LOCUS       Exported     2653 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..2653
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      691..738
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     2586..2602
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 for"
     terminator      2603..2634
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..619
                     /label="MPN501"
                     /note=""
                     /translation="MKEKIPFYNEKEFHDMVKKTKKGTFSGWYIIDKDNNSVEFSGKFNRQFKLNKPVIPVNTEYVTRKEFNEYKNSNDQRLTKIETTLAAQGEQINKLTQTVEKQGEQINQLVQVVLLHGEQINKLTQTVEKQGEQIKELQIEQKAQGETLKLILQTLQKMSDRLDKMEVKMDKMEVKMDKMEVKMDKMEKRIDKLESK"
     primer_bind     complement(743..759)
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 rev"
     rep_origin      complement(826..1414)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(1605..2465)
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
     promoter        complement(2466..2570)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 ACAACGAGCA GACCGAATAG GGTCTCAAAT GAAGGAGAAA ATCCCGTTCT ATAACGAAAA
       61 AGAGTTCCAC GATATGGTCA AAAAGACGAA GAAAGGCACC TTCAGCGGTT GGTACATCAT
      121 CGATAAAGAT AATAATAGCG TTGAGTTCAG CGGCAAATTT AATCGTCAAT TCAAGCTGAA
      181 CAAGCCTGTT ATTCCGGTCA ATACGGAGTA CGTTACCCGT AAGGAGTTCA ACGAGTACAA
      241 AAACAGCAAT GACCAACGCC TGACGAAGAT CGAAACCACC CTGGCGGCGC AGGGTGAGCA
      301 AATTAATAAA CTGACCCAGA CGGTGGAAAA ACAAGGCGAG CAGATCAACC AGCTGGTTCA
      361 AGTTGTCCTG CTCCATGGTG AGCAAATCAA TAAGCTCACC CAGACCGTCG AAAAACAGGG
      421 CGAGCAGATT AAGGAGCTGC AAATTGAGCA AAAAGCACAA GGCGAAACGC TGAAACTGAT
      481 CCTGCAAACC CTGCAGAAAA TGAGCGACCG TTTGGACAAA ATGGAGGTTA AGATGGATAA
      541 AATGGAGGTG AAGATGGACA AGATGGAGGT TAAAATGGAC AAAATGGAAA AACGTATCGA
      601 CAAGCTGGAA TCTAAGTGAA GAGCTTAGAG ACCCCATCTT GCGTCGTGAG AATCGCTGAG
      661 GTGTCAATCG TCGGAGCCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG
      721 GTCTTGAGGG GTTTTTTGCA TGGTCATAGC TGTTTCCTGA GAGCTTGGCA GGTGATGACA
      781 CACATTAACA AATTTCGTGA GGAGTCTCCA GAAGAATGCC ATTAATTTCC ATAGGCTCCG
      841 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG
      901 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC
      961 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA
     1021 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT
     1081 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC
     1141 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG
     1201 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC
     1261 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT
     1321 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA
     1381 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGGC CTACTATTAG CAACAACGAT
     1441 CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT
     1501 TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT
     1561 TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC
     1621 AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC
     1681 GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA
     1741 CCGCGAGAAC CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG
     1801 GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC
     1861 CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT
     1921 ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA
     1981 CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT
     2041 CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA
     2101 CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC
     2161 TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA
     2221 ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT
     2281 TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC
     2341 ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA
     2401 AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA
     2461 CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC
     2521 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACCTGCACC
     2581 AGTCAGTAAA ACGACGGCCA GTAGTCAAAA GCCTCCGACC GGAGGCTTTT GACTTGGTCA
     2641 GGTGGAGTGG GAG
//