LOCUS Exported 2431 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..2431 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 469..516 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 2364..2380 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 2381..2412 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 29..397 /label="MPN593" /note="" /translation="MNKKESTTTKKQWFKKCSFKKLKAEICNMLPTTPHNTKRTLIWVIVFSFITFLSFIFAYVCFNYAPVSTGFLYFLGAVFLLIGFAFAILSFVAMVKFVADYFANRFSNTQLKMDCDCAKTKK" primer_bind complement(521..537) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(604..1192) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(1383..2243) /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" promoter complement(2244..2348) /label="bla promoter" /note="ampicillin resistance gene promoter" ORIGIN 1 AAGGAACTAT GGCATCGAGC GGTCTCAAAT GAACAAGAAG GAATCTACCA CGACCAAGAA 61 ACAGTGGTTT AAGAAATGTT CCTTCAAGAA ATTGAAAGCG GAGATCTGTA ACATGCTGCC 121 GACCACCCCT CACAACACGA AGCGCACCCT GATTTGGGTT ATCGTTTTTT CTTTTATTAC 181 CTTCCTGAGC TTCATCTTCG CGTACGTGTG TTTTAATTAT GCGCCGGTTA GCACCGGCTT 241 TCTGTATTTC CTGGGCGCCG TGTTTCTGTT AATCGGTTTC GCGTTTGCTA TCCTGAGCTT 301 TGTGGCTATG GTAAAATTTG TGGCCGACTA CTTCGCGAAC CGTTTTAGCA ACACTCAACT 361 GAAGATGGAT TGCGACTGCG CAAAAACTAA GAAGTGAAGA GCTTAGAGAC CGGTTCCTAG 421 ACCTCCTGGA TCGCTGAGGT GTCAATCGTC GGAGCCGCTG AGCAATAACT AGCATAACCC 481 CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCATG GTCATAGCTG TTTCCTGAGA 541 GCTTGGCAGG TGATGACACA CATTAACAAA TTTCGTGAGG AGTCTCCAGA AGAATGCCAT 601 TAATTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 661 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 721 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 781 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 841 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 901 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 961 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 1021 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 1081 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 1141 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGGCCT 1201 ACTATTAGCA ACAACGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA 1261 AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT 1321 TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC 1381 AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC 1441 ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT ACCATCTGGC 1501 CCCAGTGCTG CAATGATACC GCGAGAACCA CGCTCACCGG CTCCAGATTT ATCAGCAATA 1561 AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC 1621 CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC 1681 AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT CGTCGTTTGG TATGGCTTCA 1741 TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGCAAAAAA 1801 GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA 1861 CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT 1921 TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT 1981 TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG 2041 CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA 2101 TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC 2161 AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG 2221 ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 2281 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 2341 GTTCCGCGCA CCTGCACCAG TCAGTAAAAC GACGGCCAGT AGTCAAAAGC CTCCGACCGG 2401 AGGCTTTTGA CTTGGTCAGG TGGAGTGGGA G //