LOCUS       Exported     3490 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3490
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1528..1575
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3423..3439
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3440..3471
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..1456
                     /note="Produces ATP from ADP in the presence of a proton gradient across the membrane. The catalytic sites are hosted primarily by the beta subunits."
                     /translation="MKKENITYGKVHQVIGPVVDVIFTESSQLPRIYDCLSVKLAGEELFLEAAQLIGDDIVRCIALGPTEGLARNEKVTNYNHPIEVPVGKNVLGRMFNVLGKPIDGKEELPKKPQLPIHRKPPSFDDQSNTLEIFETGIKVIDLLTPYARGGKIGLFGGAGVGKTVLVQELIHNIAKEHSGLSVFAGVGERTREGNDLYYEMIQGGVIDKTALVFGQMNEPPGARMRVALTALTMAEYFRDHDNQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQPTLATEMGQLQERIASTKTGSITSVQAIYVPADDLTDPAPATTFTHLDAKTVLDRNIAALGIFPAINPLESTSRLLDPNIVGINHYKVALGVQNILQRFAELQDIIAILGIDELADEDKIIVERARRIRNFLSQPFFVAEKFSGIAGKYVPLSDTIQSFKEILDGKHDDLPEQAFFFVGTIQEAVEKAKRLKKATVEEK"
                     /label="atpD"
     primer_bind     complement(1580..1596)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1663..2251)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2442..3302)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3303..3407)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 AATGCGTCAT TTTACACGGC GGTCTCAAAT GAAGAAAGAA AACATCACTT ATGGTAAAGT
       61 CCATCAGGTC ATCGGCCCGG TTGTGGACGT TATCTTCACG GAGAGCTCCC AGCTGCCGCG
      121 TATCTACGAC TGTCTGAGCG TGAAACTGGC CGGTGAGGAG CTGTTCCTGG AGGCTGCACA
      181 ACTGATCGGT GACGATATCG TGCGTTGTAT CGCCCTGGGC CCGACCGAGG GTTTGGCACG
      241 CAATGAGAAA GTTACCAACT ACAACCACCC GATCGAAGTG CCGGTCGGTA AAAATGTTCT
      301 GGGTCGTATG TTCAATGTTC TGGGCAAACC GATTGATGGT AAGGAGGAGC TGCCGAAAAA
      361 GCCGCAGCTG CCGATTCATC GTAAGCCGCC GAGCTTCGAT GATCAGAGCA ACACGTTGGA
      421 GATTTTCGAA ACCGGTATTA AAGTTATTGA CTTACTCACT CCTTACGCGC GCGGTGGCAA
      481 AATCGGTCTG TTCGGCGGCG CAGGCGTCGG TAAAACTGTT TTGGTGCAGG AACTGATCCA
      541 CAATATCGCG AAGGAACACA GCGGCCTGAG CGTTTTCGCC GGCGTCGGTG AACGCACGCG
      601 TGAAGGTAAC GACCTGTACT ACGAAATGAT TCAAGGCGGC GTGATTGATA AGACCGCGCT
      661 GGTCTTTGGT CAAATGAACG AGCCGCCAGG TGCTCGTATG CGTGTGGCCC TGACCGCGCT
      721 GACCATGGCG GAGTACTTCC GTGATCATGA TAACCAGGAC GTTCTGCTGT TCATTGACAA
      781 CATCTTTCGT TTCACGCAGG CGGGTTCTGA AGTCTCTGCT CTGCTGGGTA GAATGCCGTC
      841 CGCAGTTGGC TACCAGCCGA CGCTGGCAAC CGAAATGGGT CAGCTGCAGG AACGTATCGC
      901 TAGCACCAAA ACCGGTTCGA TCACCAGCGT ACAGGCGATC TATGTGCCGG CAGATGATCT
      961 GACGGACCCG GCTCCGGCCA CCACGTTTAC GCATCTGGAC GCAAAGACGG TGCTGGATCG
     1021 CAATATCGCG GCGCTGGGTA TTTTCCCAGC CATTAATCCG CTGGAGTCCA CCTCCCGCTT
     1081 GCTGGATCCG AACATTGTTG GTATCAATCA CTACAAAGTT GCGCTGGGTG TTCAGAATAT
     1141 CCTGCAGCGT TTCGCTGAGC TGCAAGACAT TATCGCGATT CTGGGTATCG ATGAACTGGC
     1201 TGACGAGGAC AAGATCATCG TTGAGAGAGC CCGTCGCATT CGCAACTTCC TGAGCCAGCC
     1261 GTTTTTCGTA GCGGAAAAAT TTAGCGGTAT TGCGGGTAAG TACGTCCCGC TGAGCGATAC
     1321 CATTCAAAGC TTTAAGGAGA TTCTGGATGG CAAACATGAC GATTTGCCTG AGCAGGCGTT
     1381 TTTCTTTGTG GGTACGATTC AGGAGGCGGT TGAAAAAGCT AAACGTCTGA AAAAAGCCAC
     1441 TGTCGAGGAG AAGTGAAGAG CTTAGAGACC CTGGAATCAG CCTTGTGTCT CGCTGAGGTG
     1501 TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC
     1561 TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT GATGACACAC
     1621 ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA GGCTCCGCCC
     1681 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT
     1741 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT
     1801 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG
     1861 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA
     1921 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA
     1981 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC
     2041 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG
     2101 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG
     2161 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA
     2221 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA CAACGATCCT
     2281 TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG
     2341 GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT
     2401 AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG CTTAATCAGT
     2461 GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG ACTCCCCGTC
     2521 GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC AATGATACCG
     2581 CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC CGGAAGGGCC
     2641 GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA TTGTTGCCGG
     2701 GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC CATTGCTACA
     2761 GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG TTCCCAACGA
     2821 TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT
     2881 CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT GGCAGCACTG
     2941 CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG TGAGTACTCA
     3001 ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC GGCGTCAATA
     3061 CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG AAAACGTTCT
     3121 TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT GTAACCCACT
     3181 CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG GTGAGCAAAA
     3241 ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG TTGAATACTC
     3301 ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA
     3361 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC CTGCACCAGT
     3421 CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC TTGGTCAGGT
     3481 GGAGTGGGAG
//