LOCUS       Exported     3124 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3124
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1162..1209
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3057..3073
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3074..3105
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..1090
                     /label="MPN591"
                     /note=""
                     /translation="MANSLRNVNDYDPFKYRPSYFDLEAPTESFALGKFVDAIEVKQMALDAFSESSMVSIATSQLPKTAYTATSFIESEYYSFPYLFNDQKYYWDYFDYKIPAADFAVLELELDLNNQQDQQIKDHFIDPAIKAYKQLGDSTGLFATKPLTEYQNDTHYLLGYPVVPTDHTQLWECKQGAERFSYGYFYSNMARLTKNLRQGDPNAGSKTHIEYSNELLDKDSMDQGIVRFSTFLGANINYHDYDYRQQGYGLTLTDTNLPGGSSGSLVFNQDKKISSIYSAATESDSVGYAQLLRTPRDVNGISVVSQSYDLIFGDSNTKRYYAMFAKKQQTHLYSEILKSTDEQYRYVVDKQFN"
     primer_bind     complement(1214..1230)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1297..1885)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2076..2936)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(2937..3041)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 ACAAGGAGTC GGCATATCAC GGTCTCAAAT GGCTAACTCC CTGCGTAACG TCAATGATTA
       61 CGATCCGTTC AAGTACCGTC CGTCTTATTT TGACTTGGAG GCCCCGACGG AGAGCTTCGC
      121 GCTGGGCAAG TTTGTTGATG CAATCGAGGT TAAACAAATG GCATTGGATG CATTCAGCGA
      181 ATCCTCCATG GTTTCGATCG CTACGAGCCA ACTGCCTAAG ACGGCGTATA CCGCCACGAG
      241 CTTCATCGAG TCTGAGTACT ATAGCTTCCC TTATTTGTTT AACGACCAGA AGTACTACTG
      301 GGATTATTTC GACTATAAGA TTCCGGCGGC TGATTTTGCC GTTCTGGAGC TGGAGTTGGA
      361 TCTGAATAAC CAACAGGATC AGCAGATCAA GGATCATTTC ATTGATCCTG CCATTAAAGC
      421 GTACAAACAA CTGGGTGATA GCACCGGTCT GTTCGCAACC AAACCGCTGA CGGAGTATCA
      481 GAACGACACT CACTACCTCC TCGGCTATCC GGTAGTCCCA ACGGACCACA CTCAGTTGTG
      541 GGAATGTAAG CAAGGTGCGG AACGCTTCTC GTACGGCTAC TTCTACTCTA ATATGGCTCG
      601 TCTTACCAAA AATCTGCGTC AGGGTGATCC GAATGCCGGC TCTAAAACCC ATATTGAGTA
      661 CAGCAATGAA TTATTAGACA AAGATAGCAT GGATCAGGGT ATTGTCCGTT TTTCTACCTT
      721 CCTGGGTGCA AACATTAACT ACCACGACTA CGATTACCGC CAGCAGGGTT ACGGCCTGAC
      781 CCTGACCGAC ACCAATCTGC CTGGTGGCAG CAGCGGTAGC CTGGTTTTTA ATCAGGATAA
      841 GAAGATCAGC AGCATTTACA GCGCAGCAAC CGAGAGCGAT TCTGTTGGCT ACGCCCAGTT
      901 GCTGCGCACG CCGCGTGACG TTAATGGCAT CAGCGTTGTT AGCCAGAGCT ACGACCTGAT
      961 CTTTGGCGAC TCAAACACCA AGCGCTATTA CGCCATGTTT GCGAAAAAAC AGCAAACGCA
     1021 CTTGTACTCC GAAATCCTGA AAAGCACCGA TGAACAGTAC CGCTATGTCG TTGATAAGCA
     1081 GTTCAACTGA AGAGCTTAGA GACCCAGTCC TTGCCAATCG AAGTCGCTGA GGTGTCAATC
     1141 GTCGGAGCCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG
     1201 GGTTTTTTGC ATGGTCATAG CTGTTTCCTG AGAGCTTGGC AGGTGATGAC ACACATTAAC
     1261 AAATTTCGTG AGGAGTCTCC AGAAGAATGC CATTAATTTC CATAGGCTCC GCCCCCCTGA
     1321 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG
     1381 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT
     1441 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG
     1501 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC
     1561 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT
     1621 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA
     1681 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC
     1741 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC
     1801 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT
     1861 TACGCGCAGA AAAAAAGGAT CTCAAGAAGG CCTACTATTA GCAACAACGA TCCTTTGATC
     1921 TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG
     1981 AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA
     2041 ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA
     2101 CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG
     2161 ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAA
     2221 CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC
     2281 AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT
     2341 AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC TACAGGCATC
     2401 GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT CCGGTTCCCA ACGATCAAGG
     2461 CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG TCCTCCGATC
     2521 GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG TTATGGCAGC ACTGCATAAT
     2581 TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA CTGGTGAGTA CTCAACCAAG
     2641 TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT GCCCGGCGTC AATACGGGAT
     2701 AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA TTGGAAAACG TTCTTCGGGG
     2761 CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT CGATGTAACC CACTCGTGCA
     2821 CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA
     2881 AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA AATGTTGAAT ACTCATACTC
     2941 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA
     3001 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACCTGCAC CAGTCAGTAA
     3061 AACGACGGCC AGTAGTCAAA AGCCTCCGAC CGGAGGCTTT TGACTTGGTC AGGTGGAGTG
     3121 GGAG
//