LOCUS       Exported     3106 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3106
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1144..1191
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3039..3055
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3056..3087
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..1072
                     /label="MPN586"
                     /note=""
                     /translation="MRQTIYLTVNLMQNWVERWILDWANILTWKTLCHQTKIGLPNGAQAFVSAQTSTIPKTAFTAHDFVDYTLPQEQKGKEQKEQQWMKNSQTKDTDVHPYADFAVLEILLFPDSSTDRKIFNHFIQPAIRAYKQLGDSLNIFANQTLDQPKHNRYYLLGYPFLRNKAASLFLNQTEQRKEHMDKTTQILHEKPFVVLTDPAKPTLIRNKGANFTGNTWTSNYDLSKKSGLYHKFLGKKYQIYGKSIVISDLNLSSGSSGSLLLNDRKQIVGIYFGVDGPKDELGFSQLLRWQAKNNDEEKDSVAYDLIFGNKNTTKYYAQFAKEHKTHLYEQIDRSNDQQFTFVKNQKC"
     primer_bind     complement(1196..1212)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1279..1867)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2058..2918)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(2919..3023)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 AATGCGTCAT TTTACACGGC GGTCTCAAAT GCGTCAAACG ATCTACTTGA CCGTCAATTT
       61 GATGCAAAAC TGGGTTGAGC GTTGGATTCT GGACTGGGCT AATATTCTGA CGTGGAAAAC
      121 CCTGTGCCAC CAAACCAAAA TCGGTCTGCC GAATGGCGCG CAAGCGTTTG TCTCCGCGCA
      181 AACGTCTACG ATCCCAAAGA CCGCGTTCAC CGCGCATGAC TTCGTCGACT ATACTCTGCC
      241 GCAGGAACAG AAGGGCAAAG AACAGAAGGA GCAGCAGTGG ATGAAGAATA GCCAAACCAA
      301 GGACACGGAC GTCCATCCGT ATGCGGACTT TGCGGTTCTG GAGATTCTGC TGTTCCCAGA
      361 CAGCAGCACC GATCGCAAAA TTTTCAACCA CTTCATTCAG CCTGCGATCC GTGCGTACAA
      421 ACAGTTAGGC GACAGCCTGA ACATTTTCGC GAATCAGACC CTGGATCAAC CTAAACATAA
      481 CCGTTATTAT TTGCTGGGTT ATCCGTTCCT GAGAAATAAA GCTGCGTCTT TGTTCCTGAA
      541 TCAGACCGAA CAGCGTAAGG AGCATATGGA CAAAACCACG CAGATTCTGC ACGAAAAACC
      601 GTTCGTGGTG CTGACGGATC CGGCGAAACC TACTCTGATT CGTAATAAGG GTGCGAATTT
      661 TACTGGCAAC ACTTGGACTA GCAATTACGA TTTAAGCAAA AAGAGCGGTC TGTATCATAA
      721 ATTCCTGGGC AAGAAATATC AGATCTATGG TAAGTCTATT GTGATCAGCG ACCTGAACTT
      781 GAGCAGCGGT AGCAGCGGTA GCCTGCTGCT GAACGACCGC AAACAGATTG TTGGCATTTA
      841 TTTCGGCGTG GACGGTCCGA AGGATGAGCT GGGTTTTAGC CAACTGCTGC GCTGGCAGGC
      901 CAAGAACAAT GACGAAGAGA AGGACAGCGT TGCATACGAC TTGATTTTCG GTAACAAGAA
      961 CACGACCAAA TACTATGCGC AGTTCGCGAA AGAGCATAAA ACCCATCTGT ACGAACAGAT
     1021 TGATCGTTCG AACGATCAAC AGTTTACGTT CGTCAAGAAC CAGAAGTGCT GAAGAGCTTA
     1081 GAGACCGTGT CGCTCGCAAT CCTAATCGCT GAGGTGTCAA TCGTCGGAGC CGCTGAGCAA
     1141 TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCATGGTCAT
     1201 AGCTGTTTCC TGAGAGCTTG GCAGGTGATG ACACACATTA ACAAATTTCG TGAGGAGTCT
     1261 CCAGAAGAAT GCCATTAATT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG
     1321 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC
     1381 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC
     1441 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC
     1501 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG
     1561 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC
     1621 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA
     1681 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC
     1741 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC
     1801 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG
     1861 ATCTCAAGAA GGCCTACTAT TAGCAACAAC GATCCTTTGA TCTTTTCTAC GGGGTCTGAC
     1921 GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC
     1981 TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG
     2041 TAAACTTGGT CTGACAGTTA CCAATGCTTA ATCAGTGAGG CACCTATCTC AGCGATCTGT
     2101 CTATTTCGTT CATCCATAGT TGCCTGACTC CCCGTCGTGT AGATAACTAC GATACGGGAG
     2161 GGCTTACCAT CTGGCCCCAG TGCTGCAATG ATACCGCGAG AACCACGCTC ACCGGCTCCA
     2221 GATTTATCAG CAATAAACCA GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG TCCTGCAACT
     2281 TTATCCGCCT CCATCCAGTC TATTAATTGT TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA
     2341 GTTAATAGTT TGCGCAACGT TGTTGCCATT GCTACAGGCA TCGTGGTGTC ACGCTCGTCG
     2401 TTTGGTATGG CTTCATTCAG CTCCGGTTCC CAACGATCAA GGCGAGTTAC ATGATCCCCC
     2461 ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG
     2521 GCCGCAGTGT TATCACTCAT GGTTATGGCA GCACTGCATA ATTCTCTTAC TGTCATGCCA
     2581 TCCGTAAGAT GCTTTTCTGT GACTGGTGAG TACTCAACCA AGTCATTCTG AGAATAGTGT
     2641 ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG TCAATACGGG ATAATACCGC GCCACATAGC
     2701 AGAACTTTAA AAGTGCTCAT CATTGGAAAA CGTTCTTCGG GGCGAAAACT CTCAAGGATC
     2761 TTACCGCTGT TGAGATCCAG TTCGATGTAA CCCACTCGTG CACCCAACTG ATCTTCAGCA
     2821 TCTTTTACTT TCACCAGCGT TTCTGGGTGA GCAAAAACAG GAAGGCAAAA TGCCGCAAAA
     2881 AAGGGAATAA GGGCGACACG GAAATGTTGA ATACTCATAC TCTTCCTTTT TCAATATTAT
     2941 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA
     3001 AATAAACAAA TAGGGGTTCC GCGCACCTGC ACCAGTCAGT AAAACGACGG CCAGTAGTCA
     3061 AAAGCCTCCG ACCGGAGGCT TTTGACTTGG TCAGGTGGAG TGGGAG
//