LOCUS       Exported     2875 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..2875
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      913..960
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     2808..2824
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      2825..2856
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..841
                     /label="cbiO"
                     /note=""
                     /translation="MLNVTNLSFTYPRHSQPALKKLNFSLKPNTHVAIIGHNGSGKSTLVKLLGGFLKAPKQTIFFRGQPLEEVGFRQIGILLQDPDMQLLGDTLHQELIFSLENHGVDPKQMDRVIADVLAVVELQGKQFTPLSKLSFGEKQRAVFACLLAVKPELYLLDEAFSMLDGKLASKLKRFIFKVIKEQQKTVINVTHDFNDLFLADEIIFLSKGQLLKQFSPAAIYKQLHLFHQHHFTLPFPWLLAHEVADHLHHEMKGPIEELQDVVDWICKHLK"
     primer_bind     complement(965..981)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1048..1636)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(1827..2687)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(2688..2792)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 AAATAAGACG ACGACCCTCG GGTCTCAAAT GCTGAATGTG ACGAACCTGA GCTTTACCTA
       61 TCCGCGCCAC TCGCAACCGG CGCTGAAAAA ACTGAATTTC AGCCTGAAAC CGAACACGCA
      121 CGTGGCCATT ATTGGCCATA ATGGTAGCGG CAAAAGCACC CTGGTGAAGC TGCTGGGTGG
      181 TTTCCTGAAA GCCCCTAAAC AGACCATTTT CTTTCGTGGC CAGCCGTTGG AGGAAGTTGG
      241 TTTTCGCCAG ATTGGCATCC TGCTGCAGGA TCCGGATATG CAGCTGTTGG GTGACACGTT
      301 GCATCAGGAG CTGATTTTTA GCCTGGAGAA CCACGGTGTC GATCCTAAAC AGATGGACCG
      361 TGTCATTGCC GACGTTCTGG CAGTCGTCGA GCTGCAAGGC AAACAATTCA CCCCGCTGTC
      421 GAAGCTGAGC TTCGGCGAAA AGCAGCGTGC GGTGTTCGCT TGTCTGCTGG CTGTGAAACC
      481 TGAACTGTAC TTGTTAGACG AGGCATTTAG CATGCTGGAT GGTAAACTGG CGTCAAAATT
      541 GAAGCGCTTC ATTTTTAAAG TCATCAAGGA GCAACAGAAA ACCGTGATTA ACGTTACCCA
      601 CGATTTCAAT GATCTGTTCC TGGCGGATGA GATTATTTTT CTGAGCAAGG GCCAACTGTT
      661 GAAACAATTT AGCCCGGCAG CGATTTATAA ACAACTCCAT TTGTTCCATC AACATCACTT
      721 CACCCTGCCG TTCCCGTGGC TGCTGGCGCA CGAGGTGGCT GATCATCTGC ACCACGAGAT
      781 GAAAGGCCCG ATCGAGGAAC TGCAAGATGT CGTGGACTGG ATTTGTAAGC ATTTGAAGTG
      841 AAGAGCTTAG AGACCCCAGG AAAGTCGGCT AATCTCGCTG AGGTGTCAAT CGTCGGAGCC
      901 GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG
      961 CATGGTCATA GCTGTTTCCT GAGAGCTTGG CAGGTGATGA CACACATTAA CAAATTTCGT
     1021 GAGGAGTCTC CAGAAGAATG CCATTAATTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA
     1081 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC
     1141 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA
     1201 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA
     1261 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA
     1321 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA
     1381 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG
     1441 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG
     1501 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG
     1561 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG
     1621 AAAAAAAGGA TCTCAAGAAG GCCTACTATT AGCAACAACG ATCCTTTGAT CTTTTCTACG
     1681 GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA
     1741 AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT
     1801 ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA
     1861 GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG
     1921 ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA ACCACGCTCA
     1981 CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT
     2041 CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT
     2101 AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA
     2161 CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA
     2221 TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA
     2281 AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT
     2341 GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA
     2401 GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG
     2461 CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC
     2521 TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA
     2581 TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT
     2641 GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT
     2701 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT
     2761 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACCTGCA CCAGTCAGTA AAACGACGGC
     2821 CAGTAGTCAA AAGCCTCCGA CCGGAGGCTT TTGACTTGGT CAGGTGGAGT GGGAG
//