LOCUS       Exported     3400 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3400
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1438..1485
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3333..3349
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3350..3381
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..1366
                     /label="MPN572"
                     /note="Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides (By similarity)."
                     /translation="MKLNKLFDSKTVVFKKSDKYGKSDCCKTKCVEGQIEFGVKIPTDFPAFNRALVTFLKTQKNQLNVDLDSFVELYKAENLCYKTALKVVVASITFCETTPFTMKTEPQKNVEVAVKCDSEHTSLIKEYEVVGNYVNMARQLQDTPSDQLYPEEFVKRFEKAATGLGVKITVLKQADLIKKKMGLLLGVNKGSEREARLLVISYNNNKKSSETLALVGKGITYDSGGMNIKTGDYMRGMKYDMSGAAIVCSTVLALAKNKVKTNVVAVAALTENLPGPHAQRPDDIQTAYNGKTVEIDNTDAEGRLVLADAISYAAKDLKATQIIDVATLTGLMSYILSTTYTGIFSTCDMAWDAFKKAACCAGEPVWRLPMHPDYLKPLESKLADLQNSTSVKGAGSSRAACFLAEFREGVPLIHCDIASTASIQDLGQGVLVRTLYERAAQQAKE"
     primer_bind     complement(1490..1506)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1573..2161)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2352..3212)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3213..3317)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 ACAACGAGCA GACCGAATAG GGTCTCAAAT GAAACTGAAC AAATTATTCG ATAGCAAGAC
       61 GGTCGTGTTC AAGAAGTCTG ACAAATACGG TAAGAGCGAT TGCTGCAAGA CCAAATGCGT
      121 TGAGGGCCAA ATTGAGTTCG GTGTCAAGAT CCCGACCGAC TTCCCGGCCT TTAATCGCGC
      181 GCTGGTCACC TTCCTTAAGA CCCAAAAGAA CCAGCTGAAC GTTGACTTGG ACAGCTTCGT
      241 CGAACTGTAT AAAGCTGAGA ACCTGTGCTA TAAGACGGCG CTGAAAGTTG TGGTGGCTAG
      301 CATCACGTTT TGTGAAACCA CGCCGTTTAC GATGAAAACG GAGCCGCAGA AGAATGTCGA
      361 GGTCGCGGTT AAATGTGACT CCGAGCATAC TAGCCTGATC AAGGAGTATG AGGTAGTGGG
      421 CAACTACGTT AACATGGCGC GTCAGCTGCA GGACACGCCG AGCGATCAGC TGTACCCGGA
      481 GGAATTTGTC AAGAGATTCG AAAAAGCCGC AACGGGCCTG GGCGTCAAAA TTACCGTTCT
      541 GAAACAAGCA GATTTAATTA AGAAGAAAAT GGGCCTGCTG CTGGGCGTGA ATAAAGGTAG
      601 CGAACGCGAG GCGAGACTGC TGGTGATTAG CTACAACAAT AACAAAAAGA GCTCGGAAAC
      661 GCTGGCGTTG GTCGGTAAGG GCATTACGTA CGACAGCGGT GGCATGAACA TTAAAACGGG
      721 TGATTACATG CGTGGTATGA AGTATGATAT GAGCGGCGCC GCGATTGTGT GCTCCACGGT
      781 ACTGGCGTTG GCGAAGAATA AAGTGAAAAC CAATGTTGTG GCAGTGGCGG CGTTGACCGA
      841 AAATCTGCCG GGCCCGCATG CGCAGCGTCC GGATGACATC CAAACCGCAT ACAACGGTAA
      901 AACGGTTGAA ATTGATAATA CCGACGCAGA AGGTCGTCTG GTTCTGGCGG ATGCAATCTC
      961 TTATGCGGCC AAAGATCTGA AAGCGACCCA AATTATCGAT GTGGCTACCC TCACCGGCTT
     1021 AATGAGCTAC ATTTTATCAA CCACCTACAC CGGTATCTTC AGCACCTGTG ATATGGCATG
     1081 GGATGCATTC AAAAAGGCCG CATGCTGCGC GGGTGAACCA GTGTGGAGAC TTCCGATGCA
     1141 CCCTGATTAT CTGAAACCGC TGGAGTCCAA GTTGGCGGAT CTGCAAAACA GCACGTCCGT
     1201 GAAGGGTGCG GGTTCGAGCC GTGCGGCGTG TTTCCTGGCC GAGTTTCGTG AAGGTGTGCC
     1261 ACTGATTCAC TGCGATATTG CGAGCACCGC GAGCATTCAA GACTTGGGCC AAGGTGTGCT
     1321 GGTCCGTACC CTGTACGAAC GTGCGGCTCA GCAGGCCAAG GAGTGAAGAG CTTAGAGACC
     1381 CGGATTCTAG GTGCAACTCT CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA
     1441 GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT
     1501 TTCCTGAGAG CTTGGCAGGT GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA
     1561 GAATGCCATT AATTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC
     1621 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG
     1681 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT
     1741 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA
     1801 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC
     1861 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC
     1921 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT
     1981 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT
     2041 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC
     2101 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA
     2161 AGAAGGCCTA CTATTAGCAA CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG
     2221 TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC
     2281 TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT
     2341 TGGTCTGACA GTTACCAATG CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT
     2401 CGTTCATCCA TAGTTGCCTG ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA
     2461 CCATCTGGCC CCAGTGCTGC AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA
     2521 TCAGCAATAA ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC
     2581 GCCTCCATCC AGTCTATTAA TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT
     2641 AGTTTGCGCA ACGTTGTTGC CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT
     2701 ATGGCTTCAT TCAGCTCCGG TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG
     2761 TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA
     2821 GTGTTATCAC TCATGGTTAT GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA
     2881 AGATGCTTTT CTGTGACTGG TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG
     2941 CGACCGAGTT GCTCTTGCCC GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT
     3001 TTAAAAGTGC TCATCATTGG AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG
     3061 CTGTTGAGAT CCAGTTCGAT GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT
     3121 ACTTTCACCA GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA
     3181 ATAAGGGCGA CACGGAAATG TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC
     3241 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA
     3301 CAAATAGGGG TTCCGCGCAC CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC
     3361 TCCGACCGGA GGCTTTTGAC TTGGTCAGGT GGAGTGGGAG
//