LOCUS       Exported     3676 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3676
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1714..1761
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3609..3625
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 for"
     terminator      3626..3657
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
                     /label="tonB terminator"
     CDS             29..1642
                     /note=""
                     /translation="MLFINTDLQECLNALNLEFDEHKELVKLVKNNSFSGFASTVVFHLKGVNQKETAQQIAAWLLKHKKAHYRRVFVANNNFINFEISPQKYLDFLKTKPTFAPKPTKVLIEWVSANPTGELHLGHVRNAFFGHVLNNLMVFLGFQTVREYWVNDYGQQARVFGFSVYQALHLQQNIKVTPHPDGYEGELVDSIAKTITGIPLDKLSFEEFLQQPFLDQLLADCTAKVLEVIKQDLATIHIHFDSWKFESQVVKETDYKKLLTQFKDEAHYEKDGAIWLKTTLYGDDKDRVLVRQDNRPSYFGTDVAYHLDKAARGFDLLYDIWGSDHEGHIKRMHCVYEGLKIHQKCQLKITALQLVMLYKNKEIVRLSKRAGNVITIKQMLQMLSEDAARWFMLSQTNNSIIKIDLDTANLQNSSNPVYYVQYAYARMCSVLKVVDQAALAQVNDCSLLTHEKEIALLDQLVYFKSLLEKVQVSHELHLLTNYLYETATLFHSWYKACKINDPAQYNLTQQRLLLLQSLHHVFGQLLQILNITAPQQM"
                     /label="argS"
     primer_bind     complement(1766..1782)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1849..2437)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2628..3488)
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
     promoter        complement(3489..3593)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 AATATCACGC AAAAGCACCG GGTCTCAAAT GCTGTTTATT AACACCGACC TTCAGGAGTG
       61 TCTGAACGCA CTGAACCTGG AATTCGACGA GCACAAGGAG TTGGTGAAGC TGGTGAAGAA
      121 TAACTCCTTT TCTGGCTTTG CGAGCACCGT TGTTTTCCAC TTGAAAGGCG TTAATCAGAA
      181 AGAAACCGCA CAGCAGATTG CCGCCTGGCT TCTGAAACAT AAGAAAGCGC ACTATCGTAG
      241 AGTGTTTGTG GCAAATAATA ATTTCATCAA CTTTGAGATT TCTCCGCAAA AGTACCTCGA
      301 CTTTCTTAAG ACCAAACCTA CTTTCGCTCC GAAACCGACC AAGGTTCTGA TTGAGTGGGT
      361 TTCTGCGAAT CCGACGGGTG AGCTGCATTT GGGTCACGTG CGTAACGCGT TTTTTGGCCA
      421 CGTCCTGAAT AATCTTATGG TGTTCCTGGG CTTCCAGACC GTACGCGAAT ATTGGGTGAA
      481 TGACTACGGT CAGCAAGCAC GTGTGTTCGG CTTTAGCGTT TACCAAGCAC TGCATCTGCA
      541 GCAGAACATT AAAGTTACCC CTCACCCGGA TGGCTATGAA GGTGAGCTGG TGGATTCGAT
      601 CGCGAAAACG ATTACCGGCA TCCCGCTGGA CAAGCTGTCC TTCGAAGAGT TCCTGCAGCA
      661 GCCGTTCCTG GACCAGCTGC TCGCAGATTG TACGGCGAAG GTGCTGGAGG TCATCAAGCA
      721 AGATCTGGCG ACGATCCACA TTCACTTCGA CAGCTGGAAA TTCGAAAGCC AAGTCGTCAA
      781 GGAAACGGAC TACAAAAAAC TGCTGACGCA ATTCAAAGAC GAGGCACACT ACGAGAAGGA
      841 CGGTGCTATC TGGTTAAAAA CCACGTTGTA CGGCGACGAT AAAGACCGTG TGCTGGTCCG
      901 TCAAGACAAC CGCCCGAGCT ATTTTGGTAC CGACGTCGCG TACCACTTGG ACAAAGCAGC
      961 CCGTGGTTTC GATCTGCTGT ATGACATTTG GGGTAGCGAT CATGAGGGCC ACATTAAGCG
     1021 TATGCATTGC GTTTATGAGG GCCTCAAAAT TCACCAAAAG TGCCAATTGA AAATCACCGC
     1081 GTTGCAGCTG GTCATGCTGT ATAAGAACAA AGAAATTGTC CGCCTGAGCA AACGTGCTGG
     1141 CAATGTCATC ACCATCAAGC AAATGCTGCA GATGCTGTCC GAGGACGCGG CCCGCTGGTT
     1201 TATGCTGTCG CAGACGAACA ATAGCATCAT TAAGATTGAC CTGGACACGG CAAACTTGCA
     1261 AAATAGCAGC AACCCGGTGT ACTACGTCCA GTACGCGTAT GCACGCATGT GCAGCGTGCT
     1321 GAAAGTGGTT GACCAAGCAG CGCTGGCCCA AGTTAACGAT TGCAGCTTGC TGACGCACGA
     1381 AAAAGAGATC GCGTTGCTGG ATCAGCTGGT GTACTTTAAA AGCCTCCTGG AAAAAGTCCA
     1441 AGTTAGCCAC GAACTGCATC TGCTGACGAA TTATCTGTAC GAAACCGCCA CCCTGTTTCA
     1501 TAGCTGGTAC AAAGCGTGCA AGATCAACGA TCCAGCTCAG TATAATCTCA CCCAGCAACG
     1561 CCTGCTGCTG CTGCAGAGCC TGCATCACGT CTTTGGCCAG CTGCTGCAAA TTTTGAATAT
     1621 TACCGCCCCG CAACAAATGT GAAGAGCTTA GAGACCGCCA TCCTCTTATC TCGTGGCGCT
     1681 GAGGTGTCAA TCGTCGGAGC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA
     1741 CGGGTCTTGA GGGGTTTTTT GCATGGTCAT AGCTGTTTCC TGAGAGCTTG GCAGGTGATG
     1801 ACACACATTA ACAAATTTCG TGAGGAGTCT CCAGAAGAAT GCCATTAATT TCCATAGGCT
     1861 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC
     1921 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC
     1981 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC
     2041 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG
     2101 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA
     2161 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG
     2221 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA
     2281 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG
     2341 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG
     2401 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GGCCTACTAT TAGCAACAAC
     2461 GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG
     2521 ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA
     2581 AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGCTTA
     2641 ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC
     2701 CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG
     2761 ATACCGCGAG AACCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA
     2821 AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT
     2881 TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT
     2941 GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC
     3001 CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC
     3061 GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA
     3121 GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG
     3181 TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG
     3241 TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA
     3301 CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA
     3361 CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA
     3421 GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA
     3481 ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG
     3541 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACCTGC
     3601 ACCAGTCAGT AAAACGACGG CCAGTAGTCA AAAGCCTCCG ACCGGAGGCT TTTGACTTGG
     3661 TCAGGTGGAG TGGGAG
//