LOCUS Exported 2944 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..2944 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 982..1029 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 2877..2893 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 2894..2925 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 29..910 /label="degV" /note="May bind long-chain fatty acids, such as palmitate, and may play a role in lipid transport or fatty acid metabolism." /translation="MKTAIITDSTASIKEGEIQDVYVLPLQVIINGQDTYRDGKDIDYDRVYQLLKEHPQGLNISTSLPRQADLIELIEAIKDKYDRFVFLPLSKGLSGTYDMIVQAVKPLSTPKKEFVVLETSDIAISLKWLVQEVKALTDTNCPTKAIAEVVDQHKQSIFTAVTVKNLVQLRKGGRISGLKKIIATLLRVKPIIFFDKGVNTLSGKAFTFVQALEKIFTFVKSKFGDNFKIKRIGFCNAFTPVKAKEVKALILDFLHTNKITLQREIESSFITSAIIAHTGIDAFSISLLLDKNK" primer_bind complement(1034..1050) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(1117..1705) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(1896..2756) /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" promoter complement(2757..2861) /label="bla promoter" /note="ampicillin resistance gene promoter" ORIGIN 1 AAATAAGACG ACGACCCTCG GGTCTCAAAT GAAAACGGCC ATTATTACTG ACAGCACGGC 61 GAGCATCAAG GAAGGCGAGA TCCAGGACGT TTACGTTCTC CCGCTGCAAG TCATTATTAA 121 CGGTCAAGAT ACTTATCGTG ATGGTAAAGA CATTGATTAC GACCGTGTCT ACCAACTGCT 181 GAAAGAGCAC CCGCAAGGCC TGAACATTTC CACCAGCCTG CCGCGCCAAG CGGATTTGAT 241 CGAGCTGATC GAGGCTATCA AAGATAAGTA CGACCGTTTC GTGTTCCTCC CTCTGAGCAA 301 GGGCCTGAGC GGTACTTACG ATATGATTGT TCAAGCCGTC AAGCCACTGT CGACGCCGAA 361 GAAGGAATTT GTCGTGCTGG AAACTAGCGA CATTGCTATC AGCTTGAAAT GGCTGGTTCA 421 GGAGGTTAAA GCACTGACGG ATACCAATTG CCCGACCAAG GCGATCGCCG AAGTTGTTGA 481 TCAGCACAAA CAGAGCATCT TCACGGCAGT TACTGTCAAG AACCTGGTGC AGCTGCGCAA 541 AGGTGGCCGC ATCTCTGGTC TGAAGAAGAT TATCGCGACC TTGCTGCGTG TGAAACCGAT 601 TATTTTCTTT GACAAGGGTG TTAACACCCT GTCCGGCAAG GCATTCACCT TCGTCCAGGC 661 CCTGGAAAAA ATTTTTACGT TCGTTAAGAG CAAGTTCGGC GATAACTTTA AGATTAAACG 721 TATTGGTTTC TGCAACGCCT TTACCCCGGT GAAGGCTAAG GAGGTCAAGG CGCTGATTTT 781 GGATTTCCTG CACACCAATA AGATCACCCT CCAACGCGAG ATCGAATCCA GCTTCATCAC 841 CAGCGCGATC ATTGCTCATA CCGGTATTGA TGCCTTCAGC ATCAGCCTGT TGCTGGACAA 901 AAACAAGTGA AGAGCTTAGA GACCCTTCAT TGGTCTGGTG TCCTCGCTGA GGTGTCAATC 961 GTCGGAGCCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG 1021 GGTTTTTTGC ATGGTCATAG CTGTTTCCTG AGAGCTTGGC AGGTGATGAC ACACATTAAC 1081 AAATTTCGTG AGGAGTCTCC AGAAGAATGC CATTAATTTC CATAGGCTCC GCCCCCCTGA 1141 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 1201 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 1261 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 1321 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 1381 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 1441 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 1501 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 1561 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 1621 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 1681 TACGCGCAGA AAAAAAGGAT CTCAAGAAGG CCTACTATTA GCAACAACGA TCCTTTGATC 1741 TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG 1801 AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA 1861 ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA 1921 CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG 1981 ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAA 2041 CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC 2101 AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT 2161 AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC TACAGGCATC 2221 GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT CCGGTTCCCA ACGATCAAGG 2281 CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG TCCTCCGATC 2341 GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG TTATGGCAGC ACTGCATAAT 2401 TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA CTGGTGAGTA CTCAACCAAG 2461 TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT GCCCGGCGTC AATACGGGAT 2521 AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA TTGGAAAACG TTCTTCGGGG 2581 CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT CGATGTAACC CACTCGTGCA 2641 CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA 2701 AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA AATGTTGAAT ACTCATACTC 2761 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 2821 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACCTGCAC CAGTCAGTAA 2881 AACGACGGCC AGTAGTCAAA AGCCTCCGAC CGGAGGCTTT TGACTTGGTC AGGTGGAGTG 2941 GGAG //