LOCUS       Exported     3433 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3433
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1471..1518
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3366..3382
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 for"
     terminator      3383..3414
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..1399
                     /translation="MSAQTGTDLFKIADLFAYQVFDSRGFPTVACVVKLASGHTGEAMVPSGASTGEKEAIELRDGDPKAYFGKGVSQAVQNVNQTIAPKLIGLNATDQAAIDALMIQLDGTPNKAKLGANAILAVSLAVAKAAASAQKTSLFKYLANQVMGLNKTEFILTVPMLNVINGGAHADNNIDFQEFMIMPLGANSMHQALKMASETFHALQKLLKQRGLNTNKGDEGGFAPNLKLAEEALDLMVEAIKAAGYQPGSDIAIALDVAASEFYDDTTKRYVFKKGIKAKILDEKEWSLTTAQMIAYLKKLTEQYPIISIEDGLSEHDWEGMETLTKTLGQHIQIVGDDLYCTNPAIAEKGVAHKATNSILIKLNQIGTLTETIKAINIAKDANWSQVISHRSGETEDTTIADLAVAACTGQIKTGSMSRSERIAKYNRLLQIELELGNNAKYLGWNTFKNIKPQKA"
                     /label="eno"
                     /note="Catalyzes the reversible conversion of 2-phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis."
     primer_bind     complement(1523..1539)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1606..2194)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2385..3245)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3246..3350)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 AAAGGGGCCG TCAATATCAG GGTCTCAAAT GTCTGCGCAG ACCGGTACCG ATCTGTTCAA
       61 AATTGCGGAC CTGTTTGCGT ACCAGGTGTT TGACTCGCGT GGCTTTCCGA CCGTGGCATG
      121 TGTGGTGAAA CTGGCAAGCG GTCATACCGG CGAGGCAATG GTACCGTCTG GTGCGAGCAC
      181 CGGTGAGAAA GAGGCAATCG AGCTGCGTGA CGGTGACCCG AAGGCGTATT TCGGTAAGGG
      241 CGTCAGCCAA GCAGTTCAGA ATGTTAACCA GACCATTGCG CCGAAACTGA TCGGTCTGAA
      301 CGCAACCGAC CAGGCGGCGA TCGACGCGCT GATGATCCAA TTGGATGGTA CCCCTAATAA
      361 GGCTAAGCTT GGTGCTAACG CAATCCTGGC CGTCAGCCTG GCGGTAGCAA AAGCGGCCGC
      421 ATCAGCGCAA AAAACGAGCC TGTTTAAATA TCTGGCGAAC CAAGTCATGG GTCTGAATAA
      481 AACTGAGTTC ATCCTGACGG TGCCGATGCT GAACGTCATT AACGGTGGTG CACACGCGGA
      541 TAATAATATC GATTTCCAAG AATTTATGAT CATGCCGCTG GGTGCGAATA GCATGCACCA
      601 AGCTCTGAAG ATGGCTTCCG AAACCTTTCA CGCGCTGCAG AAGCTGCTGA AGCAACGCGG
      661 TCTGAATACC AACAAGGGTG ATGAGGGCGG TTTTGCCCCG AACCTGAAAC TGGCAGAAGA
      721 GGCCTTGGAC CTGATGGTCG AAGCGATTAA AGCAGCCGGT TATCAACCGG GTAGCGATAT
      781 CGCGATCGCG TTGGACGTCG CCGCGTCAGA GTTCTATGAT GACACGACCA AGCGTTACGT
      841 GTTCAAAAAG GGCATCAAGG CCAAGATCCT GGATGAGAAA GAATGGAGCC TGACGACCGC
      901 GCAGATGATT GCATATCTGA AAAAGCTGAC CGAACAATAC CCGATCATCA GCATCGAGGA
      961 CGGTCTGAGC GAGCACGATT GGGAAGGTAT GGAAACCCTG ACCAAGACCC TGGGTCAGCA
     1021 TATTCAGATC GTGGGTGATG ATCTGTACTG TACTAATCCG GCTATCGCAG AGAAGGGTGT
     1081 GGCACATAAA GCTACCAATA GCATCCTGAT TAAACTGAAC CAAATCGGCA CGCTGACCGA
     1141 AACGATTAAA GCCATTAATA TCGCGAAGGA TGCAAATTGG AGCCAAGTGA TCTCCCACCG
     1201 TAGCGGTGAA ACCGAGGACA CCACGATCGC GGATCTGGCC GTGGCGGCGT GTACCGGTCA
     1261 GATTAAAACC GGTTCTATGA GCCGCAGCGA ACGCATTGCC AAATACAACC GCCTGCTGCA
     1321 GATCGAACTG GAACTGGGTA ACAATGCCAA GTACCTGGGT TGGAACACCT TTAAAAACAT
     1381 TAAACCGCAG AAGGCCTGAA GAGCTTAGAG ACCCCATCTT GCGTCGTGAG AATCGCTGAG
     1441 GTGTCAATCG TCGGAGCCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG
     1501 GTCTTGAGGG GTTTTTTGCA TGGTCATAGC TGTTTCCTGA GAGCTTGGCA GGTGATGACA
     1561 CACATTAACA AATTTCGTGA GGAGTCTCCA GAAGAATGCC ATTAATTTCC ATAGGCTCCG
     1621 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG
     1681 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC
     1741 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA
     1801 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT
     1861 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC
     1921 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG
     1981 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC
     2041 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT
     2101 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA
     2161 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGGC CTACTATTAG CAACAACGAT
     2221 CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT
     2281 TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT
     2341 TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC
     2401 AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC
     2461 GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA
     2521 CCGCGAGAAC CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG
     2581 GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC
     2641 CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT
     2701 ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA
     2761 CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT
     2821 CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA
     2881 CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC
     2941 TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA
     3001 ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT
     3061 TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC
     3121 ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA
     3181 AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA
     3241 CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC
     3301 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACCTGCACC
     3361 AGTCAGTAAA ACGACGGCCA GTAGTCAAAA GCCTCCGACC GGAGGCTTTT GACTTGGTCA
     3421 GGTGGAGTGG GAG
//