LOCUS       Exported     3514 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3514
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
                     /label="T7 terminator"
     terminator      1552..1599
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3447..3463
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3464..3495
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..1480
                     /label="proS"
                     /note="Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro)."
                     /translation="MANKDQNLTLWYDQLLSKAQLVSYGDVKGTNCFLPNSWNLWLQIQRLYNNATALIKLKDKVILKQFIPIEPLPYTVEQVQLPTLSFYSEYQKEKRHVEGFNPELFLIEQIGTKKLHDPLVLRPTSEIAFCNLWKKQSFSYQNLPVIYNQWTCVFRAEKNTRPFLRNSEFYWQETHGLFSDGVNSESAAIAFWKLYQDIIVNQLCIPAFVGLKSPNERFAGAQNTWTVESIMPDGQALQCATSHDLGQTFTKPFGLTFQNQANQQAIPYSFSCGISTRILGALLLTHSDDFGLVLPWKVAPIQVKLYLFDKKGDTKTVELAQKVQTLLEQLAIRFQFIKVENQLGKQLGQGEVNGIPFQLIVDNPQTVNIFNRLTRVKTAYSFEQLASRFVELVQQYHQAMYDKAKAVVQQKVVQATTLKQIEQAFNDKKAVLCAVRLTDTLEQQLKERYQVTVRCCLEQLQKPQICPFSGESAQDYVLIARAY"
     primer_bind     complement(1604..1620)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1687..2275)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2466..3326)
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
     promoter        complement(3327..3431)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 AAATAAGACG ACGACCCTCG GGTCTCAAAT GGCGAACAAA GATCAGAACC TGACTTTGTG
       61 GTACGACCAG CTGCTGAGCA AAGCGCAACT GGTTAGCTAT GGTGACGTGA AAGGTACCAA
      121 TTGCTTTCTG CCGAATAGCT GGAACCTGTG GCTGCAGATC CAACGTCTGT ACAACAACGC
      181 CACCGCGCTG ATCAAGCTGA AGGACAAAGT TATTCTGAAA CAGTTTATCC CGATCGAGCC
      241 GCTGCCGTAC ACGGTCGAAC AGGTGCAACT GCCGACCCTG AGCTTTTACT CTGAGTACCA
      301 GAAAGAAAAG CGTCATGTGG AAGGTTTTAA TCCGGAGCTG TTCCTGATCG AGCAAATTGG
      361 TACCAAAAAA CTGCATGACC CGCTGGTTTT GCGTCCGACG TCGGAAATCG CGTTCTGTAA
      421 TCTGTGGAAA AAACAGAGCT TTAGCTACCA AAATCTGCCG GTGATCTACA ATCAGTGGAC
      481 GTGCGTTTTT CGTGCGGAGA AAAACACCCG CCCGTTTTTA CGTAACAGCG AGTTCTACTG
      541 GCAAGAAACC CATGGTCTGT TCTCCGACGG TGTGAACAGC GAGTCTGCGG CGATTGCCTT
      601 CTGGAAGCTG TACCAAGACA TTATCGTGAA CCAGCTGTGC ATTCCGGCGT TTGTCGGCCT
      661 TAAGAGCCCG AACGAACGCT TCGCAGGCGC TCAAAACACC TGGACCGTCG AGAGCATTAT
      721 GCCGGATGGT CAGGCATTAC AATGTGCAAC GTCCCATGAC CTGGGTCAAA CCTTCACCAA
      781 GCCGTTTGGT TTAACGTTCC AAAACCAAGC AAATCAACAG GCAATCCCGT ACAGCTTCAG
      841 CTGCGGTATT AGCACCCGTA TTCTGGGCGC GCTGCTGCTG ACCCACTCCG ATGATTTCGG
      901 TCTGGTTCTG CCGTGGAAGG TGGCACCAAT TCAGGTTAAA CTGTATCTGT TTGACAAGAA
      961 AGGTGATACG AAAACTGTTG AGCTGGCCCA GAAAGTCCAG ACCCTGCTGG AACAACTGGC
     1021 CATTCGCTTT CAGTTCATTA AAGTGGAGAA CCAGCTGGGC AAGCAGCTTG GTCAAGGTGA
     1081 GGTCAACGGC ATCCCGTTTC AATTGATCGT CGACAACCCG CAAACGGTTA ATATCTTTAA
     1141 TCGCCTGACG CGTGTAAAGA CCGCATATTC TTTTGAACAG TTGGCGAGCC GTTTTGTGGA
     1201 GCTGGTGCAG CAATACCACC AGGCAATGTA CGACAAGGCT AAGGCGGTGG TTCAACAAAA
     1261 AGTTGTCCAG GCCACCACCC TGAAGCAAAT TGAACAAGCA TTTAATGATA AAAAGGCCGT
     1321 GCTGTGCGCG GTCCGTCTGA CGGACACCCT GGAGCAACAA CTGAAAGAGC GTTACCAGGT
     1381 TACCGTGCGC TGTTGTCTGG AGCAACTGCA AAAGCCGCAG ATCTGCCCGT TCTCCGGCGA
     1441 GTCGGCGCAG GATTACGTGC TGATCGCGCG CGCCTACTGA AGAGCTTAGA GACCGTTACA
     1501 CCGGGATCAT AGCTCGCTGA GGTGTCAATC GTCGGAGCCG CTGAGCAATA ACTAGCATAA
     1561 CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC ATGGTCATAG CTGTTTCCTG
     1621 AGAGCTTGGC AGGTGATGAC ACACATTAAC AAATTTCGTG AGGAGTCTCC AGAAGAATGC
     1681 CATTAATTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA
     1741 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT
     1801 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC
     1861 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT
     1921 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC
     1981 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC
     2041 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG
     2101 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC
     2161 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG
     2221 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGG
     2281 CCTACTATTA GCAACAACGA TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC
     2341 GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC
     2401 CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT
     2461 GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA
     2521 TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT
     2581 GGCCCCAGTG CTGCAATGAT ACCGCGAGAA CCACGCTCAC CGGCTCCAGA TTTATCAGCA
     2641 ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC
     2701 ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG
     2761 CGCAACGTTG TTGCCATTGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT
     2821 TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA
     2881 AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA
     2941 TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC
     3001 TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG
     3061 AGTTGCTCTT GCCCGGCGTC AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA
     3121 GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG
     3181 AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC
     3241 ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG
     3301 GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT
     3361 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA
     3421 GGGGTTCCGC GCACCTGCAC CAGTCAGTAA AACGACGGCC AGTAGTCAAA AGCCTCCGAC
     3481 CGGAGGCTTT TGACTTGGTC AGGTGGAGTG GGAG
//