LOCUS Exported 2704 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..2704 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 742..789 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 2637..2653 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 2654..2685 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 29..670 /label="udk" /note="" /translation="MDSKKGILVAIGGGSCSGKTTIADMIYQLLRKKLKVAILPQDNYYKPYKNKSMAQRKAINFDHPDAFDWKLLWSHLDNLLMGKTVAVPMYDYVNYTRKKETIEIGPLNVVILEGLMPWFDERIAKLCKLKIFVEATGEERLIRRIERDWERGRDVASIIKQWREAVSPMYEIFVEKMKQKADLIIPWSERHEVSTNVLDFAIEHLFRKHVDPN" primer_bind complement(794..810) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(877..1465) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(1656..2516) /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" /label="bla" promoter complement(2517..2621) /label="bla promoter" /note="ampicillin resistance gene promoter" ORIGIN 1 AAAGGGGCCG TCAATATCAG GGTCTCAAAT GGACTCGAAA AAAGGTATTC TGGTCGCGAT 61 CGGTGGTGGC AGCTGCAGCG GTAAGACCAC GATCGCGGAC ATGATCTACC AGCTGCTGCG 121 TAAGAAACTG AAAGTTGCCA TTCTGCCGCA GGATAATTAT TATAAACCAT ACAAAAACAA 181 GTCTATGGCA CAACGTAAGG CAATTAACTT CGATCACCCG GATGCGTTTG ATTGGAAATT 241 GTTGTGGAGC CACCTGGACA ATCTGCTGAT GGGCAAAACC GTGGCGGTGC CGATGTACGA 301 TTATGTAAAC TACACTCGCA AAAAGGAAAC CATCGAAATC GGTCCGCTGA ATGTTGTCAT 361 TCTCGAAGGC CTCATGCCGT GGTTTGACGA GCGTATCGCG AAACTGTGCA AGCTGAAAAT 421 CTTCGTCGAA GCGACTGGCG AGGAGCGTCT GATCAGACGC ATTGAGCGCG ATTGGGAACG 481 TGGCCGCGAC GTGGCATCGA TTATCAAGCA ATGGCGTGAA GCAGTCAGCC CAATGTATGA 541 AATTTTCGTT GAAAAGATGA AGCAGAAAGC TGATCTGATC ATTCCGTGGA GCGAGCGTCA 601 CGAGGTTAGC ACCAACGTGC TGGACTTCGC CATCGAGCAC CTGTTCCGTA AACATGTGGA 661 CCCGAACTGA AGAGCTTAGA GACCGGCTCG ATACAGTTTC ACGTCGCTGA GGTGTCAATC 721 GTCGGAGCCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG 781 GGTTTTTTGC ATGGTCATAG CTGTTTCCTG AGAGCTTGGC AGGTGATGAC ACACATTAAC 841 AAATTTCGTG AGGAGTCTCC AGAAGAATGC CATTAATTTC CATAGGCTCC GCCCCCCTGA 901 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 961 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 1021 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 1081 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 1141 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 1201 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 1261 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 1321 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 1381 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 1441 TACGCGCAGA AAAAAAGGAT CTCAAGAAGG CCTACTATTA GCAACAACGA TCCTTTGATC 1501 TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG 1561 AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA 1621 ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA 1681 CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG 1741 ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAA 1801 CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC 1861 AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT 1921 AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC TACAGGCATC 1981 GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT CCGGTTCCCA ACGATCAAGG 2041 CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG TCCTCCGATC 2101 GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG TTATGGCAGC ACTGCATAAT 2161 TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA CTGGTGAGTA CTCAACCAAG 2221 TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT GCCCGGCGTC AATACGGGAT 2281 AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA TTGGAAAACG TTCTTCGGGG 2341 CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT CGATGTAACC CACTCGTGCA 2401 CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA 2461 AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA AATGTTGAAT ACTCATACTC 2521 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 2581 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACCTGCAC CAGTCAGTAA 2641 AACGACGGCC AGTAGTCAAA AGCCTCCGAC CGGAGGCTTT TGACTTGGTC AGGTGGAGTG 2701 GGAG //