LOCUS       Exported     3544 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3544
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1582..1629
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3477..3493
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3494..3525
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..1510
                     /label="MPN414"
                     /note=""
                     /translation="MKPTSLPKNFTNNPAPKAVTGFKLDNGRAYRKLNEAWPVYEPLDSTKDGKGKDKDGWTTSGASEPKGDAPLVSSTGSQMSAVTDSQQSGHNSGLVSLAQRSTTVAVQKSDSSGFQGQGTTDNKFQKYLNTAQALHQMGVIVPSLETWPGKPSTGIATRAGGGVSVQAATRQSSSTNEDLPNVITQLYHTSTSQLAYLNGQIVVMGSNAVPSLWYWVVDERTTSGSATWWAKTHLNFGTEVQKSFVENQLGFKDDSNSDSKNSNLKAQDLTQPAYLITGLDVVQDHLVFAAFKAGAVGYDMTTDSNASTKDQALAWSTTAGLDSAGGYNNLVENTAGLNGPINDLFTLLDTFAYVTPVSGMKGGSQNNEEVQTKYPVKDDSKASAKIASLINASPLNSYGDDGVTVFDALGLNFNFKLDEARLPSRTDQLLVYGIVNESELKSARENAQSTSDDNSNTKVKWTKPPRTTSPCRITTVPISPKRVTEEERSSGNH"
     primer_bind     complement(1634..1650)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1717..2305)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2496..3356)
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
     promoter        complement(3357..3461)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 AATGCGTCAT TTTACACGGC GGTCTCAAAT GAAGCCGACC AGCCTGCCGA AGAATTTTAC
       61 CAATAATCCG GCGCCGAAAG CTGTCACCGG TTTTAAATTA GACAACGGTC GCGCTTACCG
      121 TAAGCTGAAT GAGGCGTGGC CGGTGTACGA GCCTCTGGAC AGCACCAAAG ACGGTAAAGG
      181 TAAAGACAAG GACGGTTGGA CCACCAGCGG TGCGAGCGAA CCGAAGGGCG ACGCGCCGTT
      241 GGTCAGCAGC ACCGGTAGCC AGATGAGCGC AGTCACCGAT TCTCAGCAGA GCGGTCATAA
      301 CAGCGGCTTG GTGAGCCTGG CGCAACGTAG CACCACCGTC GCCGTCCAGA AAAGCGACTC
      361 CAGCGGTTTT CAGGGTCAAG GCACCACGGA CAATAAGTTC CAAAAGTACC TGAATACTGC
      421 ACAAGCACTG CACCAAATGG GTGTTATTGT CCCTAGCCTC GAAACCTGGC CGGGTAAACC
      481 GAGCACGGGC ATTGCGACCC GCGCGGGTGG TGGCGTGAGC GTTCAAGCTG CCACGCGCCA
      541 ATCCAGCAGC ACCAATGAGG ATCTGCCGAA TGTTATTACC CAATTGTACC ACACGTCGAC
      601 CTCACAGCTG GCCTATCTGA ATGGTCAGAT CGTTGTCATG GGTAGCAACG CAGTTCCGAG
      661 CCTGTGGTAC TGGGTGGTGG ACGAACGTAC TACCTCAGGT AGCGCCACCT GGTGGGCCAA
      721 GACCCACCTG AACTTCGGTA CGGAAGTCCA GAAAAGCTTC GTTGAGAACC AGCTGGGTTT
      781 CAAGGATGAC TCTAATAGCG ACTCGAAGAA TTCCAATCTG AAGGCACAGG ACCTGACCCA
      841 GCCGGCGTAC TTGATCACCG GTCTGGACGT AGTTCAAGAC CACCTGGTGT TTGCCGCGTT
      901 CAAGGCTGGT GCAGTTGGTT ATGACATGAC CACCGACAGC AATGCAAGCA CCAAAGATCA
      961 GGCGCTGGCT TGGTCTACCA CGGCGGGTCT GGACAGCGCG GGTGGTTATA ATAATCTGGT
     1021 TGAAAACACC GCAGGCCTGA ACGGCCCGAT TAACGACCTG TTCACCCTGC TGGACACCTT
     1081 TGCATACGTC ACGCCGGTTA GCGGCATGAA GGGTGGTAGC CAGAACAACG AGGAGGTTCA
     1141 GACCAAGTAT CCGGTGAAGG ACGACTCCAA AGCAAGCGCA AAAATTGCGA GCTTGATCAA
     1201 TGCAAGCCCG CTGAATTCCT ATGGTGATGA CGGTGTTACT GTGTTTGATG CACTGGGTCT
     1261 GAACTTTAAC TTCAAACTGG ACGAAGCGCG CCTGCCTAGC CGTACGGACC AACTGCTGGT
     1321 GTACGGTATC GTTAACGAGA GCGAGCTCAA GAGCGCACGT GAGAACGCAC AGAGCACGAG
     1381 CGACGACAAT AGCAACACCA AAGTGAAGTG GACGAAACCG CCTCGTACCA CTAGCCCGTG
     1441 CCGCATCACC ACCGTCCCTA TTAGCCCGAA GCGTGTAACC GAGGAGGAGC GTTCTTCTGG
     1501 CAACCATTGA AGAGCTTAGA GACCGGCTCG ATACAGTTTC ACGTCGCTGA GGTGTCAATC
     1561 GTCGGAGCCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG
     1621 GGTTTTTTGC ATGGTCATAG CTGTTTCCTG AGAGCTTGGC AGGTGATGAC ACACATTAAC
     1681 AAATTTCGTG AGGAGTCTCC AGAAGAATGC CATTAATTTC CATAGGCTCC GCCCCCCTGA
     1741 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG
     1801 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT
     1861 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG
     1921 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC
     1981 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT
     2041 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA
     2101 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC
     2161 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC
     2221 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT
     2281 TACGCGCAGA AAAAAAGGAT CTCAAGAAGG CCTACTATTA GCAACAACGA TCCTTTGATC
     2341 TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG
     2401 AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA
     2461 ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA
     2521 CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG
     2581 ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAA
     2641 CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC
     2701 AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT
     2761 AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC TACAGGCATC
     2821 GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT CCGGTTCCCA ACGATCAAGG
     2881 CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG TCCTCCGATC
     2941 GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG TTATGGCAGC ACTGCATAAT
     3001 TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA CTGGTGAGTA CTCAACCAAG
     3061 TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT GCCCGGCGTC AATACGGGAT
     3121 AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA TTGGAAAACG TTCTTCGGGG
     3181 CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT CGATGTAACC CACTCGTGCA
     3241 CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA
     3301 AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA AATGTTGAAT ACTCATACTC
     3361 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA
     3421 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACCTGCAC CAGTCAGTAA
     3481 AACGACGGCC AGTAGTCAAA AGCCTCCGAC CGGAGGCTTT TGACTTGGTC AGGTGGAGTG
     3541 GGAG
//