGWDIVVPLARGEHYQFNLNARILEAVNQLLK" primer_bind complement(1136..1152) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(1219..1807) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(1998..2858) /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" promoter complement(2859..2963) /label="bla promoter" /note="ampicillin resistance gene promoter" ORIGIN 1 AAACCGGAGC CATACAGTAC GGTCTCAAAT GAGCAAGACT ATTCAGGCGA ACAATATCGA 61 GGCCCTGGGC AATGCAATGG ACCTGGCGTT GGAACGCGAT CCGAACGTTG TTCTGTATGG 121 TCAAGATGCG GGTTTCGAAG GCGGTGTGTT TCGCGCGACC AAAGGTCTGC AGAAAAAGTA 181 TGGTGAGGAG CGTGTTTGGG ATTGCCCGAT CGCGGAAGCG GCTATGGCGG GCATTGGTGT 241 GGGTGCAGCG ATCGGCGGCC TGAAGCCGAT TGTTGAGATT CAATTTTCCG GCTTCAGCTT 301 TCCGGCAATG TTCCAGATCT TTACGCATGC GGCTCGTATC CGCAATCGCA GCCGTGGCGT 361 GTATACTTGC CCGATCATCG TCCGCATGCC GATGGGTGGT GGTATCAAGG CGCTGGAGCA 421 CCATAGCGAA ACCCTGGAAG CAATCTACGG TCAGATTGCG GGCCTCAAGA CCGTTATGCC 481 GAGCAATCCG TACGACACCA AAGGTCTGTT CCTGGCGGCG GTGGAGAGCC CGGACCCAGT 541 TGTCTTTTTT GAGCCGAAGA AGCTGTACCG TGCCTTCCGT CAGGAGATCC CAGCGGACTA 601 CTACACGGTG CCGATTGGCC AAGCCAATCT GATCAGCCAG GGTAACAATC TGACCATCGT 661 TAGCTACGGT CCGACCATGT TCGATCTGAT CAACATGGTC TATGGTGGTG AGCTGAAGGA 721 CAAGGGTATC GAATTGATCG ATCTGCGCAC CATTAGCCCT TGGGACAAGG AAACGGTTTT 781 CAATTCAGTC AAGAAAACCG GTCGCCTGCT GGTTGTTACG GAGGCGGCCA AAACGTTTAC 841 CACGTCGGGC GAGATCATTG CCAGCGTCAC TGAGGAGCTG TTTTCTTACT TGAAAGCGGC 901 TCCGCAGCGT GTTACTGGTT GGGACATCGT GGTGCCGCTG GCGCGTGGCG AACATTACCA 961 GTTTAATTTG AATGCACGTA TCTTGGAGGC GGTGAATCAA CTGCTGAAGT GAAGAGCTTA 1021 GAGACCGCCA TCCTCTTATC TCGTGGCGCT GAGGTGTCAA TCGTCGGAGC CGCTGAGCAA 1081 TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCATGGTCAT 1141 AGCTGTTTCC TGAGAGCTTG GCAGGTGATG ACACACATTA ACAAATTTCG TGAGGAGTCT 1201 CCAGAAGAAT GCCATTAATT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 1261 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 1321 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 1381 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 1441 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 1501 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 1561 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 1621 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 1681 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 1741 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 1801 ATCTCAAGAA GGCCTACTAT TAGCAACAAC GATCCTTTGA TCTTTTCTAC GGGGTCTGAC 1861 GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC 1921 TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG 1981 TAAACTTGGT CTGACAGTTA CCAATGCTTA ATCAGTGAGG CACCTATCTC AGCGATCTGT 2041 CTATTTCGTT CATCCATAGT TGCCTGACTC CCCGTCGTGT AGATAACTAC GATACGGGAG 2101 GGCTTACCAT CTGGCCCCAG TGCTGCAATG ATACCGCGAG AACCACGCTC ACCGGCTCCA 2161 GATTTATCAG CAATAAACCA GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG TCCTGCAACT 2221 TTATCCGCCT CCATCCAGTC TATTAATTGT TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA 2281 GTTAATAGTT TGCGCAACGT TGTTGCCATT GCTACAGGCA TCGTGGTGTC ACGCTCGTCG 2341 TTTGGTATGG CTTCATTCAG CTCCGGTTCC CAACGATCAA GGCGAGTTAC ATGATCCCCC 2401 ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG 2461 GCCGCAGTGT TATCACTCAT GGTTATGGCA GCACTGCATA ATTCTCTTAC TGTCATGCCA 2521 TCCGTAAGAT GCTTTTCTGT GACTGGTGAG TACTCAACCA AGTCATTCTG AGAATAGTGT 2581 ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG TCAATACGGG ATAATACCGC GCCACATAGC 2641 AGAACTTTAA AAGTGCTCAT CATTGGAAAA CGTTCTTCGG GGCGAAAACT CTCAAGGATC 2701 TTACCGCTGT TGAGATCCAG TTCGATGTAA CCCACTCGTG CACCCAACTG ATCTTCAGCA 2761 TCTTTTACTT TCACCAGCGT TTCTGGGTGA GCAAAAACAG GAAGGCAAAA TGCCGCAAAA 2821 AAGGGAATAA GGGCGACACG GAAATGTTGA ATACTCATAC TCTTCCTTTT TCAATATTAT 2881 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 2941 AATAAACAAA TAGGGGTTCC GCGCACCTGC ACCAGTCAGT AAAACGACGG CCAGTAGTCA 3001 AAAGCCTCCG ACCGGAGGCT TTTGACTTGG TCAGGTGGAG TGGGAG //