LOCUS       Exported     3487 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3487
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1525..1572
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3420..3436
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 for"
     terminator      3437..3468
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..1453
                     /label="MPN491"
                     /note=""
                     /translation="MKLTLWLVLGAVGVGAVGTGVGFGTKYFLDHKAKSNVDVAFKQRSLRKQVNVGFWNALNFLGSERSFGNQNSKVKTKGIAEIINLLKYDIVGLAEIQPSTESTAERFVDQLNEYADPYTSWSYDLSPVTTSKTASEGQKERILIVYNSQSVKMEGQGWFYDNPEMDISNYVSDGSSSKTRTPKKTKTSKKKPIKKKSSKSKSSKGSKKQKTTNESESETLELKLEQRRVTTRSQSKQQKGQEQATDQTDSEGVTTEEGADNTDTELVETTAETTEQEATTKSTKDTKETKDRTKVDWSRPPYSAEFSTQKGKVVVAFGHFDSPGANTKRGEKIAKMGKGQGAHEYFEAQTTFKAMDSIKQHFNNENILFMADTNIKFGNQAAAFGESKDYTFLTEDNEAWKSSLGTKSGYANPYDKIITSNSFKNKVLDQAETYWNYFDRYLKTKGHNSYLFDLASWGTKPRTISDHAPVGVLF"
     primer_bind     complement(1577..1593)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1660..2248)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2439..3299)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3300..3404)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 ACAAGGAGTC GGCATATCAC GGTCTCAAAT GAAACTCACT CTGTGGCTGG TCTTGGGCGC
       61 AGTTGGCGTC GGTGCAGTCG GTACCGGTGT TGGCTTTGGT ACCAAATACT TTCTGGATCA
      121 CAAGGCTAAA AGCAATGTTG ATGTCGCCTT CAAGCAACGT TCTCTGCGCA AACAGGTGAA
      181 CGTTGGTTTC TGGAACGCGT TGAACTTCTT GGGTTCAGAA CGCAGCTTTG GCAATCAAAA
      241 CTCCAAGGTG AAAACCAAAG GTATTGCCGA GATCATCAAT CTGCTGAAGT ACGACATTGT
      301 GGGCCTGGCC GAAATCCAAC CGTCCACGGA GAGCACCGCG GAACGCTTCG TTGACCAGCT
      361 GAATGAATAC GCAGACCCGT ATACGAGCTG GAGCTACGAC CTGAGCCCGG TCACCACGAG
      421 CAAGACCGCA AGCGAGGGTC AAAAAGAACG TATCCTGATT GTTTATAACA GCCAGAGCGT
      481 TAAAATGGAA GGTCAGGGCT GGTTCTACGA TAACCCGGAA ATGGACATTA GCAATTATGT
      541 TTCCGACGGT AGCAGCTCAA AAACGCGCAC GCCGAAAAAG ACCAAGACCT CCAAGAAAAA
      601 GCCGATCAAG AAAAAATCCT CTAAGTCGAA GTCCAGCAAG GGTTCCAAGA AGCAAAAGAC
      661 CACGAATGAA AGCGAGAGCG AAACGCTGGA ATTGAAGCTG GAACAACGCC GCGTGACTAC
      721 CCGTTCTCAA AGCAAGCAGC AAAAAGGCCA AGAGCAGGCG ACGGACCAAA CCGACTCCGA
      781 GGGTGTCACC ACTGAGGAAG GTGCAGACAA CACGGATACC GAACTGGTTG AAACCACCGC
      841 GGAGACTACC GAGCAAGAAG CTACCACCAA GAGCACCAAG GACACGAAGG AAACGAAAGA
      901 TAGAACGAAA GTTGACTGGA GCCGTCCACC GTACTCGGCC GAGTTCTCCA CCCAAAAAGG
      961 TAAGGTGGTA GTGGCCTTCG GTCACTTTGA TAGCCCGGGT GCAAACACCA AACGCGGTGA
     1021 GAAAATCGCT AAGATGGGTA AAGGTCAGGG CGCACACGAG TACTTCGAAG CTCAAACCAC
     1081 CTTTAAAGCA ATGGACTCGA TCAAACAGCA TTTCAATAAC GAAAATATCC TGTTCATGGC
     1141 CGACACCAAC ATCAAGTTTG GTAATCAGGC CGCTGCGTTC GGTGAGAGCA AGGACTATAC
     1201 CTTCCTGACC GAGGACAACG AGGCGTGGAA GTCCAGCCTG GGTACGAAAA GCGGCTATGC
     1261 CAATCCTTAC GACAAAATCA TTACGTCCAA TAGCTTTAAA AATAAGGTGT TGGATCAGGC
     1321 TGAAACCTAC TGGAATTATT TTGACCGTTA CTTAAAGACG AAAGGCCACA ATAGCTACCT
     1381 GTTCGACCTG GCGAGCTGGG GTACCAAACC GCGCACCATT AGCGATCATG CCCCGGTTGG
     1441 TGTCCTCTTT TGAAGAGCTT AGAGACCCTT CATTGGTCTG GTGTCCTCGC TGAGGTGTCA
     1501 ATCGTCGGAG CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG
     1561 AGGGGTTTTT TGCATGGTCA TAGCTGTTTC CTGAGAGCTT GGCAGGTGAT GACACACATT
     1621 AACAAATTTC GTGAGGAGTC TCCAGAAGAA TGCCATTAAT TTCCATAGGC TCCGCCCCCC
     1681 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA
     1741 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC
     1801 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC
     1861 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA
     1921 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC
     1981 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG
     2041 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG
     2101 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG
     2161 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA
     2221 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGGCCTACTA TTAGCAACAA CGATCCTTTG
     2281 ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC
     2341 ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA
     2401 TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT AATCAGTGAG
     2461 GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG TTGCCTGACT CCCCGTCGTG
     2521 TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA GTGCTGCAAT GATACCGCGA
     2581 GAACCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC AGCCAGCCGG AAGGGCCGAG
     2641 CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG TTGCCGGGAA
     2701 GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT TGCTACAGGC
     2761 ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA GCTCCGGTTC CCAACGATCA
     2821 AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG
     2881 ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA TGGTTATGGC AGCACTGCAT
     2941 AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG TGACTGGTGA GTACTCAACC
     3001 AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT CTTGCCCGGC GTCAATACGG
     3061 GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA TCATTGGAAA ACGTTCTTCG
     3121 GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA GTTCGATGTA ACCCACTCGT
     3181 GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG TTTCTGGGTG AGCAAAAACA
     3241 GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC GGAAATGTTG AATACTCATA
     3301 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC
     3361 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACCTG CACCAGTCAG
     3421 TAAAACGACG GCCAGTAGTC AAAAGCCTCC GACCGGAGGC TTTTGACTTG GTCAGGTGGA
     3481 GTGGGAG
//