LOCUS       Exported     3235 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3235
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1273..1320
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3168..3184
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3185..3216
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..1201
                     /note="Catalyzes the formation of acetyl phosphate from acetate and ATP. Can also catalyze the reverse reaction."
                     /translation="MNDNKILVVNAGSSSIKFQLFDYHKKVLAKALCERIFVDGFFKLEFNEQKVEEKVAFPDHHAAVTHFLNTLKKHKIIQELSDIILVGHRVVQGANYFKDSVIVDAEALAKIKEFIKLAPLHNKPEADVIEIFFKEVPSAKNVAVFDTTFHTTIPQENYLYAVPRSWEQKHLVRRYGFHGTSYKFINNYLEKHLNKQNLNLIVCHLGNGASVCAIKNGKSFNTSMGFTPLEGLIMGTRSGDLDPAIIGYVAEQENMSASDVVNALNKKSGMLALTGASDMRDVFAKPQENAVAIKMYVNRVADYIAKYLNQLEGNIDGLVFTGGIGENASDCVELFINAVKSLGFATDLKLFVKYGDSCVVSTPQSKYKIYRVRTNEELMIVEDSIRLTQK"
                     /label="ackA"
     primer_bind     complement(1325..1341)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1408..1996)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2187..3047)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3048..3152)
                     /note="ampicillin resistance gene promoter"
                     /label="bla promoter"
ORIGIN
        1 AAAGCACTCT TAGGCCTCTG GGTCTCAAAT GAATGACAAC AAGATCCTGG TGGTGAACGC
       61 GGGTAGCAGC AGCATTAAGT TCCAACTGTT TGATTATCAT AAAAAAGTCT TGGCGAAAGC
      121 TCTGTGTGAA CGCATTTTCG TTGACGGCTT CTTCAAACTG GAATTCAACG AACAAAAAGT
      181 GGAAGAAAAG GTGGCTTTCC CGGATCACCA CGCAGCAGTC ACTCACTTTC TGAATACCCT
      241 TAAGAAGCAC AAAATTATTC AGGAATTGAG CGACATCATT CTGGTGGGCC ACCGCGTGGT
      301 TCAGGGTGCG AATTATTTCA AAGATAGCGT TATCGTGGAC GCGGAAGCTC TGGCGAAAAT
      361 TAAAGAGTTC ATCAAACTGG CACCGCTGCA TAATAAACCG GAGGCAGATG TGATCGAAAT
      421 CTTTTTCAAG GAGGTTCCGA GCGCCAAGAA CGTTGCGGTG TTCGATACGA CGTTCCATAC
      481 CACCATTCCG CAAGAGAACT ACCTGTACGC GGTTCCACGT AGCTGGGAGC AGAAGCACCT
      541 GGTGCGCCGT TACGGTTTTC ACGGTACCAG CTACAAGTTT ATCAATAATT ATCTGGAAAA
      601 GCACCTGAAT AAGCAGAACC TGAACCTGAT CGTTTGTCAC CTGGGTAATG GTGCAAGCGT
      661 GTGCGCAATC AAGAATGGTA AAAGCTTCAA TACGTCTATG GGTTTCACGC CGCTGGAAGG
      721 TCTGATCATG GGTACGCGTT CTGGTGATCT GGACCCGGCG ATTATCGGCT ATGTCGCGGA
      781 GCAGGAGAAC ATGAGCGCAA GCGACGTCGT TAATGCGCTG AACAAGAAAA GCGGTATGCT
      841 CGCGTTGACG GGTGCCAGCG ACATGCGCGA CGTTTTCGCT AAACCGCAAG AGAACGCAGT
      901 CGCTATTAAA ATGTACGTGA ATAGAGTTGC GGACTACATT GCTAAGTACC TGAACCAACT
      961 GGAGGGTAAC ATCGACGGCC TGGTGTTCAC GGGCGGTATC GGCGAAAACG CATCGGACTG
     1021 CGTCGAATTG TTCATCAACG CAGTTAAAAG CCTGGGCTTC GCGACCGACC TGAAGCTGTT
     1081 TGTTAAGTAT GGTGACAGCT GTGTGGTCAG CACCCCGCAA TCCAAGTACA AGATTTACCG
     1141 TGTCCGTACC AACGAGGAAC TGATGATCGT CGAGGACAGC ATCCGCCTGA CGCAAAAGTG
     1201 AAGAGCTTAG AGACCCGGAT TCTAGGTGCA ACTCTCGCTG AGGTGTCAAT CGTCGGAGCC
     1261 GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG
     1321 CATGGTCATA GCTGTTTCCT GAGAGCTTGG CAGGTGATGA CACACATTAA CAAATTTCGT
     1381 GAGGAGTCTC CAGAAGAATG CCATTAATTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA
     1441 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC
     1501 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA
     1561 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA
     1621 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA
     1681 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA
     1741 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG
     1801 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG
     1861 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG
     1921 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG
     1981 AAAAAAAGGA TCTCAAGAAG GCCTACTATT AGCAACAACG ATCCTTTGAT CTTTTCTACG
     2041 GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA
     2101 AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT
     2161 ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA
     2221 GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG
     2281 ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA ACCACGCTCA
     2341 CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT
     2401 CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT
     2461 AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA
     2521 CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA
     2581 TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA
     2641 AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT
     2701 GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA
     2761 GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG
     2821 CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC
     2881 TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA
     2941 TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT
     3001 GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT
     3061 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT
     3121 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACCTGCA CCAGTCAGTA AAACGACGGC
     3181 CAGTAGTCAA AAGCCTCCGA CCGGAGGCTT TTGACTTGGT CAGGTGGAGT GGGAG
//