LOCUS Exported 3490 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..3490 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 1528..1575 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 3423..3439 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 3440..3471 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 29..1456 /note="Produces ATP from ADP in the presence of a proton gradient across the membrane. The catalytic sites are hosted primarily by the beta subunits." /translation="MKKENITYGKVHQVIGPVVDVIFTESSQLPRIYDCLSVKLAGEELFLEAAQLIGDDIVRCIALGPTEGLARNEKVTNYNHPIEVPVGKNVLGRMFNVLGKPIDGKEELPKKPQLPIHRKPPSFDDQSNTLEIFETGIKVIDLLTPYARGGKIGLFGGAGVGKTVLVQELIHNIAKEHSGLSVFAGVGERTREGNDLYYEMIQGGVIDKTALVFGQMNEPPGARMRVALTALTMAEYFRDHDNQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQPTLATEMGQLQERIASTKTGSITSVQAIYVPADDLTDPAPATTFTHLDAKTVLDRNIAALGIFPAINPLESTSRLLDPNIVGINHYKVALGVQNILQRFAELQDIIAILGIDELADEDKIIVERARRIRNFLSQPFFVAEKFSGIAGKYVPLSDTIQSFKEILDGKHDDLPEQAFFFVGTIQEAVEKAKRLKKATVEEK" /label="atpD" primer_bind complement(1580..1596) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(1663..2251) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(2442..3302) /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" promoter complement(3303..3407) /label="bla promoter" /note="ampicillin resistance gene promoter" ORIGIN 1 AATGCGTCAT TTTACACGGC GGTCTCAAAT GAAGAAAGAA AACATCACTT ATGGTAAAGT 61 CCATCAGGTC ATCGGCCCGG TTGTGGACGT TATCTTCACG GAGAGCTCCC AGCTGCCGCG 121 TATCTACGAC TGTCTGAGCG TGAAACTGGC CGGTGAGGAG CTGTTCCTGG AGGCTGCACA 181 ACTGATCGGT GACGATATCG TGCGTTGTAT CGCCCTGGGC CCGACCGAGG GTTTGGCACG 241 CAATGAGAAA GTTACCAACT ACAACCACCC GATCGAAGTG CCGGTCGGTA AAAATGTTCT 301 GGGTCGTATG TTCAATGTTC TGGGCAAACC GATTGATGGT AAGGAGGAGC TGCCGAAAAA 361 GCCGCAGCTG CCGATTCATC GTAAGCCGCC GAGCTTCGAT GATCAGAGCA ACACGTTGGA 421 GATTTTCGAA ACCGGTATTA AAGTTATTGA CTTACTCACT CCTTACGCGC GCGGTGGCAA 481 AATCGGTCTG TTCGGCGGCG CAGGCGTCGG TAAAACTGTT TTGGTGCAGG AACTGATCCA 541 CAATATCGCG AAGGAACACA GCGGCCTGAG CGTTTTCGCC GGCGTCGGTG AACGCACGCG 601 TGAAGGTAAC GACCTGTACT ACGAAATGAT TCAAGGCGGC GTGATTGATA AGACCGCGCT 661 GGTCTTTGGT CAAATGAACG AGCCGCCAGG TGCTCGTATG CGTGTGGCCC TGACCGCGCT 721 GACCATGGCG GAGTACTTCC GTGATCATGA TAACCAGGAC GTTCTGCTGT TCATTGACAA 781 CATCTTTCGT TTCACGCAGG CGGGTTCTGA AGTCTCTGCT CTGCTGGGTA GAATGCCGTC 841 CGCAGTTGGC TACCAGCCGA CGCTGGCAAC CGAAATGGGT CAGCTGCAGG AACGTATCGC 901 TAGCACCAAA ACCGGTTCGA TCACCAGCGT ACAGGCGATC TATGTGCCGG CAGATGATCT 961 GACGGACCCG GCTCCGGCCA CCACGTTTAC GCATCTGGAC GCAAAGACGG TGCTGGATCG 1021 CAATATCGCG GCGCTGGGTA TTTTCCCAGC CATTAATCCG CTGGAGTCCA CCTCCCGCTT 1081 GCTGGATCCG AACATTGTTG GTATCAATCA CTACAAAGTT GCGCTGGGTG TTCAGAATAT 1141 CCTGCAGCGT TTCGCTGAGC TGCAAGACAT TATCGCGATT CTGGGTATCG ATGAACTGGC 1201 TGACGAGGAC AAGATCATCG TTGAGAGAGC CCGTCGCATT CGCAACTTCC TGAGCCAGCC 1261 GTTTTTCGTA GCGGAAAAAT TTAGCGGTAT TGCGGGTAAG TACGTCCCGC TGAGCGATAC 1321 CATTCAAAGC TTTAAGGAGA TTCTGGATGG CAAACATGAC GATTTGCCTG AGCAGGCGTT 1381 TTTCTTTGTG GGTACGATTC AGGAGGCGGT TGAAAAAGCT AAACGTCTGA AAAAAGCCAC 1441 TGTCGAGGAG AAGTGAAGAG CTTAGAGACC CTGGAATCAG CCTTGTGTCT CGCTGAGGTG 1501 TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC 1561 TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT GATGACACAC 1621 ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA GGCTCCGCCC 1681 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 1741 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 1801 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 1861 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 1921 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 1981 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 2041 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 2101 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 2161 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 2221 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA CAACGATCCT 2281 TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG 2341 GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT 2401 AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG CTTAATCAGT 2461 GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG ACTCCCCGTC 2521 GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC AATGATACCG 2581 CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC CGGAAGGGCC 2641 GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA TTGTTGCCGG 2701 GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC CATTGCTACA 2761 GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG TTCCCAACGA 2821 TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT 2881 CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT GGCAGCACTG 2941 CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG TGAGTACTCA 3001 ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC GGCGTCAATA 3061 CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG AAAACGTTCT 3121 TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT GTAACCCACT 3181 CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG GTGAGCAAAA 3241 ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG TTGAATACTC 3301 ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 3361 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC CTGCACCAGT 3421 CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC TTGGTCAGGT 3481 GGAGTGGGAG //