LOCUS       Exported     4345 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..4345
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      2383..2430
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     4278..4294
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      4295..4326
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..2311
                     /label="MPN408"
                     /note=""
                     /translation="MVIKKGFFALSSCTLGLGLILTACGARGKFDQVDDGVIKLATSIQNKDSIAALNTIYKKYKERHPGSYPVQNFQVPGGYSGLRNDIRTRLSAKDGNNFYNIVLNYPDVVSSLATSDMQLILDNVDTKLLSKNFLSFNERIGGVRQKGIYAIPISLSTELMVLNGPVLHYILNSAKKKENGAQMIKRSGSFSTVQKGTMAIDMNDQKTKDLWQKIENAAKANGKTTSTQTSPQPKNAVSSLQLKQAAEGTSTDNSQDAENSDNEIKKTWGEYKEEGNHTLKGYTFKASVFENWNELLDFSTRVANSFPDKIKNQASKKATELQGVFGVDSVSGALFSATFAAGGGDYDKFFFNVKNGRGNFRNLLEKGSSYNNLQKVFNDYKQLISSNGLYINKGGAYSSNFLKFHQLAFSVGSSSGYHFAFAGESAKRLEFGQKAIEYPRDTYEIKAPTNSQNGNGTLLGSFTKSKSNGKEQSGQDEDNQTSETIELYKSSVPSGKEAGKNALAITNQQLISALENAAKDNKTSQPQARSLTASDQVQITQSSDKVIGYITTSNLDIDNNNTFDVGKLNGDKSTSKIIVNATLKTLNKINTLQSEEGIILPHPQKYKSTDPQAVATVQGPSIIGVHANAKENAETQKFINWFINQKETWPENSKGNKNGQNGQMTAAQYFAKSSGYVLPYSETFTKQSEDEHSTTKDAYKILKDVNDGKLVGYSDPSDFRSGKFRDTIVAAFSGAVSSKADFNKFFKGFEQQLGQEYRRG"
     primer_bind     complement(2435..2451)
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 rev"
     rep_origin      complement(2518..3106)
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
                     /label="ori"
     CDS             complement(3297..4157)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(4158..4262)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 AAATAAGACG ACGACCCTCG GGTCTCAAAT GGTGATCAAG AAAGGCTTTT TTGCCCTGTC
       61 CAGCTGTACC TTAGGTCTGG GTTTGATTCT GACCGCATGC GGCGCGCGCG GTAAATTCGA
      121 CCAAGTCGAC GATGGTGTCA TTAAGCTGGC TACGAGCATT CAAAATAAAG ACTCTATCGC
      181 GGCACTGAAC ACCATCTATA AAAAATATAA AGAGCGCCAT CCGGGTAGCT ACCCGGTCCA
      241 AAATTTCCAG GTCCCAGGCG GTTATAGCGG CCTGCGTAAC GATATCCGTA CGCGTCTGAG
      301 CGCGAAGGAC GGTAACAACT TCTATAACAT TGTTCTGAAC TATCCGGATG TCGTGTCGAG
      361 CCTGGCCACG AGCGATATGC AGCTGATTCT GGACAATGTG GATACGAAGC TGCTGTCAAA
      421 AAATTTTTTG AGCTTTAACG AGCGTATCGG TGGCGTCCGC CAAAAAGGCA TTTACGCCAT
      481 TCCAATTAGC CTGAGCACCG AACTGATGGT TCTGAATGGT CCGGTGCTGC ACTACATTTT
      541 AAATAGCGCG AAAAAGAAGG AGAATGGTGC GCAAATGATT AAACGTAGCG GTAGCTTCAG
      601 CACGGTTCAA AAAGGTACGA TGGCAATTGA TATGAATGAT CAAAAGACGA AAGATTTGTG
      661 GCAAAAGATT GAGAATGCGG CGAAGGCCAA CGGCAAGACC ACGAGCACCC AAACCAGCCC
      721 GCAACCGAAG AATGCCGTGT CTAGCCTGCA GCTGAAGCAA GCGGCCGAGG GCACCAGCAC
      781 CGACAATTCC CAAGACGCGG AAAACAGCGA TAACGAGATT AAGAAAACCT GGGGCGAGTA
      841 CAAGGAAGAG GGCAACCATA CGCTGAAGGG TTACACCTTT AAGGCAAGCG TCTTTGAGAA
      901 TTGGAACGAG CTGCTGGACT TCAGCACCCG TGTCGCGAAC AGCTTTCCTG ATAAGATTAA
      961 AAACCAGGCG AGCAAGAAAG CCACTGAACT GCAGGGCGTC TTTGGTGTCG ACAGCGTGAG
     1021 CGGCGCGCTG TTCAGCGCGA CCTTCGCCGC GGGCGGTGGC GATTACGATA AATTCTTTTT
     1081 TAACGTTAAA AACGGTCGTG GTAATTTCCG CAACTTGCTG GAAAAAGGTT CCAGCTATAA
     1141 TAATCTGCAG AAGGTTTTCA ATGACTATAA ACAACTGATC AGCAGCAATG GCCTGTATAT
     1201 TAACAAGGGT GGCGCGTATA GCAGCAACTT TCTGAAATTC CATCAACTGG CCTTTTCCGT
     1261 CGGCTCTAGC AGCGGCTACC ATTTCGCATT TGCGGGCGAG AGCGCGAAGC GCCTGGAGTT
     1321 CGGTCAGAAA GCGATCGAGT ACCCGCGTGA TACCTACGAA ATTAAAGCTC CGACCAATAG
     1381 CCAGAATGGT AACGGTACCC TGTTGGGCAG CTTTACCAAA TCAAAGAGCA ATGGCAAAGA
