LOCUS       Exported     3049 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3049
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1087..1134
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     2982..2998
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      2999..3030
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..1015
                     /label="plsX"
                     /note="Catalyzes the reversible formation of acyl-phosphate (acyl-PO(4)) from acyl-[acyl-carrier-protein] (acyl-ACP). This enzyme utilizes acyl-ACP as fatty acyl donor, but not acyl-CoA."
                     /translation="MAFRLAVDCLGFENHPREAIDAVLEYWSYHQELEFILVGDEKTFDGLDYLPKNITKQLASSCIDMTDTPLTARRKVNNSMQKAINLVRDGAADVVISAGSSAVYASLTYDGFGKIHKDVKSAFMSYVPTANNDWFYFLDVGANKNFTGKELYFLGLMADIFVKKTTNKISPRIALLNIGTEIHKGFDYHQEGYQLLNEDKHLNFTGFIEPRFLLDGVCDILVADGYSGNLVLKSMEGTFKTIARLLKQGYKRNPLAGLFSLGILKRIAKRFDYKNNAGAVVIGLNKLALKTHGSADKQQFLSTIRLAHTSLKSDLINAIKSSLDNYEK"
     primer_bind     complement(1139..1155)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1222..1810)
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
                     /label="ori"
     CDS             complement(2001..2861)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(2862..2966)
                     /note="ampicillin resistance gene promoter"
                     /label="bla promoter"
ORIGIN
        1 AATCAGTTTC TTTGGCAGCC GGTCTCAAAT GGCGTTCCGC CTCGCCGTTG ACTGCCTGGG
       61 TTTCGAAAAC CACCCGCGTG AGGCCATCGA CGCTGTTCTG GAATATTGGA GCTACCACCA
      121 GGAACTGGAG TTCATCCTGG TGGGTGATGA AAAGACGTTC GACGGTCTGG ATTACTTGCC
      181 GAAGAACATC ACGAAACAGC TGGCGTCTAG CTGCATCGAT ATGACTGACA CCCCGCTGAC
      241 CGCTCGTCGC AAGGTCAATA ACAGCATGCA AAAAGCGATT AATTTGGTCC GCGACGGTGC
      301 CGCTGACGTA GTGATTAGCG CCGGTTCGAG CGCGGTGTAT GCCAGCTTGA CCTACGATGG
      361 CTTTGGCAAG ATCCATAAAG ACGTGAAAAG CGCTTTTATG AGCTATGTCC CAACCGCCAA
      421 CAATGATTGG TTCTACTTTC TGGACGTGGG CGCAAACAAG AATTTTACCG GTAAGGAACT
      481 GTATTTTTTG GGTCTGATGG CGGATATCTT CGTCAAGAAA ACGACCAATA AAATTAGCCC
      541 GCGCATTGCC CTGCTTAACA TCGGCACGGA GATCCACAAG GGCTTTGATT ATCATCAGGA
      601 GGGTTATCAG CTGCTGAACG AGGACAAACA CCTGAATTTT ACCGGCTTCA TTGAGCCGCG
      661 CTTTCTGTTG GACGGTGTTT GCGACATTCT GGTGGCCGAT GGTTACTCCG GTAATCTGGT
      721 CCTGAAGTCC ATGGAGGGTA CCTTCAAAAC TATCGCGCGC CTGCTGAAGC AGGGTTATAA
      781 GCGTAATCCT CTGGCAGGCC TGTTTTCGCT GGGTATTCTG AAACGTATCG CAAAGCGTTT
      841 TGATTACAAA AACAACGCGG GCGCGGTGGT TATCGGTCTG AATAAGTTGG CTCTCAAGAC
      901 CCATGGTTCA GCTGATAAAC AACAGTTCCT GAGCACCATT CGCCTGGCGC ACACCAGCTT
      961 AAAGAGCGAC CTGATCAACG CGATCAAAAG CAGCTTGGAC AATTATGAGA AGTGAAGAGC
     1021 TTAGAGACCG CCATCCTCTT ATCTCGTGGC GCTGAGGTGT CAATCGTCGG AGCCGCTGAG
     1081 CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCATGGT
     1141 CATAGCTGTT TCCTGAGAGC TTGGCAGGTG ATGACACACA TTAACAAATT TCGTGAGGAG
     1201 TCTCCAGAAG AATGCCATTA ATTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA
     1261 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC
     1321 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC
     1381 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG
     1441 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA
     1501 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC
     1561 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC
     1621 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG
     1681 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA
     1741 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA
     1801 AGGATCTCAA GAAGGCCTAC TATTAGCAAC AACGATCCTT TGATCTTTTC TACGGGGTCT
     1861 GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG
     1921 ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT
     1981 GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC
     2041 TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG
     2101 GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGAACCACG CTCACCGGCT
     2161 CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA
     2221 ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG
     2281 CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG
     2341 TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC
     2401 CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG
     2461 TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG
     2521 CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG
     2581 TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT
     2641 AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG
     2701 ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA
     2761 GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA
     2821 AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT
     2881 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG
     2941 AAAAATAAAC AAATAGGGGT TCCGCGCACC TGCACCAGTC AGTAAAACGA CGGCCAGTAG
     3001 TCAAAAGCCT CCGACCGGAG GCTTTTGACT TGGTCAGGTG GAGTGGGAG
//