LOCUS       Exported     2935 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..2935
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      973..1020
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     2868..2884
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      2885..2916
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..901
                     /label="yidA"
                     /note=""
                     /translation="MTKTSKASGLSWFFCDLDGTLLRYQNNQHLIEPTTKRAVAQLVESGANFVVATGRKPSDVRNIYKELGIEQASPYLIANNGAVVWDLKRNSYLNKQTLSLSDFDLIDHINQTLNQLNHEYGCILYGLNDQVYFYHIHAPDSQAFKQYFAFYEGEFVQNQYLEIDGLKTEYNLVKAIWFFKEVHQQKAVIAQHFTNQERLVITSAHSFELVPLNVSKGHAINLIKQQVKITDNQIMVLGDSYNDLPMFQHGVVKVTNHLAPDNLKQLATRVYELPASLFVGQALNDYFKFD"
     primer_bind     complement(1025..1041)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1108..1696)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(1887..2747)
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
     promoter        complement(2748..2852)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 AACGATGATG CTCACTCTCG GGTCTCAAAT GACCAAAACC TCCAAGGCTT CCGGTCTGTC
       61 GTGGTTCTTC TGTGACCTGG ACGGTACGCT GCTGCGCTAC CAAAATAATC AGCATCTGAT
      121 CGAACCTACG ACCAAGCGTG CGGTTGCACA ACTGGTGGAA TCGGGTGCGA ATTTCGTTGT
      181 CGCGACCGGT CGTAAGCCAA GCGACGTGCG TAACATTTAT AAGGAGTTGG GTATCGAGCA
      241 AGCGAGCCCG TACCTGATCG CGAACAATGG CGCAGTCGTT TGGGACCTCA AACGCAACAG
      301 CTACTTGAAT AAGCAAACTT TAAGCTTGAG CGACTTCGAT CTGATTGACC ACATCAATCA
      361 GACCCTGAAC CAACTGAACC ACGAGTACGG CTGCATTCTG TACGGCCTGA ACGACCAAGT
      421 CTATTTCTAT CACATTCACG CTCCGGATTC CCAGGCTTTC AAGCAATACT TTGCGTTCTA
      481 TGAAGGCGAG TTCGTCCAAA ATCAATATCT GGAGATCGAC GGCCTCAAGA CCGAATATAA
      541 CCTGGTGAAG GCTATTTGGT TTTTTAAAGA GGTTCACCAG CAGAAAGCGG TTATCGCGCA
      601 ACATTTTACG AATCAGGAGC GTCTGGTCAT TACGTCCGCG CATAGCTTCG AGCTGGTCCC
      661 GTTGAACGTG AGCAAGGGCC ACGCGATCAA CCTGATTAAA CAACAAGTGA AGATTACCGA
      721 CAACCAAATT ATGGTCTTGG GTGATAGCTA CAATGATCTG CCGATGTTCC AACACGGTGT
      781 GGTCAAAGTC ACGAATCACC TGGCGCCGGA CAATCTGAAA CAGCTGGCGA CCCGTGTTTA
      841 TGAACTGCCG GCGTCCCTGT TCGTGGGCCA GGCTTTGAAC GACTACTTCA AATTCGATTG
      901 AAGAGCTTAG AGACCCCGGT TGTACCTATC GAGTGCGCTG AGGTGTCAAT CGTCGGAGCC
      961 GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG
     1021 CATGGTCATA GCTGTTTCCT GAGAGCTTGG CAGGTGATGA CACACATTAA CAAATTTCGT
     1081 GAGGAGTCTC CAGAAGAATG CCATTAATTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA
     1141 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC
     1201 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA
     1261 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA
     1321 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA
     1381 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA
     1441 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG
     1501 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG
     1561 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG
     1621 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG
     1681 AAAAAAAGGA TCTCAAGAAG GCCTACTATT AGCAACAACG ATCCTTTGAT CTTTTCTACG
     1741 GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA
     1801 AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT
     1861 ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA
     1921 GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG
     1981 ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA ACCACGCTCA
     2041 CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT
     2101 CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT
     2161 AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA
     2221 CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA
     2281 TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA
     2341 AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT
     2401 GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA
     2461 GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG
     2521 CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC
     2581 TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA
     2641 TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT
     2701 GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT
     2761 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT
     2821 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACCTGCA CCAGTCAGTA AAACGACGGC
     2881 CAGTAGTCAA AAGCCTCCGA CCGGAGGCTT TTGACTTGGT CAGGTGGAGT GGGAG
//