     1441 ACAGAGCGGC CAAGATGAAG ATAATCAGAC TAGCGAAACC ATCGAGCTGT ACAAAAGCAG
     1501 CGTGCCGAGC GGTAAGGAGG CTGGTAAAAA CGCCCTGGCC ATCACGAACC AACAGCTGAT
     1561 CAGCGCGCTT GAGAACGCCG CGAAAGACAA TAAGACGAGC CAGCCGCAAG CGCGTAGCCT
     1621 GACGGCTTCC GATCAAGTGC AAATTACCCA AAGCAGCGAC AAGGTTATCG GTTACATTAC
     1681 GACGAGCAAT TTGGACATTG ATAATAATAA TACTTTCGAC GTGGGCAAAC TGAACGGTGA
     1741 TAAATCAACG TCCAAGATCA TCGTGAATGC GACGCTGAAA ACCCTGAATA AGATTAATAC
     1801 CTTGCAGAGC GAGGAAGGTA TTATTCTGCC GCACCCGCAG AAGTATAAGT CTACGGACCC
     1861 GCAAGCTGTG GCCACGGTGC AAGGCCCTAG CATCATTGGT GTTCACGCCA ACGCCAAGGA
     1921 GAACGCCGAA ACCCAAAAGT TCATTAACTG GTTTATTAAT CAGAAAGAAA CCTGGCCGGA
     1981 AAATAGCAAG GGCAACAAAA ATGGTCAAAA CGGTCAAATG ACCGCTGCTC AATACTTCGC
     2041 CAAGAGCTCT GGCTATGTTC TGCCGTACAG CGAAACCTTC ACCAAACAGA GCGAGGATGA
     2101 ACACTCGACG ACCAAGGACG CCTATAAGAT TCTGAAAGAC GTCAACGATG GTAAATTGGT
     2161 CGGTTACAGC GATCCGAGCG ACTTCCGTAG CGGTAAATTC CGCGACACCA TCGTGGCGGC
     2221 TTTCTCCGGT GCTGTTAGCA GCAAAGCGGA TTTTAACAAA TTCTTTAAAG GCTTTGAGCA
     2281 ACAGCTGGGT CAGGAATATC GTCGTGGCTG AAGAGCTTAG AGACCGCCAT CCTCTTATCT
     2341 CGTGGCGCTG AGGTGTCAAT CGTCGGAGCC GCTGAGCAAT AACTAGCATA ACCCCTTGGG
     2401 GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CATGGTCATA GCTGTTTCCT GAGAGCTTGG
     2461 CAGGTGATGA CACACATTAA CAAATTTCGT GAGGAGTCTC CAGAAGAATG CCATTAATTT
     2521 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG
     2581 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC
     2641 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT
     2701 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA
     2761 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA
     2821 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA
     2881 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA
     2941 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT
     3001 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT
     3061 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG GCCTACTATT
     3121 AGCAACAACG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA
     3181 CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT
     3241 TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC
     3301 CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT
     3361 GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT
     3421 GCTGCAATGA TACCGCGAGA ACCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG
     3481 CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT
     3541 ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT
     3601 GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC
     3661 TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA AAAAGCGGTT
     3721 AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG
     3781 GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG
     3841 ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT
     3901 TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC
     3961 ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT GAGATCCAGT
     4021 TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT
     4081 TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG
     4141 AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT
     4201 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG
     4261 CGCACCTGCA CCAGTCAGTA AAACGACGGC CAGTAGTCAA AAGCCTCCGA CCGGAGGCTT
     4321 TTGACTTGGT CAGGTGGAGT GGGAG
//