LOCUS       Exported     3757 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3757
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1795..1842
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3690..3706
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 for"
     terminator      3707..3738
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..1723
                     /label="thrSv"
                     /note="Catalyzes the attachment of threonine to tRNA(Thr) in a two-step reaction: L-threonine is first activated by ATP to form Thr-AMP and then transferred to the acceptor end of tRNA(Thr). Also edits incorrectly charged L-seryl-tRNA(Thr)."
                     /translation="MLAGVLLLQSWLKANYPQVQFGSYGVQEGEFYLDFKVEKSFSIKEFEQLEQDLNAYFNQLNNVVQTKIDKTKSLKFFQADPFTTTLIESIESNSLVVTTVNEQTFWVHDLVLPLPKQGFVKLLNVSANYFLGDPTKPQLQRLVGIFAASKEQLKSLIAANEQKYQNDHRAIGKRLEIFTFDPLVGAGFPIWLEKGAVLKKIIGDFVHYQQLMFGFKTVSSPVLANLELYKISGHYVHYSEDMFPSVKLENQMMMLRPMTCPHHCLIFKHKRRSYKELPQRFCEDSILHRFEASGGLTGLERVRCMTLLDNHIFCRTDQIKAEIKNAFKLIQTVNNKFGFSFDRIDLALHDPNNKAKFIDNDQLWANSESQIESVFKELNVPYKIDVGAAAFYGPKIDFQFKTALNKMITIATIQLDFLLPERFELRYFDEQSNAQTPVIIHVGIVGTYERFIAALLEKTHGNLPLWMAPVQVVVIPVNIDKHEKAAKKLYQKLLKENIRVSLDESEDRLGKKVRTAIVNKIPLQIIVGDKEMQDLTRITCRGFKGEKVQKLYWANFVKKVRKDG"
     primer_bind     complement(1847..1863)
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 rev"
     rep_origin      complement(1930..2518)
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
                     /label="ori"
     CDS             complement(2709..3569)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3570..3674)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 AATGCAAAGC TATTAGCGCG GGTCTCAAAT GCTGGCTGGC GTGCTGCTGC TGCAGTCCTG
       61 GCTCAAAGCG AATTATCCTC AGGTCCAATT CGGTAGCTAC GGTGTTCAGG AGGGTGAGTT
      121 CTACCTGGAT TTTAAAGTCG AGAAATCGTT CAGCATTAAA GAGTTCGAAC AGCTGGAGCA
      181 GGATCTGAAC GCCTACTTCA ACCAGCTGAA TAATGTTGTT CAAACCAAGA TTGACAAGAC
      241 CAAGTCCCTG AAATTTTTCC AGGCTGACCC GTTCACGACC ACCCTGATCG AAAGCATTGA
      301 GTCCAACTCA TTAGTGGTTA CCACCGTGAA CGAGCAAACC TTTTGGGTCC ACGACCTGGT
      361 TCTGCCGTTG CCGAAGCAAG GTTTCGTGAA GCTGCTGAAC GTGAGCGCAA ACTACTTTCT
      421 GGGTGACCCG ACCAAGCCTC AACTGCAACG TCTGGTCGGT ATCTTTGCGG CAAGCAAAGA
      481 ACAACTGAAA TCTCTGATTG CGGCTAACGA ACAGAAATAC CAGAACGATC ACCGTGCGAT
      541 TGGTAAGCGT TTGGAGATCT TCACCTTTGA TCCGCTGGTG GGTGCCGGTT TTCCTATCTG
      601 GCTGGAAAAA GGCGCAGTCC TTAAGAAAAT CATTGGCGAC TTTGTTCACT ACCAACAGCT
      661 GATGTTCGGT TTCAAAACGG TTAGCTCTCC AGTCTTAGCG AACCTGGAAC TGTACAAGAT
      721 TAGCGGCCAT TACGTTCATT ATAGCGAGGA TATGTTTCCA AGCGTTAAAT TGGAAAACCA
      781 GATGATGATG TTGCGCCCGA TGACGTGCCC GCACCACTGT CTGATTTTTA AACATAAGCG
      841 TCGTAGCTAC AAGGAACTGC CGCAACGTTT TTGCGAGGAC AGCATTCTGC ATCGTTTTGA
      901 GGCCTCCGGT GGCCTCACGG GTCTGGAGCG TGTTCGTTGC ATGACTCTGT TGGACAATCA
      961 TATTTTCTGT CGCACCGATC AAATCAAGGC AGAAATTAAA AATGCATTCA AGCTGATTCA
     1021 AACCGTCAAT AACAAGTTCG GCTTCAGCTT TGATCGTATT GACCTGGCCC TGCATGACCC
     1081 AAATAATAAG GCCAAATTTA TTGACAATGA CCAGCTGTGG GCAAATAGCG AATCTCAAAT
     1141 TGAAAGCGTG TTCAAGGAGC TGAATGTTCC GTACAAGATT GATGTCGGTG CTGCGGCGTT
