LOCUS Exported 3676 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..3676 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 1714..1761 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 3609..3625 /note="common sequencing primer, one of multiple similar variants" /label="M13 for" terminator 3626..3657 /note="bidirectional E. coli tonB-P14 transcription terminator" /label="tonB terminator" CDS 29..1642 /note="" /translation="MLFINTDLQECLNALNLEFDEHKELVKLVKNNSFSGFASTVVFHLKGVNQKETAQQIAAWLLKHKKAHYRRVFVANNNFINFEISPQKYLDFLKTKPTFAPKPTKVLIEWVSANPTGELHLGHVRNAFFGHVLNNLMVFLGFQTVREYWVNDYGQQARVFGFSVYQALHLQQNIKVTPHPDGYEGELVDSIAKTITGIPLDKLSFEEFLQQPFLDQLLADCTAKVLEVIKQDLATIHIHFDSWKFESQVVKETDYKKLLTQFKDEAHYEKDGAIWLKTTLYGDDKDRVLVRQDNRPSYFGTDVAYHLDKAARGFDLLYDIWGSDHEGHIKRMHCVYEGLKIHQKCQLKITALQLVMLYKNKEIVRLSKRAGNVITIKQMLQMLSEDAARWFMLSQTNNSIIKIDLDTANLQNSSNPVYYVQYAYARMCSVLKVVDQAALAQVNDCSLLTHEKEIALLDQLVYFKSLLEKVQVSHELHLLTNYLYETATLFHSWYKACKINDPAQYNLTQQRLLLLQSLHHVFGQLLQILNITAPQQM" /label="argS" primer_bind complement(1766..1782) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(1849..2437) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(2628..3488) /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" promoter complement(3489..3593) /label="bla promoter" /note="ampicillin resistance gene promoter" ORIGIN 1 AATATCACGC AAAAGCACCG GGTCTCAAAT GCTGTTTATT AACACCGACC TTCAGGAGTG 61 TCTGAACGCA CTGAACCTGG AATTCGACGA GCACAAGGAG TTGGTGAAGC TGGTGAAGAA 121 TAACTCCTTT TCTGGCTTTG CGAGCACCGT TGTTTTCCAC TTGAAAGGCG TTAATCAGAA 181 AGAAACCGCA CAGCAGATTG CCGCCTGGCT TCTGAAACAT AAGAAAGCGC ACTATCGTAG 241 AGTGTTTGTG GCAAATAATA ATTTCATCAA CTTTGAGATT TCTCCGCAAA AGTACCTCGA 301 CTTTCTTAAG ACCAAACCTA CTTTCGCTCC GAAACCGACC AAGGTTCTGA TTGAGTGGGT 361 TTCTGCGAAT CCGACGGGTG AGCTGCATTT GGGTCACGTG CGTAACGCGT TTTTTGGCCA 421 CGTCCTGAAT AATCTTATGG TGTTCCTGGG CTTCCAGACC GTACGCGAAT ATTGGGTGAA 481 TGACTACGGT CAGCAAGCAC GTGTGTTCGG CTTTAGCGTT TACCAAGCAC TGCATCTGCA 541 GCAGAACATT AAAGTTACCC CTCACCCGGA TGGCTATGAA GGTGAGCTGG TGGATTCGAT 601 CGCGAAAACG ATTACCGGCA TCCCGCTGGA CAAGCTGTCC TTCGAAGAGT TCCTGCAGCA 661 GCCGTTCCTG GACCAGCTGC TCGCAGATTG TACGGCGAAG GTGCTGGAGG TCATCAAGCA 721 AGATCTGGCG ACGATCCACA TTCACTTCGA CAGCTGGAAA TTCGAAAGCC AAGTCGTCAA 781 GGAAACGGAC TACAAAAAAC TGCTGACGCA ATTCAAAGAC GAGGCACACT ACGAGAAGGA 841 CGGTGCTATC TGGTTAAAAA CCACGTTGTA CGGCGACGAT AAAGACCGTG TGCTGGTCCG 901 TCAAGACAAC CGCCCGAGCT ATTTTGGTAC CGACGTCGCG TACCACTTGG ACAAAGCAGC 961 CCGTGGTTTC GATCTGCTGT ATGACATTTG GGGTAGCGAT CATGAGGGCC ACATTAAGCG 1021 TATGCATTGC GTTTATGAGG GCCTCAAAAT TCACCAAAAG TGCCAATTGA AAATCACCGC 1081 GTTGCAGCTG GTCATGCTGT ATAAGAACAA AGAAATTGTC CGCCTGAGCA AACGTGCTGG 1141 CAATGTCATC ACCATCAAGC AAATGCTGCA GATGCTGTCC GAGGACGCGG CCCGCTGGTT 1201 TATGCTGTCG CAGACGAACA ATAGCATCAT TAAGATTGAC CTGGACACGG CAAACTTGCA 1261 AAATAGCAGC AACCCGGTGT ACTACGTCCA GTACGCGTAT GCACGCATGT GCAGCGTGCT 1321 GAAAGTGGTT GACCAAGCAG CGCTGGCCCA AGTTAACGAT TGCAGCTTGC TGACGCACGA 1381 AAAAGAGATC GCGTTGCTGG ATCAGCTGGT GTACTTTAAA AGCCTCCTGG AAAAAGTCCA 1441 AGTTAGCCAC GAACTGCATC TGCTGACGAA TTATCTGTAC GAAACCGCCA CCCTGTTTCA 1501 TAGCTGGTAC AAAGCGTGCA AGATCAACGA TCCAGCTCAG TATAATCTCA CCCAGCAACG 1561 CCTGCTGCTG CTGCAGAGCC TGCATCACGT CTTTGGCCAG CTGCTGCAAA TTTTGAATAT 1621 TACCGCCCCG CAACAAATGT GAAGAGCTTA GAGACCGCCA TCCTCTTATC TCGTGGCGCT 1681 GAGGTGTCAA TCGTCGGAGC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA 1741 CGGGTCTTGA GGGGTTTTTT GCATGGTCAT AGCTGTTTCC TGAGAGCTTG GCAGGTGATG 1801 ACACACATTA ACAAATTTCG TGAGGAGTCT CCAGAAGAAT GCCATTAATT TCCATAGGCT 1861 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 1921 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 1981 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 2041 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 2101 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 2161 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 2221 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 2281 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 2341 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 2401 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GGCCTACTAT TAGCAACAAC 2461 GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG 2521 ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA 2581 AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGCTTA 2641 ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC 2701 CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG 2761 ATACCGCGAG AACCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA 2821 AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT 2881 TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT 2941 GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC 3001 CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC 3061 GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA 3121 GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG 3181 TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG 3241 TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA 3301 CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA 3361 CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA 3421 GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA 3481 ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 3541 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACCTGC 3601 ACCAGTCAGT AAAACGACGG CCAGTAGTCA AAAGCCTCCG ACCGGAGGCT TTTGACTTGG 3661 TCAGGTGGAG TGGGAG //