LOCUS       Exported     2809 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..2809
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      847..894
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     2742..2758
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      2759..2790
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..775
                     /label="outB"
                     /note="Catalyzes the ATP-dependent amidation of deamido-NAD to form NAD. Uses ammonia as a nitrogen source."
                     /translation="MTKNLLNYLNELEAWLTNYVNEAHAQGVVVGLSGGVDSAVVAAMTKKLFPQNHLTLVMHINNSELDHKATTALVEQLQLNNKQVDLEPPYRAMLQALTIDPQKELMVAGNLKARLRMACLYTHAQKHNYLVLGTGNFIEYSLGYFTKWGDGACDVAPLAFLLKSDVYALSQHFNVPELVIERAPTASLFAGQTDEAEMGLTYKELDQYFQGHLQLSATKQQRVDHLRQSSQHKRSLPKTFKPLYSFQI"
     primer_bind     complement(899..915)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(982..1570)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(1761..2621)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(2622..2726)
                     /note="ampicillin resistance gene promoter"
                     /label="bla promoter"
ORIGIN
        1 AATCAGTTTC TTTGGCAGCC GGTCTCAAAT GACGAAAAAT CTGCTGAATT ACCTGAATGA
       61 GCTGGAGGCT TGGCTGACTA ATTATGTCAA TGAAGCGCAT GCCCAAGGTG TGGTTGTGGG
      121 CCTGTCCGGT GGCGTGGATA GCGCGGTCGT TGCCGCCATG ACCAAGAAGC TGTTCCCGCA
      181 AAATCACTTG ACCCTGGTTA TGCATATTAA TAACAGCGAA TTGGACCATA AAGCAACGAC
      241 GGCGTTAGTG GAACAGCTGC AATTGAATAA CAAGCAAGTC GACCTGGAAC CGCCGTACCG
      301 TGCAATGTTG CAAGCGTTGA CGATCGATCC GCAAAAGGAG CTGATGGTTG CTGGTAATTT
      361 GAAGGCGCGT TTGCGCATGG CGTGTCTGTA TACGCACGCG CAGAAACACA ACTACCTGGT
      421 GCTGGGTACC GGCAATTTCA TCGAATATTC TCTGGGCTAT TTTACGAAGT GGGGTGACGG
      481 TGCTTGTGAT GTCGCCCCGT TGGCGTTCCT GCTGAAAAGC GACGTGTATG CTCTGAGCCA
      541 ACATTTTAAT GTTCCGGAGC TGGTCATCGA GCGTGCACCG ACGGCGAGCC TGTTCGCAGG
      601 CCAAACCGAT GAAGCGGAAA TGGGTCTGAC GTACAAGGAG CTGGACCAGT ACTTTCAGGG
      661 TCACTTGCAG CTGTCAGCGA CGAAACAGCA ACGTGTGGAT CATCTGCGTC AATCTAGCCA
      721 ACATAAACGT AGCCTGCCAA AGACCTTCAA GCCGCTGTAC AGCTTCCAAA TTTGAAGAGC
      781 TTAGAGACCG ACCCGTCCTT TGTTGTGATC GCTGAGGTGT CAATCGTCGG AGCCGCTGAG
      841 CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCATGGT
      901 CATAGCTGTT TCCTGAGAGC TTGGCAGGTG ATGACACACA TTAACAAATT TCGTGAGGAG
      961 TCTCCAGAAG AATGCCATTA ATTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA
     1021 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC
     1081 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC
     1141 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG
     1201 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA
     1261 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC
     1321 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC
     1381 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG
     1441 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA
     1501 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA
     1561 AGGATCTCAA GAAGGCCTAC TATTAGCAAC AACGATCCTT TGATCTTTTC TACGGGGTCT
     1621 GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG
     1681 ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT
     1741 GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC
     1801 TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG
     1861 GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGAACCACG CTCACCGGCT
     1921 CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA
     1981 ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG
     2041 CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG
     2101 TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC
     2161 CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG
     2221 TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG
     2281 CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG
     2341 TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT
     2401 AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG
     2461 ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA
     2521 GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA
     2581 AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT
     2641 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG
     2701 AAAAATAAAC AAATAGGGGT TCCGCGCACC TGCACCAGTC AGTAAAACGA CGGCCAGTAG
     2761 TCAAAAGCCT CCGACCGGAG GCTTTTGACT TGGTCAGGTG GAGTGGGAG
//