     1201 CTACGGTCCG AAAATTGATT TTCAGTTTAA GACGGCCCTG AACAAGATGA TTACGATCGC
     1261 AACCATTCAG CTGGATTTTC TGCTGCCGGA GCGTTTCGAG CTGCGTTACT TTGATGAACA
     1321 GAGCAATGCC CAAACGCCGG TCATCATTCA CGTTGGCATT GTGGGCACGT ATGAGCGCTT
     1381 TATTGCGGCG CTGTTGGAGA AAACCCACGG TAATCTGCCG CTGTGGATGG CCCCAGTCCA
     1441 GGTCGTTGTG ATTCCTGTAA ATATTGATAA GCACGAGAAG GCGGCAAAAA AGCTGTATCA
     1501 GAAACTGTTG AAAGAGAACA TTCGCGTTAG CCTGGACGAA AGCGAGGACC GTCTGGGTAA
     1561 GAAAGTGCGC ACGGCAATTG TTAACAAGAT CCCGCTGCAG ATTATCGTTG GTGACAAGGA
     1621 AATGCAGGAC CTCACGCGTA TTACGTGCCG CGGTTTTAAG GGTGAAAAAG TTCAAAAGCT
     1681 GTACTGGGCG AATTTCGTCA AAAAGGTTCG TAAGGACGGC TGAAGAGCTT AGAGACCGTA
     1741 TGTCCCCTAA GCTCTGGCGC TGAGGTGTCA ATCGTCGGAG CCGCTGAGCA ATAACTAGCA
     1801 TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCATGGTCA TAGCTGTTTC
     1861 CTGAGAGCTT GGCAGGTGAT GACACACATT AACAAATTTC GTGAGGAGTC TCCAGAAGAA
     1921 TGCCATTAAT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG
     1981 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC
     2041 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC
     2101 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT
     2161 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT
     2221 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC
     2281 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA
     2341 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA
     2401 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG
     2461 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA
     2521 AGGCCTACTA TTAGCAACAA CGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG
     2581 AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG
     2641 ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG
     2701 TCTGACAGTT ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT
     2761 TCATCCATAG TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA
     2821 TCTGGCCCCA GTGCTGCAAT GATACCGCGA GAACCACGCT CACCGGCTCC AGATTTATCA
     2881 GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC
     2941 TCCATCCAGT CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT
     3001 TTGCGCAACG TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG
     3061 GCTTCATTCA GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC
     3121 AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG
     3181 TTATCACTCA TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA
     3241 TGCTTTTCTG TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA
     3301 CCGAGTTGCT CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA
     3361 AAAGTGCTCA TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG
     3421 TTGAGATCCA GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT
     3481 TTCACCAGCG TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA
     3541 AGGGCGACAC GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT
     3601 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA
     3661 ATAGGGGTTC CGCGCACCTG CACCAGTCAG TAAAACGACG GCCAGTAGTC AAAAGCCTCC
     3721 GACCGGAGGC TTTTGACTTG GTCAGGTGGA GTGGGAG
//